Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1978 Feb;19(2):711–719. doi: 10.1128/iai.19.2.711-719.1978

Cell-associated levan of Actinomyces viscosus.

T N Warner, C H Miller
PMCID: PMC414138  PMID: 344220

Abstract

Actinomyces viscosus ATCC 15987 was examined for the presence of cell-associated levan by absorption of myeloma proteins with antilevan activity and direct immunofluorescence. Levan was not detectable on the surface of glucose-grown A. viscosus, but after a brief incubation of these cells with 5% sucrose, they were encapsulated with tenaciously adhering levan. The levan layer constituted between 0.02 and 0.03% of the cell dry weight. In contrast, sucrose-grown A. viscosus cells possessed a low level of cell-associated levan, which was only moderately increased by incubation in sucrose and which partially existed as a loose slime rather than a tenacious capsule.

Full text

PDF
711

Images in this article

714Image
on p.714
718Image
on p.718

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker J. J., Chan S. P., Socransky S. S., Oppenheim J. J., Mergenhagen S. E. Importance of Actinomyces and certain gram-negative anaerobic organisms in the transformation of lymphocytes from patients with periodontal disease. Infect Immun. 1976 May;13(5):1363–1368. doi: 10.1128/iai.13.5.1363-1368.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cisar J., Kabat E. A., Liao J., Potter M. Immunochemical studies on mouse myeloma proteins reactive with dextrans or with fructosans and on human antilevans. J Exp Med. 1974 Jan 1;139(1):159–179. doi: 10.1084/jem.139.1.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ellen R. P. Establishment and distribution of Actinomyces viscosus and Actinomyces naeslundii in the human oral cavity. Infect Immun. 1976 Nov;14(5):1119–1124. doi: 10.1128/iai.14.5.1119-1124.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Engel D., Epps D. V., Clagett J. In vivo and in vitro studies on possible pathogenic mechanisms of Actinomyces viscosus. Infect Immun. 1976 Aug;14(2):548–554. doi: 10.1128/iai.14.2.548-554.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FULTHORPE A. J. Multiple diphtheria antigen-antibody systems investigated by passive haemagglutination techniques and other methods. Immunology. 1962 Jan;5:30–45. [PMC free article] [PubMed] [Google Scholar]
  6. Guggenheim B., Schroeder H. E. Reactions in the periodontium to continuous antigenic stimulation in sensitized gnotobiotic rats. Infect Immun. 1974 Sep;10(3):565–577. doi: 10.1128/iai.10.3.565-577.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Howell A., Jr, Jordan H. V. Production of an extracellular levan by Odontomyces viscosus. Arch Oral Biol. 1967 Apr;12(4):571–573. doi: 10.1016/0003-9969(67)90033-7. [DOI] [PubMed] [Google Scholar]
  8. Hämmerling U., Westphal O. Synthesis and use of O-stearoyl polysaccharides in passive hemagglutination and hemolysis. Eur J Biochem. 1967 Mar;1(1):46–50. doi: 10.1007/978-3-662-25813-2_9. [DOI] [PubMed] [Google Scholar]
  9. Irving J. T., Socransky S. S., Heeley J. D. Histological changes in experimental periodontal disease in gnotobiotic rats and conventional hamsters. J Periodontal Res. 1974;9(2):73–80. doi: 10.1111/j.1600-0765.1974.tb00656.x. [DOI] [PubMed] [Google Scholar]
  10. JORDAN H. V., KEYES P. H. AEROBIC, GRAM-POSITIVE, FILAMENTOUS BACTERIA AS ETIOLOGIC AGENTS OF EXPERIMENTAL PERIODONTAL DISEASE IN HAMSTERS. Arch Oral Biol. 1964 Jul-Aug;9:401–414. doi: 10.1016/0003-9969(64)90025-1. [DOI] [PubMed] [Google Scholar]
  11. Jordan H. V., Keyes P. H., Bellack S. Periodontal lesions in hamsters and gnotobiotic rats infected with actinomyces of human origin. J Periodontal Res. 1972;7(1):21–28. doi: 10.1111/j.1600-0765.1972.tb00627.x. [DOI] [PubMed] [Google Scholar]
  12. KEYES P. H., JORDAN H. V. PERIODONTAL LESIONS IN THE SYRIAN HAMSTER. III. FINDINGS RELATED TO AN INFECTIOUS AND TRANSMISSIBLE COMPONENT. Arch Oral Biol. 1964 Jul-Aug;9:377–400. doi: 10.1016/0003-9969(64)90024-x. [DOI] [PubMed] [Google Scholar]
  13. Krichevsky M. I., Howell A., Jr, Lim S. Levan formation by Odontomyces viscosus. J Dent Res. 1969 Sep-Oct;48(5):938–942. doi: 10.1177/00220345690480055701. [DOI] [PubMed] [Google Scholar]
  14. Lehner T., Wilton J. M., Challacombe S. J., Ivanyi L. Sequential cell-mediated immune responses in experimental gingivitis in man. Clin Exp Immunol. 1974 Mar;16(3):481–492. [PMC free article] [PubMed] [Google Scholar]
  15. Llory H., Guillo B., Frank R. M. A cariogenic Actinomyces viscosus--a bacteriological and gnotobiotic study. Helv Odontol Acta. 1971 Oct;15(2):134–138. [PubMed] [Google Scholar]
  16. Miller C. H., Warner T. N., Palenik C. J., Somers P. J. Levan formation by whole cells of Actinomyces viscosus ATCC 15987. J Dent Res. 1975 Jul-Aug;54(4):906–906. doi: 10.1177/00220345750540043701. [DOI] [PubMed] [Google Scholar]
  17. Pabst M. J. Levan and levansucrase of Actinomyces viscosus. Infect Immun. 1977 Feb;15(2):518–526. doi: 10.1128/iai.15.2.518-526.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Patters M. R., Genco R. J., Reed M. J., Mashimo P. A. Blastogenic response of human lymphocytes to oral bacterial antigens: comparison of individuals with periodontal disease to normal and edentulous subjects. Infect Immun. 1976 Nov;14(5):1213–1220. doi: 10.1128/iai.14.5.1213-1220.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. WILKINSON J. F. The extracellualr polysaccharides of bacteria. Bacteriol Rev. 1958 Mar;22(1):46–73. doi: 10.1128/br.22.1.46-73.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Williams B. L., Pantalone R. M., Sherris J. C. Subgingival microflora and periodontitis. J Periodontal Res. 1976 Feb;11(1):1–18. doi: 10.1111/j.1600-0765.1976.tb00045.x. [DOI] [PubMed] [Google Scholar]
  21. van Houte J., Jansen H. M. Levan degradation by streptococci isolated from human dental plaque. Arch Oral Biol. 1968 Jul;13(7):827–830. doi: 10.1016/0003-9969(68)90102-7. [DOI] [PubMed] [Google Scholar]
  22. van der Hoeven J. S., Mikx F. H., König K. G., Plasschaert A. J. Plaque formation and dental caries in gnotobiotic and SPF Osborne-Mendel rats associated with Actinomyces viscosus. Caries Res. 1974;8(3):211–223. doi: 10.1159/000260110. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES