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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Hypertension. 2014 Jun 9;64(3):610–618. doi: 10.1161/HYPERTENSIONAHA.114.03407

Figure 6. Effect of CpG methylation at Sp1-380 site on KCNMB1 promoter activity.

Figure 6

Full length wild-type (WT-KCNMB1), methylated CpG at Sp1-380 (Sp1/ERα-M), unmethylated CpG at Sp1-380 (Sp1/ERα-UM), and a dual insertion of SacII and SmaI sites (KCNMB1-SacII-SmaI) constructs were transfected into uterine arterial smooth muscle cells. Firefly and Renilla reniformis luciferase activities were measured in a luminometer using a dual-luciferase reporter assay system. Data are mean ± SEM. * P < 0.05, versus KCNMB1-SacII-SmaI. n=5