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Robust H2BGFP induction kinetics of Dox-induced double transgenic mice. Double transgenic mice were treated with dox for indicated amount of time, tissue was analyzed by: Top, qRT-PCR using primers specific for H2BGFP expression which was normalized to rtTA and GAPDH mRNA level. Bottom, Western blot analyses of cell lysate obtained from treated ventral prostates using antibodies against H2BGFP and β-Actin showing longer time points.
