Glucose tolerance was attenuated and response to insulin tolerance test differed after chronic feeding in female RIIαKO mice HFD feeding. GTT (A) and ITT (B) in female WT and RIIαKO after CD and HFD feeding. Intraperitoneal glucose injection (2 g/kg) and insulin (HumulinR) (0.75 U/kg) was administered, respectively, for GTT and ITT. Blood glucose was measured at baseline and 15, 30, 60, and 120 minutes after glucose injection and at baseline and 15, 30, 45, and 60 minutes after insulin injection. Values are mean ± SEM. *, P < .05.