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. 2014 Jul 29;143(1):96–108. doi: 10.1111/imm.12292

Figure 2.

Figure 2

Generation of multi-tumour antigen-specific CD4+ T cells with interferon-γ (IFN-γ) matured dendritic cells (DC). Representative intracellular flow cytometry plots for IFN-γ, tumour necrosis factor-α (TNF-α) and interleukin-2 (IL-2) secretion by CD4+ T cells following the generation of large numbers of antigen-specific T cells specific for WT1, PR3, AK1, and PRAME. Autologous lymphocytes were primed with IFN-γ-matured monocyte-derived DC (moDC) pulsed with the various overlapping peptide pools individually or all together (Mix) and grown for 2 weeks in culture media supplemented with IL-4 1000 IU/ml, IL-7 (10 ng/ml) and IL-2 (10 IU/ml). NP = T cells grown with moDC with no peptides. All plots gated on live vivid−, CD3+, and CD4+ T cells. Data are representative of five healthy donors.