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. 1979 Mar;23(3):743–750. doi: 10.1128/iai.23.3.743-750.1979

Local cholera immunity in mice: intestinal antitoxin-containing cells and their correlation with protective immunity.

S Lange, H A Hansson, S O Molin, H Nygren
PMCID: PMC414229  PMID: 457257

Abstract

Methods for light and electron microscopic identification and characterization of intestinal cholera antitoxin-containing cells (ACC) using peroxidase-labeled immunoreagent are described and used to study the ACC response in mice after immunizations with cholera toxin. Specific ACC appeared in significant numbers after two oral immunizations and increased six- to eightfold with two additional oral boosters, whereas further oral immunizations caused no additional stimulation. Intravenous immunizations had to be repeated seven times before ACC could be detected. After two oral immunizations, most of the ACC were found in the proximal part of the small intestine and no ACC were seen in the colon. This uneven distribution of ACC within the small intestine was eliminated after four oral immunizations, when ACC could also be detected in the colon. The ACC response after four oral immunizations demonstrated a peak at 4 days with far fewer cells present at 2 and 7 days. Electron microscopic studies showed that the ACC were mature plasma cells with the staining localized to the endoplasmic reticulum. Regression analysis of the relationship between the number of ACC and the magnitude of protective immunity against intestinal challenge with cholera toxin indicates a highly significant correlation (r = 0.91, P less than 0.001).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrameas S., Ternynck T. Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry. 1971 Dec;8(12):1175–1179. doi: 10.1016/0019-2791(71)90395-8. [DOI] [PubMed] [Google Scholar]
  2. Boorsma D. M., Kalsbeek G. L. A comparative study of horseradish peroxidase conjugates prepared with a one-step and a two-step method. J Histochem Cytochem. 1975 Mar;23(3):200–207. doi: 10.1177/23.3.47869. [DOI] [PubMed] [Google Scholar]
  3. Craig S. W., Cebra J. J. Peyer's patches: an enriched source of precursors for IgA-producing immunocytes in the rabbit. J Exp Med. 1971 Jul 1;134(1):188–200. doi: 10.1084/jem.134.1.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fubara E. S., Freter R. Protection against enteric bacterial infection by secretory IgA antibodies. J Immunol. 1973 Aug;111(2):395–403. [PubMed] [Google Scholar]
  5. GOWANS J. L., KNIGHT E. J. THE ROUTE OF RE-CIRCULATION OF LYMPHOCYTES IN THE RAT. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:257–282. doi: 10.1098/rspb.1964.0001. [DOI] [PubMed] [Google Scholar]
  6. Hansson H. A., Holmgren J., Svennerholm L. Ultrastructural localization of cell membrane GM1 ganglioside by cholera toxin. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3782–3786. doi: 10.1073/pnas.74.9.3782. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Holmgren J., Lindholm L., Lönnroth I. Interaction of cholera toxin and toxin derivatives with lymphocytes. I. Binding properties and interference with lectin-induced cellular stimulation. J Exp Med. 1974 Apr 1;139(4):801–819. doi: 10.1084/jem.139.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Holmgren J., Lonnroth I. Oligomeric structure of cholera toxin: characteristics of the H and L subunits. J Gen Microbiol. 1975 Jan;86(1):49–65. doi: 10.1099/00221287-86-1-49. [DOI] [PubMed] [Google Scholar]
  9. Lange S., Holmgren J. Protective antitoxic cholera immunity in mice: influence of route and number of immunizations and mode of action of protective antibodies. Acta Pathol Microbiol Scand C. 1978 Aug;86C(4):145–152. doi: 10.1111/j.1699-0463.1978.tb02572.x. [DOI] [PubMed] [Google Scholar]
  10. Molin S. O., Nygren H., Dolonius L. A new method for the study of glutaraldehyde-induced crosslinking properties in proteins with special reference to the reaction with amino groups. J Histochem Cytochem. 1978 May;26(5):412–414. doi: 10.1177/26.5.96177. [DOI] [PubMed] [Google Scholar]
  11. Molin S. O., Nygren H., Hansson H. A. Binding of glutaraldehyde reacted peroxidase to cell surfaces--a source of error in immunocytochemistry. J Histochem Cytochem. 1978 Apr;26(4):325–326. doi: 10.1177/26.4.96176. [DOI] [PubMed] [Google Scholar]
  12. Ogra P. L., Karzon D. T. The role of immunoglulins in the mechanism of mucosal immunity to virus infection. Pediatr Clin North Am. 1970 May;17(2):385–400. doi: 10.1016/s0031-3955(16)32417-8. [DOI] [PubMed] [Google Scholar]
  13. Pierce N. F., Cray W. C., Jr, Sircar B. K. Induction of a mucosal antitoxin response and its role in immunity to experimental canine cholera. Infect Immun. 1978 Jul;21(1):185–193. doi: 10.1128/iai.21.1.185-193.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pierce N. F., Gowans J. L. Cellular kinetics of the intestinal immune response to cholera toxoid in rats. J Exp Med. 1975 Dec 1;142(6):1550–1563. doi: 10.1084/jem.142.6.1550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Plattner H., Wachter E., Gröbner P. A heme-nonapeptide tracer for electron microscopy. Preparation, characterization and comparison with other heme-tracers. Histochemistry. 1977 Aug 22;53(3):223–242. doi: 10.1007/BF00511078. [DOI] [PubMed] [Google Scholar]
  16. Svennerholm A., Lange S., Holmgren J. Correlation between intestinal synthesis of specific immunoglobulin A and protection against experimental cholera in mice. Infect Immun. 1978 Jul;21(1):1–6. doi: 10.1128/iai.21.1.1-6.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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