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. 1979 Mar;23(3):587–591. doi: 10.1128/iai.23.3.587-591.1979

Bactericidal activity of fractionated granule contents from human polymorphonuclear leukocytes.

M C Modrzakowski, M H Cooney, L E Martin, J K Spitznagel
PMCID: PMC414206  PMID: 378830

Abstract

Proteins from human polymorphonuclear leukocyte granules were extracted with 0.2 M acetate, pH 4.0, and fractionated by Sephadex G-100 column chromatography. The fractions demonstrated selective bactericidal action against a deep rough cell wall mutant of Escherichia coli O111:B4 with rough lipopolysacharide and cell wall mutants of Salmonella typhimurium LT-2 with lipoplysacharide of Ra, Rc, Rd1, Rd2, and Re types. Smooth parent strains were most resistant to the bactericidal action. Fractions with greatest activity for the mutants were from valley regions (regions of low protein concentration) between three high protein peaks comprising myeloperoxidase, protease, and lysozyme, respectively. Susceptibility of the mutants to bactericidal action increased as sugar residues decreased in lipopolysaccharide. Gram-positive bacteria were susceptible to different fractions than were the gram-negative bacteria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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