Figure 7.
Effect of –COOH SNPs at different sizes on CD8+ T cell responses under suboptimal induction. (A and B) SNPs with –COOH functional group at different sizes, including SNTXSPTrg4PEGCOOH (33 nm), LTM40PTrg4PEGCOOH (51 nm), SNT20LPTrg4PEGCOOH (82 nm) and SNTZLPTrg4PEGCOOH (137 nm) were sonicated and then added to OT-I splenocytes (8 × 105 cells/well) at various concentrations (10−1 to 10 μg/ml) prior to SIINFEKL (S) stimulation (10−4 μg/ml) for 2 days. Brefeldin A was added during the last 6 h of incubation. Splenocytes were stained for viability, surface CD8 and intracellular IFNγ or IL-2 expression. The percent of IFNγ+ (A) or IL-2+ (B) cells within CD8+ T cells is presented as the mean % ± SE of triplicate cultures. *p < 0.05 as compared to respective no SNP treatment (0 μg/ml) control. Data are representative of at least two separate experiments.