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. 1978 Feb;19(2):570–574. doi: 10.1128/iai.19.2.570-574.1978

Production of antibodies to human interferons in mice.

B J Dalton, C A Ogburn, K Paucker
PMCID: PMC414121  PMID: 631890

Abstract

Neutralizing antibodies were raised in mice that had been inoculated repeatedly with moderate quantities of human leukocyte interferon highly purified by affinity chromatography on immobilized anti-interferon globulins. Interferon preparations of lesser purity sensitized the mice to subsequent inoculations of interferon and almost invariably caused death before anti-interferon titers developed. Antibody-purified interferon stabilized by sodium dodecyl sulfate was a superior antigen to interferon that had received mouse serum albumin as an additive. The amount of antibody could be augmented by experimental induction of ascites. The antibodies specifically neutralized leukocyte and lymphoblastoid interferons but not those interferons obtained cultures of human foreskin fibroblasts, embryonic kidney cells, and amnion cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anfinsen C. B., Bose S., Corley L., Gurari-Rotman D. Partial purification of human interferon by affinity chromatography. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3139–3142. doi: 10.1073/pnas.71.8.3139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Armstrong J. A. Semi-micro, dye-binding assay for rabbit interferon. Appl Microbiol. 1971 Apr;21(4):723–725. doi: 10.1128/am.21.4.723-725.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berg K., Ogburn C. A., Paucker K., Mogensen K. E., Cantell K. Affinity chromatography of human leukocyte and diploid cell interferons on sepharose-bound antibodies. J Immunol. 1975 Feb;114(2 Pt 1):640–644. [PubMed] [Google Scholar]
  4. Cantell K., Hirvonen S., Mogensen K. E., Pyhälä L. Human leukocyte interferon: production, purification, stability, and animal experiments. In Vitro Monogr. 1974;(3):35–38. [PubMed] [Google Scholar]
  5. Cavalieri R. L., Havell E. A., Vilcek J., Pestka S. Synthesis of human interferon by Xenopus laevis oocytes: two structural genes for interferons in human cells. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3287–3291. doi: 10.1073/pnas.74.8.3287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fauconnier B. Production de sérums anti-interféron de cellules L de souris chez le lapin. Ann Inst Pasteur (Paris) 1967 Nov;113(5):757–769. [PubMed] [Google Scholar]
  7. German A., Quéro A. M., Poindron P. Essai de purification d'un interféron de poulet en vue de l'obtention d'anticorps spécifiques. Ann Pharm Fr. 1969 Feb;27(2):103–113. [PubMed] [Google Scholar]
  8. Gresser I., Fontaine D., Coppey J., Falcoff R., Falcoff E. Interferon and murine leukemia. II. Factors related to the inhibitory effect of interferon preparations on development of Friend leukemia in mice. Proc Soc Exp Biol Med. 1967 Jan;124(1):91–94. doi: 10.3181/00379727-124-31673. [DOI] [PubMed] [Google Scholar]
  9. Gresser I., Tovey M. G., Bandu M. E., Maury C., Brouty-Boyé D. Role of interferon in the pathogenesis of virus diseases in mice as demonstrated by the use of anti-interferon serum. I. Rapid evolution of encephalomyocarditis virus infection. J Exp Med. 1976 Nov 2;144(5):1305–1315. doi: 10.1084/jem.144.5.1305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hajnická V., Fuchsberger N., Borecký L. Affinity chromatography of mouse interferon: a modified purification procedure utilizing specifically purified antibodies. Acta Virol. 1976 Aug;20(4):326–333. [PubMed] [Google Scholar]
  11. Havell E. A., Berman B., Ogburn C. A., Berg K., Paucker K., Vilcek J. Two antigenically distinct species of human interferon. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2185–2187. doi: 10.1073/pnas.72.6.2185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Havell E. A., Vilcek J. Production of high-titered interferon in cultures of human diploid cells. Antimicrob Agents Chemother. 1972 Dec;2(6):476–484. doi: 10.1128/aac.2.6.476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Inglot A. D., Chudzio T. A bioassay employing polyethylene glycol for measuring neutralization of interferon by specific antibodies. J Immunol Methods. 1976;13(1):63–69. doi: 10.1016/0022-1759(76)90187-3. [DOI] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Levy-Koenig R. E., Mundy M. J., Paucker K. Immunology of interferons. I. Immune response to protective and nonprotective interferons. J Immunol. 1970 Apr;104(4):785–790. [PubMed] [Google Scholar]
  16. MUNOZ J. Production in mice of large volumes of ascites fluid containing antibodies. Proc Soc Exp Biol Med. 1957 Aug-Sep;95(4):757–759. doi: 10.3181/00379727-95-23355. [DOI] [PubMed] [Google Scholar]
  17. Mogensen K. E., Cantell K. Human leukocyte interferon: a role for disulphide bonds. J Gen Virol. 1974 Jan;22(1):95–103. doi: 10.1099/0022-1317-22-1-95. [DOI] [PubMed] [Google Scholar]
  18. Mogensen K. E., Pyhälä L., Cantell K. Raising antibodies to human leukocyte interferon. Acta Pathol Microbiol Scand B. 1975 Oct;83(5):443–450. doi: 10.1111/j.1699-0463.1975.tb00123.x. [DOI] [PubMed] [Google Scholar]
  19. Mogensen K. E., Pyhälä L., Törmä E., Cantell K. No evidence for a carbohydrate moiety affecting the clearance of circulating human leukocyte interferon in rabbits. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):305–310. doi: 10.1111/j.1699-0463.1974.tb02331.x. [DOI] [PubMed] [Google Scholar]
  20. Ogburn C. A., Berg K., Paucker K. Purification of mouse interferon by affinity chromatography on anti-interferon globulin-sepharose. J Immunol. 1973 Oct;111(4):1206–1218. [PubMed] [Google Scholar]
  21. PAUCKER K., CANTELL K. Neutralization of interferon by specific antibody. Virology. 1962 Sep;18:145–147. doi: 10.1016/0042-6822(62)90189-7. [DOI] [PubMed] [Google Scholar]
  22. PAUCKER K. THE SEROLOGIC SPECIFICITY OF INTERFERON. J Immunol. 1965 Mar;94:371–378. [PubMed] [Google Scholar]
  23. Paucker K. Antigenic properties of interferon. Tex Rep Biol Med. 1977;35:23–28. [PubMed] [Google Scholar]
  24. Paucker K., Dalton B. J., Ogburn C. A., Törmä E. Multiple active sites on human interferons. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4587–4591. doi: 10.1073/pnas.72.11.4587. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Paucker K., Dalton B. J., Törmä E. T., Ogburn C. A. Biological properties of human leukocyte interferon components. J Gen Virol. 1977 May;35(2):341–351. doi: 10.1099/0022-1317-35-2-341. [DOI] [PubMed] [Google Scholar]
  26. RILEY V. Adaptation of orbital bleeding technic to rapid serial blood studies. Proc Soc Exp Biol Med. 1960 Aug-Sep;104:751–754. doi: 10.3181/00379727-104-25975. [DOI] [PubMed] [Google Scholar]
  27. Sipe J. D., de Maeyer-Guignard J., Fauconnier B., de Maeyer E. Purification of mouse interferon by affinity chromatography on a solid-phase immunoadsorbent. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1037–1040. doi: 10.1073/pnas.70.4.1037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Skurkovich S. V., Eremkina E. I. The probable role of interferon in allergy. Ann Allergy. 1975 Dec;35(6):356–360. [PubMed] [Google Scholar]
  29. Skurkovich S. V., Klinova E. G., Eremkina E. I., Levina N. V. Immunosuppressive effect of an anti-interferon serum. Nature. 1974 Feb 22;247(5442):551–552. doi: 10.1038/247551a0. [DOI] [PubMed] [Google Scholar]
  30. Stewart W. E., 2nd, Desmyter J. Molecular heterogeneity of human leukocyte interferon: two populations differing in molecular weights, requirements for renaturation, and cross-species antiviral activity. Virology. 1975 Sep;67(1):68–73. doi: 10.1016/0042-6822(75)90403-1. [DOI] [PubMed] [Google Scholar]
  31. Strander H., Cantell K., Carlström G., Jakobsson P. A. Clinical and laboratory investigations on man: systemic administration of potent interferon to man. J Natl Cancer Inst. 1973 Sep;51(3):733–742. doi: 10.1093/jnci/51.3.733. [DOI] [PubMed] [Google Scholar]
  32. Strander H., Cantell K. Production of interferon by human leukocytes in vitro. Ann Med Exp Biol Fenn. 1966;44(2):265–273. [PubMed] [Google Scholar]
  33. Strander H., Mogensen K. E., Cantell K. Production of human lymphoblastoid interferon. J Clin Microbiol. 1975 Jan;1(1):116–117. doi: 10.1128/jcm.1.1.116-117.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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