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. 2013 Jul 31;3(4):158–160. doi: 10.1007/s13659-013-0051-6

Quantitative analysis of trigonelline in some Annona species by proton NMR spectroscopy

Alan Rodrigues Teixeira Machado 1,✉, Gisele Avelar Lage 1, Felipe da Silva Medeiros 1, José Dias de Souza Filho 1, Lúcia Pinheiro Santos Pimenta 1
PMCID: PMC4131578

Abstract

A quantitative 1H NMR method (qHNMR) was used to measure the trigonelline content in the leaves of six species of the Annona genus. The methodology employed compared the intensities of the signals at δ 9.14 (H-2) and δ 0.00, the internal standard TSP-d4. This measuring method was able to establish the concentration of trigonelline in the range from 0.67 to 10.04 mg·g−1 depending on the investigated extract. graphic file with name 13659_2013_51_Article_Fig1_HTML.jpg

Keywords: trigonelline, Annona, quantitative 1H NMR

Footnotes

This article is published with open access at Springerlink.com

References

  • [1].Blunden G, Cripps A L, Gordon S M, Mason T G, Turner C H. Bot. Mar. 1986;29:155–160. doi: 10.1515/botm.1986.29.2.155. [DOI] [Google Scholar]
  • [2].Blunden G, Patel A V. Biochem. Syst. Ecol. 2005;33:904–920. doi: 10.1016/j.bse.2005.01.005. [DOI] [Google Scholar]
  • [3].Rochfort S, Parker A J, Dunshea F R. Phytochemistry. 2008;69:299–322. doi: 10.1016/j.phytochem.2007.08.017. [DOI] [PubMed] [Google Scholar]
  • [4].Corol D I, Ravel C, Raksegi M, Bedo Z, Charmet G, Beale M H, Shewry P R, Ward J L. J. Agric. Food Chem. 2012;60:5471–5481. doi: 10.1021/jf3008794. [DOI] [PubMed] [Google Scholar]
  • [5].Sánchez-Hernández L, Castro-Puyana M, García-Ruiz C, Crego A L, Marina M L. J. Agric. Food Chem. 2010;120:921–928. doi: 10.1016/j.foodchem.2009.11.004. [DOI] [PubMed] [Google Scholar]
  • [6].Tyihak E, Sarhan A R T, Cong N T, Barna B, Kiraly Z. Plant Soil. 1988;109:285–287. doi: 10.1007/BF02202097. [DOI] [Google Scholar]
  • [7].Dini I, Tenore G C, Trimarco E, Dini A. Food Chem. 2005;98:209–213. doi: 10.1016/j.foodchem.2005.05.014. [DOI] [Google Scholar]
  • [8].Johns E. Berichte der Deutschen Chemischen Gesellschaft. 1985;18:2518–2523. doi: 10.1002/cber.188501802144. [DOI] [Google Scholar]
  • [9].Mathur M, Kamal R. Rev. Bras. Farmacogn. 2012;22:994–1001. doi: 10.1590/S0102-695X2012005000041. [DOI] [Google Scholar]
  • [10].Machado A R T, Campos V A C, Silva W R J, Campos V P, Zeri A C M, Oliveira D F. Eur. J. Plant Pathol. 2012;134:431–441. doi: 10.1007/s10658-012-0008-3. [DOI] [Google Scholar]
  • [11].Wishart D S. Trends Food Sci. Tech. 2010;19:482–493. doi: 10.1016/j.tifs.2008.03.003. [DOI] [Google Scholar]
  • [12].Barbalho M S, Alvares Goulart R, Farinazzi-Machado F M V, Souza M S S, Cincotto Bueno P C S, Guiguer E L, Araujo A C, Groppo M. Curr. Bioact. Compd. 2012;8:277–286. doi: 10.2174/157340712802762500. [DOI] [Google Scholar]
  • [13].Pieri V, Belancic A, Morales S, Stuppner H. J. Agric. Food Chem. 2011;59:4378–4384. doi: 10.1021/jf104922q. [DOI] [PubMed] [Google Scholar]
  • [14].Satheeshkumar N, Mukherjee P K, Bhadra S, Saha B P. Phytomedicine. 2010;17:292–295. doi: 10.1016/j.phymed.2009.06.006. [DOI] [PubMed] [Google Scholar]
  • [15].Kim H K, Choi Y A, Verpoorte R. Nat. Protoc. 2010;5:36–49. doi: 10.1038/nprot.2009.237. [DOI] [PubMed] [Google Scholar]

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