Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1979 Apr;24(1):39–46. doi: 10.1128/iai.24.1.39-46.1979

Immunogenicity of Bacteroides isolated from mice: relationship between immunogenicity and cell wall antigens.

H Yasui, N Yasutake, M Ohwaki
PMCID: PMC414258  PMID: 378846

Abstract

Three different strains of Bacteroides were isolated from feces and cecal contents of mice. The immunogenicity of the strains was determined by measuring the serum agglutinin titers after intraperitoneal antigen injection. There were marked differences in quantity and quality of produced antibodies among the three strains. One strain (2-2) induced low antibody titers in both the primary and secondary responses, and a significant 2-mercaptoethanol (2-ME)-resistant antibody production occurred. Another strain (Y) induced low antibody titers in the primary response and high titers in the secondary response, but 2-ME-resistant antibody production did not occur. The third strain (2-4) induced very high antibody titers in both the responses, and a large amount of 2-ME-resistant antibody production occurred. Further, heat-ethanol-treated strain Y induced only immunoglobulin M antibody, but periodate-treated strain Y induced no antibody. Heat-ethanol- or periodate-treated strain 2-4 induced immunoglobulin M or G antibody, respectively. These observations suggest that the surface antigens of the two strains are distinctly different: the antigen of strain Y would be mostly O-antigen, whereas those of strain 2-4 would be O-antigen and protein moieties.

Full text

PDF
39

Images in this article

43Image
on p.43
44Image
on p.44

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAUER D. C., MATHIES M. J., STAVITSKY A. B. Sequences of synthesis of gamma-1 macroglobulin and gamma-2 globulin antibodies during primary and secondary responses to proteins, salmonella antigens, and phage. J Exp Med. 1963 Jun 1;117:889–907. doi: 10.1084/jem.117.6.889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bauer D. C., Stavitsky A. B. ON THE DIFFERENT MOLECULAR FORMS OF ANTIBODY SYNTHESIZED BY RABBITS DURING THE EARLY RESPONSE TO A SINGLE INJECTION OF PROTEIN AND CELLULAR ANTIGENS. Proc Natl Acad Sci U S A. 1961 Oct;47(10):1667–1680. doi: 10.1073/pnas.47.10.1667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berg R. D., Savage D. C. Immune responses of specific pathogen-free and gnotobiotic mice to antigens of indigenous and nonindigenous microorganisms. Infect Immun. 1975 Feb;11(2):320–329. doi: 10.1128/iai.11.2.320-329.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chandler H. M. Rabbit immunoglobulin responses to the flagella, somatic, and protective antigens of a highly protective strain of Clostridium chauvoei. Infect Immun. 1975 Jul;12(1):143–147. doi: 10.1128/iai.12.1.143-147.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DUBOS R., SCHAEDLER R. W., COSTELLO R., HOET P. INDIGENOUS, NORMAL, AND AUTOCHTHONOUS FLORA OF THE GASTROINTESTINAL TRACT. J Exp Med. 1965 Jul 1;122:67–76. doi: 10.1084/jem.122.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davies A. J., Carter R. L., Leuchars E., Wallis V., Dietrich F. M. The morphology of immune reactions in normal, thymectomized and reconstituted mice. 3. Response to bacterial antigens: salmonellar flagellar antigen and pneumococcal plysaccharide. Immunology. 1970 Dec;19(6):945–957. [PMC free article] [PubMed] [Google Scholar]
  7. Foo M. C., Lee A. Immunological response of mice to members of the autochthonous intestinal microflora. Infect Immun. 1972 Oct;6(4):525–532. doi: 10.1128/iai.6.4.525-532.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fukazawa Y., Shinoda T., Tsuchiya T. Response and specificity of antibodies for Candida albicans. J Bacteriol. 1968 Mar;95(3):754–763. doi: 10.1128/jb.95.3.754-763.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GOLDSTEIN G., SLIZYS I. S., CHASE M. W. Studies on fluorescent antibody staining. I. Non-specific fluorescence with fluorescein-coupled sheep anti-rabbit globulins. J Exp Med. 1961 Jul 1;114:89–110. doi: 10.1084/jem.114.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HUMPHREY J. H., PARROTT D. M., EAST J. STUDIES ON GLOBULIN AND ANTIBODY PRODUCTION IN MICE THYMECTOMIZED AT BIRTH. Immunology. 1964 Jul;7:419–439. [PMC free article] [PubMed] [Google Scholar]
  11. Herrmann B., Nowak W. Nachweis agglutinierender Antikörper bei Bacteroides-Infektionen. Z Gesamte Hyg. 1976;22(10):759–762. [PubMed] [Google Scholar]
  12. Hofstad T. Antibodies reacting with lipopolysaccharides from Bacteroides melaninogenicus, in serum from normal human subjects. J Infect Dis. 1974 Mar;129(3):349–352. doi: 10.1093/infdis/129.3.349. [DOI] [PubMed] [Google Scholar]
  13. Hofstad T. Serological properties of lipopolysaccharide from oral stains of Bacteroides melaninogenicus. J Bacteriol. 1969 Mar;97(3):1078–1082. doi: 10.1128/jb.97.3.1078-1082.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kalpaktsoglou P. K., Hong R., Good R. A. The five classes of immunoglobulins in normal C3H and BALB/c mice. Immunology. 1973 Feb;24(2):303–314. [PMC free article] [PubMed] [Google Scholar]
  15. Kristoeffersen T. Immunochemical studies of oral fusobacteria. 6. Distribution of a group reactive antigen among some bacteria and occurrence of antibodies in human sera to this antigen. Acta Pathol Microbiol Scand. 1969;77(4):717–726. [PubMed] [Google Scholar]
  16. LOSPALLUTO J., MILLER W., Jr, DORWARD B., FINK C. W. The formation of macroglobulin antibodies. I. Studies on adult humans. J Clin Invest. 1962 Jul;41:1415–1421. doi: 10.1172/JCI104596. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lee A., Gordon J., Dubos R. Enumeration of the oxygen sensitive bacteria usually present in the intestine of healthy mice. Nature. 1968 Dec 14;220(5172):1137–1139. doi: 10.1038/2201137a0. [DOI] [PubMed] [Google Scholar]
  18. Morotomi M., Watanabe T., Suegara N., Kawai Y., Mutai M. Distribution of indigenous bacteria in the digestive tract of conventional and gnotobiotic rats. Infect Immun. 1975 May;11(5):962–968. doi: 10.1128/iai.11.5.962-968.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sjöberg O., Möller E. Antigen binding cells in tolerant animals. Nature. 1970 Nov 21;228(5273):780–781. doi: 10.1038/228780a0. [DOI] [PubMed] [Google Scholar]
  20. Tannock G. W. Characteristics of Bacteroides isolates from the cecum of conventional mice. Appl Environ Microbiol. 1977 Apr;33(4):745–750. doi: 10.1128/aem.33.4.745-750.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES