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. 1993 Mar;12(3):1005–1012. doi: 10.1002/j.1460-2075.1993.tb05741.x

UV-induced activation of AP-1 involves obligatory extranuclear steps including Raf-1 kinase.

A Radler-Pohl 1, C Sachsenmaier 1, S Gebel 1, H P Auer 1, J T Bruder 1, U Rapp 1, P Angel 1, H J Rahmsdorf 1, P Herrlich 1
PMCID: PMC413301  PMID: 8384549

Abstract

Irradiation of cells with ultraviolet light (UV) leads to modifications of c-Jun resembling those elicited by phorbol esters or oncogenes, and to enhanced transcription of AP-1-dependent genes. The UV-induced signal also triggers activation of Raf-1 and MAP-2 kinases. A dominant-negative Raf-1 kinase mutant strongly interferes with both phorbol ester and UV-induced AP-1 activation, indicating obligatory involvement of identical components in cytoplasmic signal transduction. Thus, from a presumably nuclear site of energy absorption, a signal needs to be transmitted to the cytoplasm in order to achieve activation of a nuclear transcription factor. Further, signals elicited from different primary sites merge prior to or at the level of activation of Raf-1 kinase.

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Selected References

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