Abstract
The ratios of ribonucleic acid to protein contents of Clostridium botulinum type C, D, and E cultures were lower than those of type A, B, and F cultures. Addition of ribonucleic acid at 0.4 mg/ml to culture satisfactorily aided acid precipitation of type C and D toxins, but not that of type E toxin.
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Selected References
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- Boroff D. A., Fleck U. Statistical analysis of a rapid in vivo method for the titration of the toxin of Clostridium botulinum. J Bacteriol. 1966 Nov;92(5):1580–1581. doi: 10.1128/jb.92.5.1580-1581.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CARDELLA M. A., DUFF J. T., GOTTFRIED C., BEGEL J. S. Studies on immunity to toxins of Clostridium botulinum. IV. Production and purification of type C toxin for conversion to toxoid. J Bacteriol. 1958 Mar;75(3):360–365. doi: 10.1128/jb.75.3.360-365.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CARDELLA M. A., DUFF J. T., WINGFIELD B. H., GOTTFRIED C. Studies on immunity to toxins of Clostridium botulinum. VI. Purification and detoxification of type D toxin and the immunological response to toxoid. J Bacteriol. 1960 Mar;79:372–378. doi: 10.1128/jb.79.3.372-378.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GORDON M., FIOCK M. A., YARINSKY A., DUFF J. T. Studies on immunity to toxins of Clostridium botulinum. III. Preparation, purification, and detoxification of type E toxin. J Bacteriol. 1957 Oct;74(4):533–538. doi: 10.1128/jb.74.4.533-538.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kitamura M., Sakaguchi S., Sakaguchi G. Purification and some properties of Clostridium botulinum type-E toxin. Biochim Biophys Acta. 1968 Oct 21;168(2):207–217. doi: 10.1016/0005-2795(68)90144-x. [DOI] [PubMed] [Google Scholar]
- Kozaki S., Sakaguchi S., Sakaguchi G. Purification and some properties of progenitor toxins of Clostridium botulinum type B. Infect Immun. 1974 Oct;10(4):750–756. doi: 10.1128/iai.10.4.750-756.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lamanna C., McElroy O. E., Eklund H. W. The Purification and Crystallization of Clostridium botulinum Type A Toxin. Science. 1946 May 17;103(2681):613–614. doi: 10.1126/science.103.2681.613. [DOI] [PubMed] [Google Scholar]
- Miyazaki S., Iwasaki M., Sakaguchi G. Clostridium botulinum type D toxin: purification, molecular structure, and some immunological properties. Infect Immun. 1977 Aug;17(2):395–401. doi: 10.1128/iai.17.2.395-401.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oishi I., Sakaguchi G. Purification of Clostridium botuliunum type F progenitor toxin. Appl Microbiol. 1974 Dec;28(6):923–928. doi: 10.1128/am.28.6.923-928.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sugii S., Sakaguchi G. Molecular construction of Clostridium botulinum type A toxins. Infect Immun. 1975 Dec;12(6):1262–1270. doi: 10.1128/iai.12.6.1262-1270.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]