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. 2014 Aug 13;3:430. doi: 10.1186/2193-1801-3-430

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© Piwpankaew et al.; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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SDS-PAGE and zymogram analysis of recombinant β-mannanase from E. coli expression system. (A) SDS-PAGE : lane M, protein marker (Invitrogen, USA); lane 1, crude culture supernatant; lane 2, crude cell lysate. (B) zymogram analysis: lane 1, crude culture supernatant; lane 2, crude cell lysate.