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. 1978 Jan;19(1):296–303. doi: 10.1128/iai.19.1.296-303.1978

Activation of the alternate complement pathway by peptidoglycan from streptococcal cell wall.

J Greenblatt, R J Boackle, J H Schwab
PMCID: PMC414080  PMID: 415005

Abstract

Activation of the alternate complement pathway in human serum by several bacterial components was compared. Peptidoglycan from group A streptococcal cell walls was the most active material, on a weight basis, followed by cell walls, protoplast membranes, and whole cells. The group-specific carbohydrate was inactive. Treatment of peptidoglycan with low concentrations of lysozyme or short periods of sonic treatment enhanced complement activation. High concentrations of lysozyme or extended sonic treatment of peptidoglycan destroyed or greatly reduced the capacity to activate complement. Lysozyme treatment of group A streptococcal cell walls or lipopolysaccharide had no measurable effect. Activation of the alternate complement pathway by group D streptococcal cell walls was destroyed by lysozyme. Activity of peptidoglycan was not inhibited by N-acetyl glucosamine, N-acetyl muramic acid, or D-alanine-D-alanine. Conversion of C3 and factored B by peptidoglycan was shown to occur by immunoelectrophoresis and crossed immunoelectrophoresis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdulla E. M., Schwab J. H. Biological properties of streptococcal cell-wall particles. 3. Dermonecrotic reaction to cell-wall mucopeptides. J Bacteriol. 1966 Jan;91(1):374–383. doi: 10.1128/jb.91.1.374-383.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boackle R. J., Pruitt K. M., Mestecky J. The interactions of human complement with interfacially aggregated preparations of human secretory IgA. Immunochemistry. 1974 Sep;11(9):543–548. doi: 10.1016/0019-2791(74)90245-6. [DOI] [PubMed] [Google Scholar]
  3. Bokisch V. A. Interaction of peptidoglycans with anti-IgGs and with complement. Z Immunitatsforsch Exp Klin Immunol. 1975 Jul;149(2-4):320–330. [PubMed] [Google Scholar]
  4. Burger R., Hadding U., Schorlemmer H. U., Brade V., Bitter-Suermann D. Dextran sulphate: a synthetic activator of C3 via the alternative pathway. I. Influence of molecular size and degree of sulphation on the activation potency. Immunology. 1975 Sep;29(3):549–554. [PMC free article] [PubMed] [Google Scholar]
  5. Dhingra R. K., Williams R. C., Jr, Reed W. P. Effects of pneumococcal mucopeptide and capsular polysaccharide on phagocytosis. Infect Immun. 1977 Jan;15(1):169–174. doi: 10.1128/iai.15.1.169-174.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dukor P., Schumann G., Gisler R. H., Dierich M., König W., Hadding U., Bitter-Suermann D. Complement-dependent B-cell activation by cobra venom factor and other mitogens? J Exp Med. 1974 Feb 1;139(2):337–354. doi: 10.1084/jem.139.2.337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fine D. P., Marney S. R., Jr, Colley D. G., Sergent J. S., Des Prez R. M. C3 shunt activation in human serum chelated with EGTA. J Immunol. 1972 Oct;109(4):807–809. [PubMed] [Google Scholar]
  8. Gewurz H., Shin H. S., Mergenhagen S. E. Interactions of the complement system with endotoxic lipopolysaccharide: consumption of each of the six terminal complement components. J Exp Med. 1968 Nov 1;128(5):1049–1057. doi: 10.1084/jem.128.5.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Glick A. D., Ranhand J. M., Cole R. M. Degradation of group A streptococcal cell walls by egg-white lysozyme and human lysosomal enzymes. Infect Immun. 1972 Sep;6(3):403–413. doi: 10.1128/iai.6.3.403-413.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KRAUSE R. M., MCCARTY M. Studies on the chemical structure of the streptococcal cell wall. I. The identification of a mucopeptide in the cell walls of groups A and A-variant streptococci. J Exp Med. 1961 Jul 1;114:127–140. doi: 10.1084/jem.114.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LAURELL C. B. ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS. Anal Biochem. 1965 Feb;10:358–361. doi: 10.1016/0003-2697(65)90278-2. [DOI] [PubMed] [Google Scholar]
  12. Platts-Mills T. A., Ishizaka K. Activation of the alternate pathway of human complements by rabbit cells. J Immunol. 1974 Jul;113(1):348–358. [PubMed] [Google Scholar]
  13. SCHEIDEGGER J. J. Une micro-méthode de l'immuno-electrophorèse. Int Arch Allergy Appl Immunol. 1955;7(2):103–110. [PubMed] [Google Scholar]
  14. Schleifer K. H., Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev. 1972 Dec;36(4):407–477. doi: 10.1128/br.36.4.407-477.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schreiber R. D., Götze O., Müller-Eberhard H. J. Alternative pathway of complement: demonstration and characterization of initiating factor and its properdin-independent function. J Exp Med. 1976 Oct 1;144(4):1062–1075. doi: 10.1084/jem.144.4.1062. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Schwab J. H., Brown R. R. Heart autoantibodies in mice: genetic, bacterial, and age determinants. J Immunol. 1973 Sep;111(3):682–690. [PubMed] [Google Scholar]
  17. Tauber J. W., Polley M. J., Zabriskie J. B. Nonspecific complement activation by streptococcal structures. II. Properdin-independent initiation of the alternate pathway. J Exp Med. 1976 Jun 1;143(6):1352–1366. doi: 10.1084/jem.143.6.1352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Winkelstein J. A., Tomasz A. Activation of the alternative pathway by pneumococcal cell walls. J Immunol. 1977 Feb;118(2):451–454. [PubMed] [Google Scholar]
  19. da Silva W. D., Eisele J. W., Lepow I. H. Complement as a mediator of inflammation. 3. Purification of the activity with anaphylatoxin properties generated by interaction of the first four components of complement and its identification as a cleavage product of C'3. J Exp Med. 1967 Dec 1;126(6):1027–1048. doi: 10.1084/jem.126.6.1027. [DOI] [PMC free article] [PubMed] [Google Scholar]

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