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. 1978 Feb;19(2):347–352. doi: 10.1128/iai.19.2.347-352.1978

Solid-phase radioimmunoassay method for determination of Escherichia coli enterotoxin.

M Ceska, F Grossmüller, F Effenberger
PMCID: PMC414088  PMID: 75844

Abstract

The development of a solid-phase radioimmunoassay procedure for the determination of Escherichia coli enterotoxin(s) is described. Radioiodinated E. coli enterotoxin with about three radioiodine atoms per toxin molecule is, by the criterion of electrophoresis, identical to the unlabeled toxin. Goat anti-E. coli-enterotoxin antibody was coupled to polystyrene tubes and served as a solified toxin binder in the reported procedure. Various conditions necessary for the optimization and standardization of the solid-phase method were established. With the help of this technique it was possible to determine E. coli enterotoxin released from a porcine E. coli strain into culture medium.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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