VOLUME 287 (2012) PAGES 33198–33214
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The curcumin nanoparticle binding assay described failed to indicate that 1 ml of chitosan (5 mg/ml stock at pH 5) was combined with PEG, curcumin, and phosphate-buffered saline in the first step and that hydrolyzed tetramethylorthosilicate (TMOS) was used, not trimethoxysilane. This protocol was based on the platform developed by Friedman et al. (Friedman, A. J., Han, G., Navati, M. S., Chacko, M., Gunther, L., Alfieri, A., and Friedman, J. M. (2008) Sustained release nitric oxide releasing nanoparticles: characterization of a novel delivery platform based on nitrite containing hydrogel/glass composites. Nitric Oxide 19, 12–20).
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The curcumin nanoparticles described in Fig. 5B were developed and synthesized by Mahantesh H. Navati and Joel M. Friedman (Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY) and Adam J. Friedman (Division of Dermatology, Department of Medicine, Albert Einstein College of Medicine, Bronx, NY). Madiha Sewani also assisted in synthesizing the nanoparticles.