Abstract
Both one- and two-dimensional peptide mapping techniques have been used to compare the 180-kilodalton subunits from maize chloroplast and type II nuclear DNA-dependent RNA polymerases (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6). Despite their similar molecular mass, these 180-kilodalton subunits are not the same. Also, two-dimensional tryptic maps of the 160-, 43-, and 28-kilodalton polypeptides from maize type II nuclear RNA polymerase and of the 140-, 42-, and 27-kilodalton polypeptides from maize chloroplast RNA polymerase show that each of these six polypeptides is unique.
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Selected References
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- Apel K., Bogorad L. Light-induced increase in the activity of maize plastid DNA-dependent RNA polymerase. Eur J Biochem. 1976 Aug 16;67(2):615–620. doi: 10.1111/j.1432-1033.1976.tb10727.x. [DOI] [PubMed] [Google Scholar]
- Arens M. Q., Stout E. R. Enhanced Activity of the Soluble Ribonucleic Acid Polymerase from 2,4-Dichlorophenoxyacetic Acid-treated Maize Seedlings. Plant Physiol. 1972 Nov;50(5):640–641. doi: 10.1104/pp.50.5.640. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bogorad L. Evolution of organelles and eukaryotic genomes. Science. 1975 May 30;188(4191):891–898. doi: 10.1126/science.1138359. [DOI] [PubMed] [Google Scholar]
- Bottomley W., Smith H. J., Bogorad L. RNA polymerases of maize: partial purification and properties of the chloroplast enzyme. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2412–2416. doi: 10.1073/pnas.68.10.2412. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
- Elder J. H., Pickett R. A., 2nd, Hampton J., Lerner R. A. Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein. J Biol Chem. 1977 Sep 25;252(18):6510–6515. [PubMed] [Google Scholar]
- Grebanier A. E., Coen D. M., Rich A., Bogorad L. Membrane proteins synthesized but not processed by isolated maize chloroplasts. J Cell Biol. 1978 Sep;78(3):734–746. doi: 10.1083/jcb.78.3.734. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jendrisak J., Guilfoyle T. J. Eukaryotic RNA polymerase: comparative subunit structures, immunological properties, and alpha-amanitin sensitivities of the class II enzymes from higher plants. Biochemistry. 1978 Apr 4;17(7):1322–1327. doi: 10.1021/bi00600a029. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Link G., Kidd G. H., Richter G., Bogorad L. Structural relationships among the multiple forms of DNA-dependent RNA polymerase II from cultured parsley cells. Eur J Biochem. 1978 Nov 15;91(2):363–368. doi: 10.1111/j.1432-1033.1978.tb12688.x. [DOI] [PubMed] [Google Scholar]
- Luna E. J., Kidd G. H., Branton D. Identification by peptide analysis of the spectrin-binding protein in human erythrocytes. J Biol Chem. 1979 Apr 10;254(7):2526–2532. [PubMed] [Google Scholar]
- Mullinix K. P., Strain G. C., Bogorad L. RNA polymerases of maize. Purification and molecular structure of DNA-dependent RNA polymerase II. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2386–2390. doi: 10.1073/pnas.70.8.2386. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith H. J., Bogorad L. The polypeptide subunit structure of the DNA-dependent RNA polymerase of Zea mays chloroplasts. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4839–4842. doi: 10.1073/pnas.71.12.4839. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strain G. C., Mullinix K. P., Bogorad L. RNA polymerases of maize: nuclear RNA polymerases. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2647–2651. doi: 10.1073/pnas.68.11.2647. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]