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. 1979 Feb;23(2):347–352. doi: 10.1128/iai.23.2.347-352.1979

Cell-mediated immune response to bacterial products in human tonsils and peripheral blood lymphocytes.

M M Drucker, Y Agatsuma, I Drucker, E Neter, J Bernstein, P L Ogra
PMCID: PMC414171  PMID: 311345

Abstract

Lymphoproliferative responses of tonsillar tissue lymphocytes and peripheral blood lymphocytes to phytohemagglutinin and specific bacterial product antigens were studied in children undergoing tonsillectomy and adenoidectomy. Tonsillar tissue lymphocytes responded to optimal concentrations of phytohemagglutinin. Varidase, and streptolysin-O in a manner similar to peripheral blood lymphocytes. Higher base-line mitogenic activity in tonsillar lymphocytes was frequently associated with the presence of Staphylococcus aureus in the tonsils. Tonsillar tissue lymphocytes from 23% of the subjects with the highest base-line mitogenic activity manifested a decreased response to in vitro stimulation with mitogens or antigens. In subjects with such preactivated tonsillar lymphocytes, the proliferative responsiveness of blood lymphocytes to mitogen and antigens was markedly increased after tonsillectomy and adenoidectomy. These observations suggest the existence of in vitro correlates of cellular immunity to bacterial products in the mucosal surfaces. In addition, it is proposed that tonsils may possess immunosuppressive activity for peripheral blood lymphocytes, which may be related to local tonsillar infections.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fauci A. S., Pratt K. R., Whalen G. Activation of human B lymphocytes. II. Cellular interactions in the PFC response of human tonsillar and peripheral blood B lymphocytes to polyclonal activation by pokeweed mitogen. J Immunol. 1976 Dec;117(6):2100–2104. [PubMed] [Google Scholar]
  2. Forsgren A., Svedjelund A., Wigzell H. Lymphocyte stimulation by protein A of Staphylococcus aureus. Eur J Immunol. 1976 Mar;6(3):207–213. doi: 10.1002/eji.1830060312. [DOI] [PubMed] [Google Scholar]
  3. Henle G., Henle W. Immunofluorescence in cells derived from Burkitt's lymphoma. J Bacteriol. 1966 Mar;91(3):1248–1256. doi: 10.1128/jb.91.3.1248-1256.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hubert C., Delespesse G., Govaerts A. Concanavalin A-activated suppressor cells in normal human peripheral blood lymphocytes. Clin Exp Immunol. 1976 Oct;26(1):95–98. [PMC free article] [PubMed] [Google Scholar]
  5. Kauffman C. A., Linnemann C. C., Jr, Schiff G. M., Phair J. P. Effect of viral and bacterial pneumonias on cell-mediated immunity in humans. Infect Immun. 1976 Jan;13(1):78–83. doi: 10.1128/iai.13.1.78-83.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mogensen C. E. The glomerular permeability determined by dextran clearance using Sephadex gel filtration. Scand J Clin Lab Invest. 1968;21(1):77–82. doi: 10.3109/00365516809076979. [DOI] [PubMed] [Google Scholar]
  7. Morag A., Morag B., Bernstein J. M., Beutner K., Ogra P. L. In vitro correlates of cell-mediated immunity in human tonsils after natural or induced Rubella virus infection. J Infect Dis. 1975 Apr;131(4):409–416. doi: 10.1093/infdis/131.4.409. [DOI] [PubMed] [Google Scholar]
  8. Mudd S., Shayegani M. Delayed-type hypersensitivity to S. aureus and its uses. Ann N Y Acad Sci. 1974 Jul 31;236(0):244–251. doi: 10.1111/j.1749-6632.1974.tb41495.x. [DOI] [PubMed] [Google Scholar]
  9. Oettgen H. F., Silber R., Miescher P. A., Hirschhorn K. Stimulation of human tonsillar lymphocytes in vitro. Clin Exp Immunol. 1966 Jan;1(1):77–84. [PMC free article] [PubMed] [Google Scholar]
  10. Patters M. R., Genco R. J., Reed M. J., Mashimo P. A. Blastogenic response of human lymphocytes to oral bacterial antigens: comparison of individuals with periodontal disease to normal and edentulous subjects. Infect Immun. 1976 Nov;14(5):1213–1220. doi: 10.1128/iai.14.5.1213-1220.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pross H. F., Eidinger D. Antigenic competition: a review of nonspecific antigen-induced suppression. Adv Immunol. 1974;18:133–168. doi: 10.1016/s0065-2776(08)60309-0. [DOI] [PubMed] [Google Scholar]
  12. Reed M. J., Patters M. R., Mashimo P. A., Genco R. J., Levine R. J. Blastogenic response of human lymphocytes to oral bacterial antigens: characterization of bacterial sonicates. Infect Immun. 1976 Nov;14(5):1202–1212. doi: 10.1128/iai.14.5.1202-1212.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Reedman B. M., Klein G. Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines. Int J Cancer. 1973 May;11(3):499–520. doi: 10.1002/ijc.2910110302. [DOI] [PubMed] [Google Scholar]
  14. Rich R. R., Rich S. S. Biological expressions of lymphocyte activation. IV. Concanavalin A-activated suppressor cells in mouse mixed lymphocyte reactions. J Immunol. 1975 Mar;114(3):1112–1115. [PubMed] [Google Scholar]
  15. Schwab J. H. Suppression of the immune response by microorganisms. Bacteriol Rev. 1975 Jun;39(2):121–143. doi: 10.1128/br.39.2.121-143.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Taranta A. Lymphocyte mitogens of staphylococcal origin. Ann N Y Acad Sci. 1974 Jul 31;236(0):362–375. doi: 10.1111/j.1749-6632.1974.tb41503.x. [DOI] [PubMed] [Google Scholar]
  17. Wagnerová M., Stafpvá J., Wagner V., Vacek V. Transformation of lymphocytes in patients with certain bacterial infections. Med Microbiol Immunol. 1975 Sep 19;161(4):273–277. doi: 10.1007/BF02122715. [DOI] [PubMed] [Google Scholar]
  18. Yamamoto N., Katsuki T., Hinuma Y. Transformation of tonsil lymphocytes by Epstein-Barr virus. J Natl Cancer Inst. 1976 Jun;56(6):1105–1107. doi: 10.1093/jnci/56.6.1105. [DOI] [PubMed] [Google Scholar]
  19. Yoshinaga M., Nakamura S., Hayashi H. Interaction between lymphocytes and inflammatory exudate cells. I. Enhacement of thymocyte response to PHA by product(s) of polymorphonuclear leukocytes and macrophages. J Immunol. 1975 Aug;115(2):533–538. [PubMed] [Google Scholar]

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