Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1979 Oct;76(10):5230–5233. doi: 10.1073/pnas.76.10.5230

Assignment of the gene for cytoplasmic superoxide dismutase (Sod-1) to a region of chromosome 16 and of Hprt to a region of the X chromosome in the mouse

Uta Francke 1, R Thomas Taggart 1
PMCID: PMC413114  PMID: 291939

Abstract

In the search for homologous chromosome regions in man and mouse, the locus for cytoplasmic superoxide dismutase (SOD-1; superoxide:superoxide oxidoreductase, EC 1.15.1.1) is of particular interest. In man, the SOD-1 gene occupies the same subregion of chromosome 21 that causes Down syndrome when present in triplicate. Although not obviously implicated in the pathogenesis, SOD-1 is considered to be a biochemical marker for this aneuploid condition. Using a set of 29 mouse—Chinese hamster somatic cell hybrids, we assign Sod-1 to mouse chromosome 16. Isoelectric focusing permits distinction between mouse and Chinese hamster isozymes, and trypsin/Giemsa banding distinguishes mouse from Chinese hamster chromosomes. The mouse fibroblasts used were derived from a male mouse carrying Searle's T(X;16)16H reciprocal translocation in which chromosomes X and 16 have exchanged parts. Analysis of informative hybrids leads to regional assignment of Sod-1 to the distal half of mouse chromosome 16 (16B4 → ter). Because the Chinese hamster cell line (380) used for cell hybridization is deficient in hypoxanthine phosphoribosyltransferase (HPRT; IMP: pyrophosphate phosphoribosyltransferase, EC 2.4.2.8), that part of the mouse X chromosome carrying the complementing Hprt gene can be identified by selection in hypoxanthine/aminopterin/thymidine medium and counterselection in 8-azaguanine. Mouse Hprt is on the XT translocation product containing the proximal region X cen → XD.

Keywords: somatic cell hybrids, comparative mapping, Searle's T(X;16)16H translocation, hypoxanthine phosphoribosyltransferase, isoelectric focusing

Full text

PDF
5230

Images in this article

5232Image
on p.5232
5232Image
on p.5232

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bakay B., Nyhan W. L. The separation of adenine and hypoxanthine-guanine phosphoribosyl transferases isoenzymes by disc gel electrophoresis. Biochem Genet. 1971 Feb;5(1):81–90. doi: 10.1007/BF00485733. [DOI] [PubMed] [Google Scholar]
  2. Cook P. J., Burgerhout W. G. 4th International Workshop on Human Gene Mapping. Report of the committee on the genetic constitution of chromosome 1. Cytogenet Cell Genet. 1978;22(1-6):61–73. doi: 10.1159/000130918. [DOI] [PubMed] [Google Scholar]
  3. Eicher E. M., Nesbitt M. N., Francke U. Cytological identification of the chromosomes involved in Searle's translocation and the location of the centromere in the X chromosome of the mouse. Genetics. 1972 Aug;71(4):643–648. doi: 10.1093/genetics/71.4.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Francke U., Lalley P. A., Moss W., Ivy J., Minna J. D. Gene mapping in Mus musculus by interspecific cell hybridization: assignment of the genes for tripeptidase-1 to chromosome 10, dipeptidase-2 to chromosome 18, acid phosphatase-1 to chromosome 12, and adenylate kinase-1 to chromosome 2. Cytogenet Cell Genet. 1977;19(2-3):57–84. doi: 10.1159/000130799. [DOI] [PubMed] [Google Scholar]
  5. Francke U. The human gene for beta glucuronidase is on chromosome 7. Am J Hum Genet. 1976 Jul;28(4):357–362. [PMC free article] [PubMed] [Google Scholar]
  6. Hagemeijer A., Smit E. M. Partial trisomy 21. Further evidence that trisomy of band 21q22 is essential for Down's phenotype. Hum Genet. 1977 Aug 31;38(1):15–23. doi: 10.1007/BF00295803. [DOI] [PubMed] [Google Scholar]
  7. Kozak C. A., Ruddle F. H. Assignment of the genes for thymidine kinase and galactokinase to Mus musculus chromosome 11 and the preferential segregation of this chromosome in Chinese hamster/mouse somatic cell hybrids. Somatic Cell Genet. 1977 Mar;3(2):121–133. doi: 10.1007/BF01551809. [DOI] [PubMed] [Google Scholar]
  8. LITTLEFIELD J. W. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. doi: 10.1126/science.145.3633.709. [DOI] [PubMed] [Google Scholar]
  9. Lalley P. A., Francke U., Minna J. D. Homologous genes for enolase, phosphogluconate dehydrogenase, phosphoglucomutase, and adenylate kinase are syntenic on mouse chromosome 4 and human chromosome 1p. Proc Natl Acad Sci U S A. 1978 May;75(5):2382–2386. doi: 10.1073/pnas.75.5.2382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lalley P. A., Minna J. D., Francke U. Conservation of autosomal gene synteny groups in mouse and man. Nature. 1978 Jul 13;274(5667):160–163. doi: 10.1038/274160a0. [DOI] [PubMed] [Google Scholar]
  11. Nesbitt M. N., Francke U. A system of nomenclature for band patterns of mouse chromosomes. Chromosoma. 1973;41(2):145–158. doi: 10.1007/BF00319691. [DOI] [PubMed] [Google Scholar]
  12. Report of the committee on comparative mapping. Cytogenet Cell Genet. 1978;22(1-6):150–162. doi: 10.1159/000130927. [DOI] [PubMed] [Google Scholar]
  13. Searle A. G. Clues to homologous regions in mammalian autosomes. Cytogenet Cell Genet. 1976;16(1-5):430–435. doi: 10.1159/000130651. [DOI] [PubMed] [Google Scholar]
  14. Sinet P. M., Couturier J., Dutrillaux B., Poissonnier M., Raoul O., Rethore M. O., Allard D., Lejeune J., Jerome H. Trisomie 21 et superoxyde dismutase-1 (IPO-A). Tentative de localisation sur la sous bande 21Q22.1. Exp Cell Res. 1976 Jan;97:47–55. doi: 10.1016/0014-4827(76)90653-4. [DOI] [PubMed] [Google Scholar]
  15. Weisiger R. A., Fridovich I. Superoxide dismutase. Organelle specificity. J Biol Chem. 1973 May 25;248(10):3582–3592. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES