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. 2013 Dec 3;3(6):282–287. doi: 10.1007/s13659-013-0070-3

In vitro bone inducing effects of Lentinula edodes (shiitake) water extract on human osteoblastic cell cultures

Ashraf Saif 1,✉, Kristian Wende 2, Ulrike Lindequist 2
PMCID: PMC4131604

Abstract

The effect of Lentinula edodes water extract (LE) on two osteoblastic cell cultures (HOS 58 and Saos-2) was investigated to determine if this edible medicinal mushroom has osteoinductive properties. Activity of alkaline phosphatase and mineralization were used as indicators for the vitality and maturation of the bone cells. Cultivation of human osteosarcoma cells HOS 58 for five days in presence of a serial dilution of the aqueous extract of L. edodes (0.8 μg/mL-125 μg/mL) resulted in a significant elevation of alkaline phosphatase activity (ALP) of the cells in comparison to untreated cells. Saos-2 cells, incubated with LE (20 μg/mL) and β-glycerol phosphate (2 mM) for 21 days, displayed a 2 fold level of mineralization than cells cultured soley with the positive control, β-glycerophosphate. The obtained results clearly indicate the activity of LE as a bone inducing agent in vitro. Therefore, the shiitake mushroom (L. edodes) deserves attention as a supportive dietary treatment or nutraceutical in the case of diseases accompanied with bone disorder, such as osteoporosis, osteopenia, and late complication of diabetes. graphic file with name 13659_2013_70_Article_Fig1_HTML.jpg

Keywords: medicinal mushrooms, Lentinula edodes, osteoporosis, alkaline phosphatase, bone mineralization, shiitake

Footnotes

This article is published with open access at Springerlink.com

References

  • [1].Kaplan B, Hirsch M. Clin. Exp. Obstet. Gynecol. 2004;31:251–255. [PubMed] [Google Scholar]
  • [2].Stevenson J C. Maturitas. 2005;51:113–126. doi: 10.1016/j.maturitas.2005.01.012. [DOI] [PubMed] [Google Scholar]
  • [3].Prelevic G M, Kocjan T, Markou A. Endocrinology. 2005;30(1):27–36. [PubMed] [Google Scholar]
  • [4].Wiseman R A. J. Clin. Epidemiol. 2004;57(8):766–772. doi: 10.1016/j.jclinepi.2003.10.017. [DOI] [PubMed] [Google Scholar]
  • [5].Davison S, Davis S R. Clin. Endocrin. 2003;58:249–261. doi: 10.1046/j.1365-2265.2003.01774.x. [DOI] [PubMed] [Google Scholar]
  • [6].Huang Y H, Ye X Q. Chung Kuo Chung Hsi I Chieh Ho Tsa Chih (CHINA) 1993;13(9):522–524. [Google Scholar]
  • [7].Borchers A T, Stern J S, Hackman R M, Keen C L, Gershwin M E. Proc. Soc. Exp. Biol. Med. 1999;221:281–293. doi: 10.3181/00379727-221-44412. [DOI] [PubMed] [Google Scholar]
  • [8].Chang S T, Miles P. G. Boca Ranton. 1989. pp. 27–40. [Google Scholar]
  • [9].Breene W M. J. Food Project. 1990;53:883–894. doi: 10.4315/0362-028X-53.10.883. [DOI] [PubMed] [Google Scholar]
  • [10].Lindequist U, Niedermeyer T, Jülich W D. eCAM. 2005;2(3):285–299. doi: 10.1093/ecam/neh107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • [11].Saif A, Wende K, Lindequist U. J. Nat. Med. 2007;6:132–135. [Google Scholar]
  • [12].Niedermeyer T H J, Jira T, Lalk M, Lindequist U. Nat. Prod. Bioprospect. 2013;3:137–140. doi: 10.1007/s13659-013-0036-5. [DOI] [Google Scholar]
  • [13].Shouji N, Takada K, Fukushima K, Hirasawa M. Caries Res. 2000;34:94–98. doi: 10.1159/000016559. [DOI] [PubMed] [Google Scholar]
  • [14].Chang S T, Wasser S P. Int. J. Med. Mushrooms. 2012;14(2):95–134. doi: 10.1615/IntJMedMushr.v14.i2.10. [DOI] [PubMed] [Google Scholar]
  • [15].Jong S C, Birmingham J M. Adv. Appl. Microbiol. 1993;39:153–184. doi: 10.1016/S0065-2164(08)70595-1. [DOI] [PubMed] [Google Scholar]
  • [16].Tokita F, Shibukawa N, Yasumoto T, Kaneda T. Mushroom Sci. 1972;8:783–788. [Google Scholar]
  • [17].Suhadolnik, R. J. John Wiley and Sons, New York. 1979, 298–310.
  • [18].Kurasawa S, Sugahara T, Hayashi J. Nutr. Rep. Int. 1992;26:167–173. [Google Scholar]
  • [19].Mundy G R, Garrett R, Harris S, Chan J, Chen D, Rossini G, Boyce B, Zhao M, Gulierrez G. Science. 1999;286:1946–1949. doi: 10.1126/science.286.5446.1946. [DOI] [PubMed] [Google Scholar]
  • [20].Bauer D C, Mundy G R, Jamal S A, Black D M, Cauley J A, Harris F, Duong T, Cummings S R. J. Bone Miner. Res. 1999;14:179. doi: 10.1359/jbmr.1999.14.8.1404. [DOI] [Google Scholar]
  • [21].LaCroix A Z, Cauley J A, Jackson R, Mc Gowan J, Pettinger M, Hsia J, Chen Z, Lewis C, Bauer D C, Dangherty S, Mc Neeley S G, Passaro M. J. Bone Miner. Res. 2000;15(1):1066. [Google Scholar]
  • [22].van Staa, T. P.; Wegman, S.; de Vries, F.; Leufkens, B.; Cooper, C. 2001, 285, 1850–1855. [DOI] [PubMed]
  • [23].Lee G S, Byun H S, Yoon K H, Lee J S, Choi C K, Jeung E B. Eur. J. Nutr. 2009;48(2):75–83. doi: 10.1007/s00394-008-0763-2. [DOI] [PubMed] [Google Scholar]
  • [24].Cruz C, Gruber L. Cleve. Clin. J. Med. 2002;69(4):277–288. doi: 10.3949/ccjm.69.4.277. [DOI] [PubMed] [Google Scholar]
  • [25].Stein G S, Lian J B, Owen T A. FASEB J. 1990;4:3111–3123. doi: 10.1096/fasebj.4.13.2210157. [DOI] [PubMed] [Google Scholar]
  • [26].Perizzolo D, Lacefield W R, Brunette D M. J. Biomed. Mater. Res. 2001;56:494–503. doi: 10.1002/1097-4636(20010915)56:4<494::AID-JBM1121>3.0.CO;2-X. [DOI] [PubMed] [Google Scholar]
  • [27].zur Nieden N I, Kempka G, Ahr H J. Differentiation. 2003;71:18–27. doi: 10.1046/j.1432-0436.2003.700602.x. [DOI] [PubMed] [Google Scholar]
  • [28].Beck G R, Zerler B, Moran E J. Cell Biochem. 1998;68:269–280. doi: 10.1002/(SICI)1097-4644(19980201)68:2<269::AID-JCB13>3.0.CO;2-A. [DOI] [PubMed] [Google Scholar]
  • [29].Mosmann T. J. Immunol. Methods. 1983;65:55–63. doi: 10.1016/0022-1759(83)90303-4. [DOI] [PubMed] [Google Scholar]
  • [30].Ciapetti G, Cenni E, Pratelli L, Pizzoferrato A. Biomaterials. 1993;14:359–364. doi: 10.1016/0142-9612(93)90055-7. [DOI] [PubMed] [Google Scholar]
  • [31].Zor T, Selinger Z. Anal. Biochem. 1996;236:302–308. doi: 10.1006/abio.1996.0171. [DOI] [PubMed] [Google Scholar]
  • [32].von Kossa J. Beitr. Path. Anat. 1901;29:163–202. [Google Scholar]
  • [33].Cheung, P. C. K. Mushrooms as Functional Foods. John Wiley & Sons, Inc. Publ., Hoboken, New Jersey; p. 76.
  • [34].Cağlarirmak N. Food Chem. 2007;105:1188–1194. doi: 10.1016/j.foodchem.2007.02.021. [DOI] [Google Scholar]

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