Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1979 Apr;24(1):77–82. doi: 10.1128/iai.24.1.77-82.1979

Immunobiology and species distribution of Mycobacterium tuberculosis antigen 5.

T M Daniel, J J Ellner, L S Todd, D W McCoy, V D Payne, P A Anderson, F T Bhe
PMCID: PMC414264  PMID: 110696

Abstract

The immunobiology and mycobacterial species distribution of immunoabsorbent affinity chromatography-purified Mycobacterium tuberculosis antigen 5 have been studied. In delayed hypersensitivity skin tests, antigen 5 was nearly equipotent with tuberculin-purified protein derivative in sensitized guinea pigs. In vitro, antigen 5 was capable of stimulating the production of migration inhibitory factor by cultured lymphocytes from sensitized guinea pigs and humans. Antigen 5 stimulated thymidine incorporation by cultured guinea pig lymphocytes but did not stimulate thymidine incorporation by cultured human lymphocytes. Although erythrocytes were readily sensitized with antigen 5 for passive hemagglutination, their use did not offer any advantage over previous hemagglutination techniques for the serodiagnosis or evaluation of patients with tuberculosis. By immunoelectrophoresis and immunodiffusion, antigen 5 was readily identified in culture filtrates of 10 strains of M. tuberculosis and M. bovis but not in those of 30 strains of 12 other myobacterial species.

Full text

PDF
77

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOYDEN S. V. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med. 1951 Feb;93(2):107–120. doi: 10.1084/jem.93.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  3. CROWLE A. J. A simplified micro double-diffusion agar precipitin technique. J Lab Clin Med. 1958 Nov;52(5):784–787. [PubMed] [Google Scholar]
  4. Chaparas S. D., Thor D. E., Godfrey H. P., Baer H., Hedrick S. R. Tuberculin-active carbohydrate that induces inhibition of macrophage migration but not lymphocyte transformation. Science. 1970 Nov 6;170(3958):637–639. doi: 10.1126/science.170.3958.637. [DOI] [PubMed] [Google Scholar]
  5. Chaparas S. D., Thor D. E., Hedrick S. R. Comparison of lymphocyte transformation, inhibition of macrophage migration and skin tests using dialyzable and nondialyzable tuberculin fractions from Mycobacterium bovis (BCG). J Immunol. 1971 Jul;107(1):149–153. [PubMed] [Google Scholar]
  6. DANIEL T. M. OBSERVATIONS ON THE ANTIBODY RESPONSE OF RABBITS TO MYCOBACTERIAL ANTIGENS. J Immunol. 1965 Jul;95:100–108. [PubMed] [Google Scholar]
  7. DANIEL T. M., WEYAND J. G., Jr, STAVITSKY A. B. MICROMETHODS FOR THE STUDY OF PROTEINS AND ANTIBODIES. IV. FACTORS INVOLVED IN THE PREPARATION AND USE OF A STABLE PREPARATION OF FORMALINIZED, TANNIC ACID-TREATED, PROTEIN-SENSITIZED ERYTHROCYTES FOR DETECTION OF ANTIGEN AND ANTIBODY. J Immunol. 1963 May;90:741–750. [PubMed] [Google Scholar]
  8. Daniel T. M., Anderson P. A. The isolation by immunoabsorbent affinity chromatography and physicochemical characterization of Mycobacterium tuberculosis antigen 5. Am Rev Respir Dis. 1978 Mar;117(3):533–539. doi: 10.1164/arrd.1978.117.3.533. [DOI] [PubMed] [Google Scholar]
  9. Daniel T. M., Anderson P. A. The use of immunoabsorbents for the purification of mycobacterial antigens. J Lab Clin Med. 1977 Aug;90(2):354–360. [PubMed] [Google Scholar]
  10. Daniel T. M., DeMuth R. W. Immunochemical analyses of a major antigen of Mycobacterium szulgai. J Infect Dis. 1977 May;135(5):778–786. doi: 10.1093/infdis/135.5.778. [DOI] [PubMed] [Google Scholar]
  11. Daniel T. M., Ferguson L. E. Purification and Characterization of Two Proteins from Culture Filtrates of Mycobacterium tuberculosis H(37)Ra Strain. Infect Immun. 1970 Feb;1(2):164–168. doi: 10.1128/iai.1.2.164-168.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Daniel T. M., Good R. C., Janicki B. W. Immunoelectrophoresis of Mycobacterium tuberculosis antigens. Comparative analysis of cell extract and culture filtrate antigens. Am Rev Respir Dis. 1975 Nov;112(5):639–644. doi: 10.1164/arrd.1975.112.5.639. [DOI] [PubMed] [Google Scholar]
  13. Daniels T. M., Affronti L. F. Immunoelectrophoretic analysis of antigenic constituents of Seibert fractions of mycobacterial culture filtrates. Identification with the proposed United States-Japan reference nomenclature. Am Rev Respir Dis. 1973 Nov;108(5):1244–1248. doi: 10.1164/arrd.1973.108.5.1244. [DOI] [PubMed] [Google Scholar]
  14. Doroszczak N., Yoshida T., Cohen S. Subpopulations of lymphocytes to produce various lymphokines. I. Function of subpopulations separated by velocity sedimentation. J Immunol. 1977 Nov;119(5):1617–1620. [PubMed] [Google Scholar]
  15. Ellner J. J., Lipsky P. E., Rosenthal A. S. Antigen handling by guinea pig macrophages: further evidence for the sequestration of antigen relevant for activation of primed T lymphocytes. J Immunol. 1977 Jun;118(6):2053–2057. [PubMed] [Google Scholar]
  16. Janicki B. W., Chaparas S. D., Daniel T. M., Kubica G. P., Wright G. L., Yee G. S. A reference system for antigens of Mycobacterium tuberculosis. Am Rev Respir Dis. 1971 Oct;104(4):602–604. doi: 10.1164/arrd.1971.104.4.602. [DOI] [PubMed] [Google Scholar]
  17. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  18. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  19. Landi S., Held H. R., Tseng M. C. Disparity of potency between stabilized and nonstabilized dilute tuberculin solutions. Am Rev Respir Dis. 1971 Sep;104(3):385–393. doi: 10.1164/arrd.1971.104.3.385. [DOI] [PubMed] [Google Scholar]
  20. Sheffer A. L., Rocklin R. E., Goetzl E. J. Immunologic components of hypersensitivity reactions to cromolyn sodium. N Engl J Med. 1975 Dec 11;293(24):1220–1224. doi: 10.1056/NEJM197512112932402. [DOI] [PubMed] [Google Scholar]
  21. Spitler L., Benjamini E., Young J. D., Kaplan H., Fudenberg H. H. Studies on the immune response to a characterized antigenic determinant of the tobacco mosaic virus protein. J Exp Med. 1970 Jan 1;131(1):133–148. doi: 10.1084/jem.131.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Waterfield D., Levy J. G., Kilburn D. G., Teather R. M. The effect of haptenic peptides from performic acid oxidized ferredoxin from Clostridium pasteurianum and protein carrier-hapten conjugates on the immune response of macrophages and lymphoid cells from animals immunized against oxidized ferredoxin. Cell Immunol. 1972 Feb;3(2):253–263. doi: 10.1016/0008-8749(72)90164-5. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES