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. 1978 Jan;19(1):66–70. doi: 10.1128/iai.19.1.66-70.1978

Purification of Pseudomonas aeruginosa exotoxin by affinity chromatography.

N S Taylor, M Pollack
PMCID: PMC414049  PMID: 415008

Abstract

Pseudomonas aeruginosa exotoxin A was purified by affinity chromatography from culture supernatants by elution of toxin from antitoxin immunoglobulin G-Sepharose 4B with 3 M NaSCN. The purity, toxicity, and enzymatic activity of exotoxin obtained were comparable to those of toxin purified by previously reported multiple-step procedures.

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Selected References

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