Table 4.

PKA Subunit mRNA and Protein Expression in Gonadal AT of WT and RIIαKO Mice After 14 Weeks of ad Libitum Feeding With CD or HFD

	RIα	RIIα	RIIβ	Cα	Cβ
mRNA expression of PKA subunits (qPCR)
CD
WT (n = 8)	1.07 ± 0.14	0.97 ± 0.15	1.40 ± 0.37	1.21 ± 0.27	1.06 ± 0.14
Mutant (n = 7)	1.22 ± 0.12	0.30 ± 0.22	2.73 ± 0.51	2.18 ± 0.29	1.27 ± 0.09
HFD
WT (n = 8)	1.26 ± 0.08	1.70 ± 0.27	2.60 ± 0.18	1.67 ± 0.13	1.49 ± 0.12
Mutant (n = 8)	1.34 ± 0.07	0.24 ± 0.04	3.19 ± 0.36	1.72 ± 0.10	1.52 ± 0.12
	P = .30	P < .001	P = .017	CD, P = .031; HFD, P = .80a	P = .32
Protein expression of PKA subunits (Western blot)
CD
WT (n = 5)	1.47 ± 0.48	1.56 ± 0.41	1.22 ± 0.34	0.29 ± 0.09	0.13 ± 0.04
Mutant (n = 2)	1.36 ± 0.10	0.13 ± 0.10	1.89 ± 1.05	0.96 ± 0.07	0.08 ± 0.01
HFD
WT (n = 5)	0.32 ± 0.08	1.17 ± 0.27	0.30 ± 0.21	1.65 ± 0.33	0.19 ± 0.03
Mutant (n = 2)	0.50 ± 0.30	0.22 ± 0.05	0.46 ± 0.37	0.40 ± 0.30	0.08 ± 0.00
	P = .94	P = .012	P = .45	CD, P = .030; HFD, P = .083a	P = .054

Abbreviation: qPCR, quantitative PCR. mRNA expression was represented as fold change (2^−ΔΔCT) based on WT mice on CD. Protein expression was calculated as the ratio of protein of interest to housekeeper. Data for female mice are shown. P values derive from multivariable ANCOVA adjusting for diet; values are mean ± SEM.

^aReported separately for each diet due to statistically significant interaction with genotype.