Abstract
The influence of culture media on various properties of Streptococcus mutans was investigated. Strains of S. mutans (serotypes c, d, f, and g) were grown in a complex medium (Todd-Hewitt broth [THB]) or a synthetic medium (SYN). The SYN cells, in contrast to THB cells, did not bind extracellular glucosyltransferase and did not produce in vitro adherence. Both types of cells possessed constitutive levels of glucosyltransferase. B13 cells grown in SYN plus invertase-treated glucose possessed the same level of constitutive enzyme as THB cells. In contrast to THB cells, the SYN cells of seven serotype strains did not agglutinate upon the addition of high-molecular-weight dextran/glucan. Significant quantities of lower-molecular-weight (2 × 104 or 7 × 104) dextran and B13 glucan were bound by SYN cells. SYN cells agglutinated weakly in anti-glucan serum (titers, 0 to 16), whereas THB cells possessed titers of 32 to 256. Evidence for the existence of a second binding site in agglutination which does not possess a glucan-like polymer has been obtained. B13 cells grown in invertase-treated THB agglutinated to the same degree as normal THB cells. The nature of this site is unknown. SYN cells possess the type-specific polysaccharide antigen. B13 cells did not bind from THB a glycoprotein which reacts with antisera to the A, B, or T blood group antigens or which allows agglutination upon the addition of dextran. The results demonstrate that S. mutans grown in a chemically defined medium possesse markedly different biochemical and biological activities than cells grown in a complex organic medium.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAILEY R. W. Transglucosidase activity of rumen strains of Streptococcus bovis. 2. Isolation and properties of dextransucrase. Biochem J. 1959 May;72(1):42–49. doi: 10.1042/bj0720042. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carlsson J. Chemically defined medium for growth of Streptococcus sanquis. Caries Res. 1970;4(4):297–304. doi: 10.1159/000259652. [DOI] [PubMed] [Google Scholar]
- Carlsson J., Elander B. Regulation of dextransucrase formation by Streptococcus sanguis. Caries Res. 1973;7(2):89–101. doi: 10.1159/000259834. [DOI] [PubMed] [Google Scholar]
- Carlsson J. Nutritional requirements of Streptococcus mutans. Caries Res. 1970;4(4):305–320. doi: 10.1159/000259653. [DOI] [PubMed] [Google Scholar]
- Germaine G. R., Schachtele C. F. Streptococcus mutans dextransucrase: mode of interaction with high-molecular-weight dextran and role in cellular aggregation. Infect Immun. 1976 Feb;13(2):365–372. doi: 10.1128/iai.13.2.365-372.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gibbons R. J., Fitzgerald R. J. Dextran-induced agglutination of Streptococcus mutans, and its potential role in the formation of microbial dental plaques. J Bacteriol. 1969 May;98(2):341–346. doi: 10.1128/jb.98.2.341-346.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gibbons R. J., van Houte J. On the formation of dental plaques. J Periodontol. 1973 Jun;44(6):347–360. doi: 10.1902/jop.1973.44.6.347. [DOI] [PubMed] [Google Scholar]
- Guggenheim B., Schroeder H. E. Biochemical and morphological aspects of extracellular polysaccharides produced by cariogenic streptococci. Helv Odontol Acta. 1967 Oct;11(2):131–152. [PubMed] [Google Scholar]
- Guggenheim B. Streptococci of dental plaques. Caries Res. 1968;2(2):147–163. doi: 10.1159/000259553. [DOI] [PubMed] [Google Scholar]
- HESS E. L., SLADE H. D. An electrophoretic examination of cell-free extracts from various serological types of group A hemolytic streptococci. Biochim Biophys Acta. 1955 Mar;16(3):346–353. doi: 10.1016/0006-3002(55)90237-7. [DOI] [PubMed] [Google Scholar]
- Hamada S., Kobayashi Y., Slade H. D. Cell-bound synthesis and subsequent adherence of oral streptococci due to the binding of extracellular glucosyltransferase to the streptococcal cell surface. Microbiol Immunol. 1978;22(5):279–282. doi: 10.1111/j.1348-0421.1978.tb00373.x. [DOI] [PubMed] [Google Scholar]
- Hamada S., Slade H. D. Adherence of serotype e Streptococcus mutans and the inhibitory effect of Lancefield group E and S mutans type e antiserum. J Dent Res. 1976 Apr;55(Spec No):C65–C74. doi: 10.1177/002203457605500328011. [DOI] [PubMed] [Google Scholar]
- Hamada S., Tai S., Slade H. D. Binding of glucosyltransferase and glucan synthesis by Streptococcus mutans and other bacteria. Infect Immun. 1978 Jul;21(1):213–220. doi: 10.1128/iai.21.1.213-220.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hamada S., Tai S., Slade H. D. Selective adsorption of heterophile polyglycerophosphate antigen from antigen extracts of Streptococcus mutans and other gram-positive bacteria. Infect Immun. 1976 Oct;14(4):903–910. doi: 10.1128/iai.14.4.903-910.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hamada S., Torii M. Effect of sucrose in culture media on the location of glucosyltransferase of Streptococcus mutans and cell adherence to glass surfaces. Infect Immun. 1978 Jun;20(3):592–599. doi: 10.1128/iai.20.3.592-599.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Higgins M. L., Shockman G. D. Early changes in the ultrastructure of Streptococcus faecalis after amino acid starvation. J Bacteriol. 1970 Jul;103(1):244–253. doi: 10.1128/jb.103.1.244-253.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Janda W. M., Kuramitsu H. K. Properties of a variant of Streptococcus mutans altered in its ability to interact with glucans. Infect Immun. 1977 May;16(2):575–586. doi: 10.1128/iai.16.2.575-586.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Janda W. M., Kuramitsu H. K. Regulation and extracellular glucosyltransferase production and the relationship between extracellular and cell-associated activities in Streptococcus mutans. Infect Immun. 1976 Jul;14(1):191–202. doi: 10.1128/iai.14.1.191-202.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kelstrup J., Funder-Nielsen T. D. Adhesion of dextran to Streptococcus mutans. J Gen Microbiol. 1974 Apr;81(2):485–489. doi: 10.1099/00221287-81-2-485. [DOI] [PubMed] [Google Scholar]
- Kuramitsu H. K. Characterization of cell-associated dextransucrase activity from glucose-grown cells of Streptococcus mutans. Infect Immun. 1974 Jul;10(1):227–235. doi: 10.1128/iai.10.1.227-235.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuramitsu H. K. Characterization of invertase activity from cariogenic Streptococcus mutans. J Bacteriol. 1973 Sep;115(3):1003–1010. doi: 10.1128/jb.115.3.1003-1010.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kuramitsu H. K., Ingersoll L. Interaction of glucosyltransferase with the cell surface of Streptococcus mutans. Infect Immun. 1978 Jun;20(3):652–659. doi: 10.1128/iai.20.3.652-659.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lawson J. W. Growth of cariogenic streptococci in chemically defined medium. Arch Oral Biol. 1971 Mar;16(3):339–342. doi: 10.1016/0003-9969(71)90025-2. [DOI] [PubMed] [Google Scholar]
- Linzer R., Slade H. D. Characterization of an anti-glucosyltransferase serum specific for insoluble glucan synthesis by Streptococcus mutans. Infect Immun. 1976 Feb;13(2):494–500. doi: 10.1128/iai.13.2.494-500.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Linzer R., Slade H. D. Purification and characterization of Streptococcus mutans group d cell wall polysaccharide antigen. Infect Immun. 1974 Aug;10(2):361–368. doi: 10.1128/iai.10.2.361-368.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Luzzio A. J. Demonstration of blood group substance A bound to Pasteurella pestis. Proc Soc Exp Biol Med. 1969 Jul;131(3):853–858. doi: 10.3181/00379727-131-33993. [DOI] [PubMed] [Google Scholar]
- McBride B. C., Bourgeau G. Dextran-induced aggregation of Actinomyces viscosus. Arch Oral Biol. 1975 Dec;20(12):837–841. doi: 10.1016/0003-9969(75)90063-1. [DOI] [PubMed] [Google Scholar]
- McCabe M. M., Smith E. E. Origin of the cell-associated dextransucrase of Streptococcus mutans. Infect Immun. 1973 Jun;7(6):829–838. doi: 10.1128/iai.7.6.829-838.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Montville T. J., Cooney C. L., Sinskey A. J. Distribution of dextransucrase in Streptococcus mutans and observations on the effect of soluble dextran on dextransucrase activities. Infect Immun. 1977 Dec;18(3):629–635. doi: 10.1128/iai.18.3.629-635.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mukasa H., Slade H. D. Mechanism of adherence of Streptococcus mutans to smooth surfaces. I. Roles of insoluble dextran-levan synthetase enzymes and cell wall polysaccharide antigen in plaque formation. Infect Immun. 1973 Oct;8(4):555–562. doi: 10.1128/iai.8.4.555-562.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mukasa H., Slade H. D. Mechanism of adherence of Streptococcus mutans to smooth surfaces. II. Nature of the binding site and the adsorption of dextran-levan synthetase enzymes on the cell-wall surface of the streptococcus. Infect Immun. 1974 Feb;9(2):419–429. doi: 10.1128/iai.9.2.419-429.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nord C. E., Linder L., Wadström T., Lindberg A. A. Formation of glycoside-hydrolases by oral streptococci. Arch Oral Biol. 1973 Mar;18(3):391–402. doi: 10.1016/0003-9969(73)90163-5. [DOI] [PubMed] [Google Scholar]
- Olson G. A., Guggenheim B., Small P. A., Jr Antibody-mediated inhibition of dextran-sucrose-induced agglutination of Streptococcus mutans. Infect Immun. 1974 Feb;9(2):273–278. doi: 10.1128/iai.9.2.273-278.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SHOCKMAN G. D. AMINO-ACID DEPRIVATION AND BACTERIAL CELL-WALL SYNTHESIS. Trans N Y Acad Sci. 1963 Dec;26:182–195. doi: 10.1111/j.2164-0947.1963.tb01241.x. [DOI] [PubMed] [Google Scholar]
- Schachtele C. F., Harlander S. K., Germaine G. R. Streptococcus mutans dextransucrase: availability of disaggregated enzyme after growth in a chemically defined medium. Infect Immun. 1976 May;13(5):1522–1524. doi: 10.1128/iai.13.5.1522-1524.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scherp H. W. Dental caries: prospects for prevention. Science. 1971 Sep 24;173(4003):1199–1205. doi: 10.1126/science.173.4003.1199. [DOI] [PubMed] [Google Scholar]
- Springer G. F. Blood-group and Forssman antigenic determinants shared between microbes and mammalian cells. Prog Allergy. 1971;15:9–77. [PubMed] [Google Scholar]
- Springer G. F., Desai P. R., Scanlon E. F. Blood group MN precursors as human breast carcinoma-associated antigens and "naturally" occurring human cytotoxins against them. Cancer. 1976 Jan;37(1):169–176. doi: 10.1002/1097-0142(197601)37:1<169::aid-cncr2820370124>3.0.co;2-#. [DOI] [PubMed] [Google Scholar]
- Tanzer J. M., Brown A. T., McInerney M. F., Woodiel F. N. Comparative study of invertases of Streptococcus mutans. Infect Immun. 1977 Apr;16(1):318–327. doi: 10.1128/iai.16.1.318-327.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tanzer J. M., Wood W. I., Krichevsky M. I. Linear growth kinetics of plaque-forming streptococci in the presence of sucrose. J Gen Microbiol. 1969 Sep;58(1):125–133. doi: 10.1099/00221287-58-1-125. [DOI] [PubMed] [Google Scholar]
- Terleckyj B., Shockman G. D. Amino acid requirements of Streptococcus mutans and other oral streptococci. Infect Immun. 1975 Apr;11(4):656–664. doi: 10.1128/iai.11.4.656-664.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Terleckyj B., Willett N. P., Shockman G. D. Growth of several cariogenic strains of oral streptococci in a chemically defined medium. Infect Immun. 1975 Apr;11(4):649–655. doi: 10.1128/iai.11.4.649-655.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wittenberger C. L., Beaman A. J., Lee L. N. Tween 80 effect on glucosyltransferase synthesis by Streptococcus salivarius. J Bacteriol. 1978 Jan;133(1):231–239. doi: 10.1128/jb.133.1.231-239.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Houte J., Winkler K. C., Jansen H. M. Iodophilic polysaccharide synthesis, acid production and growth in oral streptococci. Arch Oral Biol. 1969 Jan;14(1):45–61. doi: 10.1016/0003-9969(69)90020-x. [DOI] [PubMed] [Google Scholar]