Figure 3.

Channeling assays with increasing concentrations of D779Y (A) and D779W (B). NADH formation was monitored using fluorescence by exciting at 340 nm and recording the emission at 460 nm. Assays were performed with wild-type BjPutA (0.187 μM) and increasing concentrations of mutants (0.187–1.87 μM) in 50 mM potassium phosphate (pH 7.5, 25 mM NaCl, 10 mM MgCl₂) containing 40 mM proline, 100 μM CoQ₁, and 200 μM NAD⁺.