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. 1979 Oct;76(10):5143–5147. doi: 10.1073/pnas.76.10.5143

Synthesis and processing of human chorionic gonadotropin subunits in cultured choriocarcinoma cells.

R W Ruddon, C A Hanson, N J Addison
PMCID: PMC413096  PMID: 291927

Abstract

Pulse and pulse-chase experiments have identified the presence of partially glycosylated precursors of the alpha and beta subunits of human chorionic gonadotropin (hCG) in cultured JAR choriocarcinoma cells. The alpha subunit precursor has an apparent molecular weight (by sodium dodecyl sulfate/polyacrylamide gel electrophoresis) of 18,000 (compared to 22,000 for fully processed alpha subunit); the beta subunit precursor has an apparent molecular weight of 24,000 (fully processed, 34,000). Both of these precursors appear to have an intracellular half-life of at least 1 hr and to contain the mannose core but not the terminal carbohydrate sequences. Fully processed alpha and beta subunits do not accumulate intracellularly, indicating that further processing of the precursors is followed by rapid secretion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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