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. 1995 Aug 1;92(16):7510–7514. doi: 10.1073/pnas.92.16.7510

Operator binding by lambda repressor heterodimers with one or two N-terminal arms.

Y I Kim 1, J C Hu 1
PMCID: PMC41369  PMID: 7638221

Abstract

The first 6 amino acids (NH2-Ser1-Thr2-Lys3-Lys4-Lys5-Pro6) of bacteriophage lambda cI repressor form a flexible arm that wraps around the operator DNA. Homodimeric lambda repressor has two arms. To determine whether both arms are necessary or only one arm is sufficient for operator binding, we constructed heterodimeric repressors with two, one, or no arms by fusing the DNA binding domain of lambda repressor to leucine zippers from Fos and Jun. Although only one arm is visible in the cocrystal structure of the N-domain-operator complex, our results indicate that both arms are required for optimal operator binding and normal site discrimination.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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