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. 1979 Oct;76(10):5090–5094. doi: 10.1073/pnas.76.10.5090

Interaction site of Escherichia coli cyclic AMP receptor protein on DNA of galactose operon promoters.

T Taniguchi, M O'Neill, B de Crombrugghe
PMCID: PMC413085  PMID: 228278

Abstract

Cyclic AMP (cAMP) and its receptor protein (CRP) have a dual role in the regulation of the two promoters that control the galactose (gal) operon of Escherichia coli. One promoter, P1, requires cAMP-CRP for activity; the other, P2, is inhibited by these factors. We have examined the interactions site of cAMP-CRP on gal DNA by using two types of protection experiments, involving DNase digestion and methylation by dimethyl sulfate. Our results indicate that cAMP-CRP binds to gal DNA in a segment located between 50 and 24 base pairs preceding the P1 start point for transcription. Although the location of the cAMP-CRP interaction site is clearly different in gal and lac DNA, comparison of the DNA sequences suggests a similar recognition sequence. The location of the cAMP . CRP-binding site in gal further suggests that protein-protein interactions between RNA polymerase and cAMP . CRP play an important role in transcription initiation at the gal and possibly other cAMP-dependent promoters.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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