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. Author manuscript; available in PMC: 2015 Aug 14.
Published in final edited form as: Cell. 2014 Aug 14;158(4):849–860. doi: 10.1016/j.cell.2014.05.050

Figure 1. Reactivation of the silenced βh1-globin gene in adult erythroid cells by the tethered Ldb1 SA domain.

Figure 1

(A) Experimental strategy. In normal adult erythroid cells, Ldb1 is part of a multi-protein complex at the LCR and the β-major promoter and contributes to LCR- β-major contacts. Zinc finger mediated tethering of the SA domain of Ldb1 to the βh1-globin promoter might juxtapose the βh1-globin promoter to the LCR at the expense of the βmaj-LCR interaction. (B) Anti-Flag ChIP in G1E cells expressing βh1-SA. (C) mRNA levels of βh1-globin in G1E cells (left) or G1E cells expressing activated GATA1-ER (G1E+GATA1, right) transduced with empty vector, or vector expressing SA fused to LCR-specific zinc finger (L-SA) or βh1-specific zinc finger (βh1-SA). (D) (left) βh1 mRNA levels plotted as percentage of total β-globin transcripts; (right) β-major expression in G1E+GATA1 cells transduced with indicated constructs. Expression is normalized to β-actin. Error bars denote SD. N=3. See also Figure S1.