SEROLOGIC SURVEY ON HANTAVIRUS IN BLOOD DONORS FROM THE STATE OF SANTA CATARINA, BRAZIL

Abstract

Emergent diseases such as Hantavirus Cardio-pulmonary Syndrome (HCPS) are able to create a significant impact on human populations due to their seriousness and high fatality rate. Santa Catarina, located in the South of Brazil, is the leading state for HCPS with 267 reported cases from 1999 to 2011. We present here a serological survey on hantavirus in blood donors from different cities of the state of Santa Catarina, with an IgG-ELISA using a recombinant nucleocapsid protein from Araraquara hantavirus as an antigen. In total, 314 donors from blood banks participated in the study, geographically covering the whole state. Among these, 14 individuals (4.4%) had antibodies to hantavirus: four of 50 (8% positivity) from Blumenau, four of 52 (7.6%) from Joinville, three of 50 (6%) from Florianópolis, two of 50 (4%) from Chapecó and one of 35 (2.8%) from Joaçaba. It is possible that hantaviruses are circulating across almost the whole state, with important epidemiological implications. Considering that the seropositive blood donors are healthy individuals, it is possible that hantaviruses may be causing unrecognized infections, which are either asymptomatic or clinically nonspecific, in addition to HCPS. It is also possible that more than one hantavirus type could be circulating in this region, causing mostly benign infections.

INTRODUCTION

Emergent diseases that are commonly caused by zoonotic microbes, such as Hantavirus Cardio-Pulmonary Syndrome (HCPS), cause a significant impact on human populations due to their seriousness and high case fatality rates. Hantaviruses are 100 to 120 nm in diameter tri-segmented RNA viruses of the Bunyaviridae family. The large (L) RNA segment codes for a RNA polymerase, the medium (M) segment encodes the glycoproteins of viral surface (Gn and Gc), and the small segment (S) codes for the nucleocapsid (N) protein, which is an important viral antigen and induces antibody production after infection³. Small mammals, especially rodents, are the natural reservoirs of hantavirus. The transmission of these viruses to man occurs by the inhalation of contaminated aerosols containing excreta of infected rodents. In the Americas, hantaviruses are the cause of HCPS, which has been recognized since 1993⁵. In Brazil, about 1600 HCPS cases were reported from 1993 to 2013 with a 40% case fatality rate (Source: SVC/MS). Five hantaviruses are known to cause HCPS in Brazil: Anajatuba, Araraquara (ARAV), Castelo dos Sonhos (CSV), Juquitiba (JUQV), and Laguna Negra-like virus³. Some factors could be highlighted as contributors to the emergence of hantaviruses, including ecological degradation with deforestation for poorly planned urban expansion, intensive agriculture and cattle raising.

In recent years, a recombinant N protein of ARAV was developed, which has been used as an antigen in an ELISA for the diagnosis of hantavirus infections⁴. This ELISA is considered an important weapon for serologic surveillance of hantavirus infections in humans and rodents in Brazil and has been used in many epidemiological studies.

Santa Catarina, located in the South of Brazil, is the leading state on HCPS reported cases (Source: SVC/MS). However, the prevalence and other factors related to hantavirus infection and HCPS are not well known in the state. We present here a serologic survey of hantavirus in blood donors from different cities of the state of Santa Catarina, by using an IgG-ELISA with the N recombinant protein of ARAV.

MATERIAL AND METHODS

Participants and serum samples: In total, 317 blood donors from both genders (101 men and 216 women), from ages of 18 to 68 years participated in this serological survey. From February through March 2012, these participants donated blood and those that signed a consent form were enrolled in the study. In order to geographically represent the whole state of Santa Catarina, 40 to 50 participants from each of the blood banks in the state's larger cities were included in the study: Blumenau city in the Itajaí Valley; Joinville, in the north of the state; Florianópolis city, in the eastern coast; Criciúma city, in the south of the state; Lages city, in the central mountains plateau; and Chapecó city and Joaçaba city, in the west of the state. A 5 mL blood sample was collected in Vacutainer™ (Becton Dickinson, Franklin Lakes, NJ, USA) from each participant by an officer of the blood bank, together with samples used for routine serologic tests. The blood was centrifuged at 3,000 rpm for five min; the serum was separated, carried to the laboratory on ice and stored at -20 °C until use. This Research Project was approved by the Ethics Committee for Human Research of the Regional University of Blumenau (Protocol n° 155/10), in accordance with the Helsinki Declaration of 1975 as revised in 1983 and the International Ethical Guidelines for Biomedical Research Involving Human Subjects (CIOMS/OMS 1982 & 1993).

IgG-ELISA for hantavirus: The serum samples were tested in duplicate by an anti-hantavirus IgG-ELISA using the N recombinant protein of ARAV as antigen. Tests were performed as previously described⁴. Briefly, polystyrene microtiter plates (polystyrene high biding, Corning) were coated overnight in a wet chamber at 4 °C with 2 µg/mL of ARAV N protein, or control antigen. All incubations were conducted at 37 °C for one hour and plates were washed six times with a wash buffer (phosphate-buffered saline [PBS]–0.1% Tween 20) between each step. Firstly, 150 µL of a blocking solution containing 10% skimmed milk in powder in [PBS]–0.1% Tween 20 was added to the wells. All serum samples were diluted 1:100 in dilution buffer (5% skimmed milk powder in PBS-Tween-20), as were the positive and the negative control sera, and 50 µL was added to the antigen-coated wells. Peroxidase-labeled affinity-purified goat anti-human IgG Fc antibody (50 µL/well) was added and specific antibody binding was detected by the addition of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) substrate (100 µL/well), with the absorbance measured at 405 nm. Values were expressed as the optical density (OD) obtained with ARAV N antigen minus the OD values for the control antigens. The cut-off value of the test was determined by the mean plus three standard deviations of the ODs obtained for at least four negative-control serum samples, plus three standard deviations of the mean.

RESULTS AND DISCUSSION

This serologic survey of the hantavirus included 314 participants from blood banks geographically covering the whole state of Santa Catarina (Fig. 1). Of these, 14 individuals (4.4%) had antibodies to hantavirus: four of 50 (8% positivity) from Blumenau city, four of 52 (7.6%) from Joinville city, three of 50 (6%) from Florianópolis city, two of 50 (4%) from Chapecó city and one of 35 (2.8%) from Joaçaba city. No seropositive results were observed among the 42 participants of Criciúma city or 35 participants from Lages city.

Fig. 1 -. Map of the South of Brazil showing the city location of blood banks of the state of Santa Catarina that provided participants for the Hantavirus serological survey. Source: http://maps.google.com/ .

The levels of antibody to hantavirus in blood donors in any state could provide information on the circulation of these viruses in the entire state, including geographical areas where HCPS has not been reported.

Hantavirus infections causing HCPS are well known in the state of Santa Catarina, as 267 confirmed cases were reported from 1999 through December 2011. Curiously, only a few HCPS cases have been reported on the eastern coast in the state Capital Florianópolis, and in the north-eastern region of Joinville². In the present study, more than 50% of individuals that were seropositive for hantavirus were from these regions. Antibodies to hantavirus were not detected in participants from the South of Santa Catarina (Criciúma city). However, a study in a rural population next to Criciúma city showed a 2.3% seroprevalence to hantavirus and all seropositive individuals referred a previous severe pneumonia that could have been HCPS⁶.

This study shows that 4.4% of blood donors are infected by hantavirus in most of the Santa Catarina territory. Considering that blood donors are commonly local inhabitants, it is possible that hantaviruses are circulating across practically all of the state. Of note, a study including 340 individuals inhabiting two cities in the western border of the state of Santa Catarina with Argentina, found 3.5% of individuals to be seropositive for hantavirus⁷. It might be worth noting that hantavirus infection is usually acquired by contact with wild rodents, often in the rural areas, and not in the urban environment. Blood Banks as the ones evaluated in the present study usually receive donors from the whole region they serve, or even from abroad. Additional studies evaluating the precise origin of the seropositive donors might be of interest to determine the possible site of origin of hantavirus exposure.

Considering that the seropositive blood donors are healthy individuals, it is possible that hantaviruses may be causing unrecognized infections, which are either asymptomatic or clinically nonspecific, in addition to HCPS. It is also possible that more than one hantavirus type could be circulating in this region, causing mostly benign infections. Once immune activation has been associated with HCPS pathogenesis, it is possible that some polymorphisms in genes involved in immune response may affect the development of HCPS¹. Further studies are necessary in order to explain unrecognized infections by hantaviruses.

Funding Statement

Ministry of Health, Brazil; Ministry of Science and Technology; State Secretary of Health of Santa Catarina; Fundação de Amparo à Pesquisa e Inovação do Estado de Santa Catarina; University of Blumenau; MS/ CNPq/FAPESC/SES PPSUS 003/2010 research grant nº 3.747-2011-8.