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. 1978 Jan;19(1):116–122. doi: 10.1128/iai.19.1.116-122.1978

Production of Extracellular and Cell-Associated Glucosyltransferase Activity by Streptococcus mutans During Growth on Various Carbon Sources

William M Janda 1, Howard K Kuramitsu 1
PMCID: PMC414056  PMID: 624585

Abstract

The production of extracellular and cell-associated glucosyltransferase activity by Streptococcus mutans strain GS-5 was examined during growth on various carbon sources in a chemically defined medium. S. mutans cells produced glucosyltransferase activity only during logarithmic growth when glucose, fructose, mannitol, or sorbitol was the sole carbon source. Cells growing on mannitol or sorbitol produced approximately half as much extracellular glucosyltransferase activity as cells growing on glucose, although the proportions of the glucosyltransferase activity capable of synthesizing insoluble glucans were similar. Cells growing on fructose produced slightly more extracellular glucosyltransferase activity than cells grown on glucose, yet the proportion of the glucosyltransferase activity capable of synthesizing insoluble glucans was again similar to glucose cultures. S. mutans cells growing in the presence of both glucose and mannitol displayed diauxic growth and initial preferential utilization of glucose. Glucosyltransferase enzyme production occurred only during the phases of cell growth in the presence of the two carbon sources. The cell-associated glucosyltransferase activities of glucose-, fructose-, mannitol-, and sorbitol-grown cells were relatively low, yet all the cells were capable of adherence to glass in the presence of sucrose. When glucose-containing cultures of S. mutans were supplemented with sucrose, extracellular glucosyltransferase activity first became cell associated and then appeared to become inactivated, presumably due to the accumulation of insoluble glucans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown A. T., Bowles R. D. Polyol metabolism by a caries-conducive Streptococcus: purification and properties of a nicotinamide adenine dinucleotide-dependent mannitol-1-phosphate dehydrogenase. Infect Immun. 1977 Apr;16(1):163–173. doi: 10.1128/iai.16.1.163-173.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown A. T., Wittenberger C. L. Mannitol and sorbitol catabolism in Streptococcus mutans. Arch Oral Biol. 1973 Jan;18(1):117–126. doi: 10.1016/0003-9969(73)90026-5. [DOI] [PubMed] [Google Scholar]
  3. Chassy B. M., Beall J. R., Bielawski R. M., Porter E. V., Donkersloot J. A. Occurrence and distribution of sucrose-metabolizing enzymes in oral streptococci. Infect Immun. 1976 Aug;14(2):408–415. doi: 10.1128/iai.14.2.408-415.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Daneo-Moore L., Terleckyj B., Shockman G. D. Analysis of growth rate in sucrose-supplemented cultures of Streptococcus mutans. Infect Immun. 1975 Nov;12(5):1195–1205. doi: 10.1128/iai.12.5.1195-1205.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fukui K., Fukui Y., Moriyama T. Purification and properties of dextransucrase and invertase from Streptococcus mutans. J Bacteriol. 1974 Jun;118(3):796–804. doi: 10.1128/jb.118.3.796-804.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Germaine G. R., Schachtele C. F. Streptococcus mutans dextransucrase: mode of interaction with high-molecular-weight dextran and role in cellular aggregation. Infect Immun. 1976 Feb;13(2):365–372. doi: 10.1128/iai.13.2.365-372.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gibbons R. J., Nygaard M. Synthesis of insoluble dextran and its significance in the formation of gelatinous deposits by plaque-forming streptococci. Arch Oral Biol. 1968 Oct;13(10):1249–1262. doi: 10.1016/0003-9969(68)90081-2. [DOI] [PubMed] [Google Scholar]
  8. Guggenheim B., Newbrun E. Extracellular glucosyltransferase activity of an HS strain of Streptococcus mutans. Helv Odontol Acta. 1969 Oct;13(2):84–97. [PubMed] [Google Scholar]
  9. Guggenheim B. Streptococci of dental plaques. Caries Res. 1968;2(2):147–163. doi: 10.1159/000259553. [DOI] [PubMed] [Google Scholar]
  10. Janda W. M., Kuramitsu H. K. Properties of a variant of Streptococcus mutans altered in its ability to interact with glucans. Infect Immun. 1977 May;16(2):575–586. doi: 10.1128/iai.16.2.575-586.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Janda W. M., Kuramitsu H. K. Regulation and extracellular glucosyltransferase production and the relationship between extracellular and cell-associated activities in Streptococcus mutans. Infect Immun. 1976 Jul;14(1):191–202. doi: 10.1128/iai.14.1.191-202.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kuramitsu H. K. Characterization of cell-associated dextransucrase activity from glucose-grown cells of Streptococcus mutans. Infect Immun. 1974 Jul;10(1):227–235. doi: 10.1128/iai.10.1.227-235.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kuramitsu H. K. Characterization of extracellular glucosyltransferase activity of Steptococcus mutans. Infect Immun. 1975 Oct;12(4):738–749. doi: 10.1128/iai.12.4.738-749.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kuramitsu H. K. Properties of a mutant of Streptococcus mutans altered in glucosyltransferase activity. Infect Immun. 1976 Feb;13(2):345–353. doi: 10.1128/iai.13.2.345-353.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Maryanski J. H., Wittenberger C. L. Mannitol transport in Streptococcus mutans. J Bacteriol. 1975 Dec;124(3):1475–1481. doi: 10.1128/jb.124.3.1475-1481.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. McCabe M. M., Smith E. E. Origin of the cell-associated dextransucrase of Streptococcus mutans. Infect Immun. 1973 Jun;7(6):829–838. doi: 10.1128/iai.7.6.829-838.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mukasa H., Slade H. D. Mechanism of adherence of Streptococcus mutans to smooth surfaces. I. Roles of insoluble dextran-levan synthetase enzymes and cell wall polysaccharide antigen in plaque formation. Infect Immun. 1973 Oct;8(4):555–562. doi: 10.1128/iai.8.4.555-562.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mukasa H., Slade H. D. Mechanism of adherence of Streptococcus mutans to smooth surfaces. II. Nature of the binding site and the adsorption of dextran-levan synthetase enzymes on the cell-wall surface of the streptococcus. Infect Immun. 1974 Feb;9(2):419–429. doi: 10.1128/iai.9.2.419-429.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mukasa H., Slade H. D. Mechanism of the Adherence of Streptococcus mutans to Smooth Surfaces III. Purification and Properties of the Enzyme Complex Responsible for Adherence. Infect Immun. 1974 Nov;10(5):1135–1145. doi: 10.1128/iai.10.5.1135-1145.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schachtele C. F., Harlander S. K., Germaine G. R. Streptococcus mutans dextransucrase: availability of disaggregated enzyme after growth in a chemically defined medium. Infect Immun. 1976 May;13(5):1522–1524. doi: 10.1128/iai.13.5.1522-1524.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Scherp H. W. Dental caries: prospects for prevention. Science. 1971 Sep 24;173(4003):1199–1205. doi: 10.1126/science.173.4003.1199. [DOI] [PubMed] [Google Scholar]
  22. Spinell D. M., Gibbons R. J. Influence of culture medium on the glucosyl transferase- and dextran-binding capacity of Streptococcus mutans 6715 cells. Infect Immun. 1974 Dec;10(6):1448–1451. doi: 10.1128/iai.10.6.1448-1451.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tanzer J. M., Chassy B. M., Krichevsky M. I. Sucrose metabolism by Streptococcus mutans, SL-I. Biochim Biophys Acta. 1971 Feb 28;261(2):379–387. doi: 10.1016/0304-4165(72)90062-1. [DOI] [PubMed] [Google Scholar]
  24. Terleckyj B., Willett N. P., Shockman G. D. Growth of several cariogenic strains of oral streptococci in a chemically defined medium. Infect Immun. 1975 Apr;11(4):649–655. doi: 10.1128/iai.11.4.649-655.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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