Figure 6. Recruitment of coilin, 53BP1 and γH2AX to UVA-induced DNA lesions. (A) (a) Recruitment of coilin (green) and 53BP1 (red) to locally induced DNA lesions without BrdU pre-sensitization. (b) Increased levels of coilin (green) and γH2AX (red) were observed in UVA-induced DNA lesions without BrdU pre-sensitization. (B) Nuclear localization patterns of coilin (green) and 53BP1 (red), or coilin (green) and γH2AX (red) in HeLa cells irradiated with 5 Gy of γ-rays and in control non-irradiated cells; cells were analyzed 0.5, 2, 6, and 24 h after γ-irradiation. (C) HeLa cells were irradiated with 5 Gy of γ-rays and then with UVA in selected regions of interest denoted by the white box. Coilin (green) and 53BP1 (red) were recruited to DNA lesions and γ-irradiation-induced foci appeared (white arrow).