Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1979 Apr;24(1):121–126. doi: 10.1128/iai.24.1.121-126.1979

Development of antibodies reactive in antibody-dependent cellular cytotoxicity in infectious mononucleosis.

J F Hewetson, J F Böcker, P Sereni, C E Rapp, W Henle
PMCID: PMC414272  PMID: 222678

Abstract

Serial sera from patients with infectious mononucleosis were examined for the emergence of antibodies reactive in antibody-dependent cellular cytotoxicity tests, using Epstein-Barr virus-superinfected Raji cells as targets. For this specific purpose, the antibody-dependent cellular cytotoxicity test proved to be of limited sensitivity because only relatively high serum dilutions can be tested dependably, due to prozone effects at low serum concentrations, and because antibody-dependent cellular cytotoxicity reactions at the 5% level are not always statistically significant. Under the conditions of the test, antibody-dependent cellular cytotoxicity-reactive antibodies were not measurable, or only barely measurable, in early-acute-phase sera, but they became detectable during convalescence and increased thereafter, gradually over many months to the range of titers seen in healthy persons after long-past-primary Epstein-Barr virus infections. The percentages of antibody-dependent cellular cytotoxicity ultimately attained were on the order of 20% in most patients and healthy individuals, but in others did not exceed 10%. The likely identity of the antibodies reactive in the test with antibodies to late Epstein-Barr virus-determined cell membrane antigens has been discussed.

Full text

PDF
121

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ernberg I., Klein G., Kourilsky F. M., Silvestre D. Differentiation between early and late membrane antigen on human lymphoblastoid cell lines infected with Epstein-Barr virus. I. Immunofluorescence. J Natl Cancer Inst. 1974 Jul;53(1):61–65. doi: 10.1093/jnci/53.1.61. [DOI] [PubMed] [Google Scholar]
  2. Harada M., Pearson G., Pettigrew H., Redmon L., Orr T. Enhancement of normal lymphocyte cytotoxicity by sera with high antibody titers against H-2 or virus-associated antigens. Cancer Res. 1973 Nov;33(11):2886–2893. [PubMed] [Google Scholar]
  3. Henie G., Henle W., Horwitz C. A. Antibodies to Epstein-Barr virus-associated nuclear antigen in infectious mononucleosis. J Infect Dis. 1974 Sep;130(3):231–239. doi: 10.1093/infdis/130.3.231. [DOI] [PubMed] [Google Scholar]
  4. Henle G., Henle W. Immunofluorescence in cells derived from Burkitt's lymphoma. J Bacteriol. 1966 Mar;91(3):1248–1256. doi: 10.1128/jb.91.3.1248-1256.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Henle W., Henle G. E., Horwitz C. A. Epstein-Barr virus specific diagnostic tests in infectious mononucleosis. Hum Pathol. 1974 Sep;5(5):551–565. doi: 10.1016/s0046-8177(74)80006-7. [DOI] [PubMed] [Google Scholar]
  6. Henle W., Henle G., Zajac B. A., Pearson G., Waubke R., Scriba M. Differential reactivity of human serums with early antigens induced by Epstein-Barr virus. Science. 1970 Jul 10;169(3941):188–190. doi: 10.1126/science.169.3941.188. [DOI] [PubMed] [Google Scholar]
  7. Hewetson J. F., Rocchi G., Henle W., Henle G. Neutralizing antibodies to Epstein-Barr virus in healthy populations and patients with infectious mononucleosis. J Infect Dis. 1973 Sep;128(3):283–289. doi: 10.1093/infdis/128.3.283. [DOI] [PubMed] [Google Scholar]
  8. Jondal M. Antibody-dependent cellular cytotoxicity (ADCC) against Epstein-Barr virus-determined membrane antigens. I. Reactivity in sera from normal persons and from patients with acute infectious mononucleosis. Clin Exp Immunol. 1976 Jul;25(1):1–5. [PMC free article] [PubMed] [Google Scholar]
  9. Klein G., Pearson G., Henle G., Henle W., Diehl V., Niederman J. C. Relation between Epstein-- Barr viral and cell membrane immunofluorescence in Burkitt tumor cells. II. Comparison of cells and sera from patients with Burkitt's lymphoma and infectious mononucleosis. J Exp Med. 1968 Nov 1;128(5):1021–1030. doi: 10.1084/jem.128.5.1021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pearson G. R., Johansson B., Klein G. Antibody-dependent cellular cytotoxicity against Epstein-Barr virus-associated antigens in African patients with nasopharyngeal carcinoma. Int J Cancer. 1978 Aug 15;22(2):120–125. doi: 10.1002/ijc.2910220203. [DOI] [PubMed] [Google Scholar]
  11. Pearson G. R., Orr T. W. Antibody-dependent lymphocyte cytotoxicity against cells expressing Epstein-Barr virus antigens. J Natl Cancer Inst. 1976 Mar;56(3):485–488. doi: 10.1093/jnci/56.3.485. [DOI] [PubMed] [Google Scholar]
  12. Perlmann P., Holm G. Cytotoxic effects of lymphoid cells in vitro. Adv Immunol. 1969;11:117–193. doi: 10.1016/s0065-2776(08)60479-4. [DOI] [PubMed] [Google Scholar]
  13. Perlmann P., Perlmann H., Wigzell H. Lymphocyte mediated cytotoxicity in vitro. Induction and inhibition by humoral antibody and nature of effector cells. Transplant Rev. 1972;13:91–114. doi: 10.1111/j.1600-065x.1972.tb00061.x. [DOI] [PubMed] [Google Scholar]
  14. Reedman B. M., Klein G. Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines. Int J Cancer. 1973 May;11(3):499–520. doi: 10.1002/ijc.2910110302. [DOI] [PubMed] [Google Scholar]
  15. Rocchi G., Hewetson J. F. A practical and quantitative microtest for determination of neutralizing antibodies against Epstein-Barr virus. J Gen Virol. 1973 Mar;18(3):385–391. doi: 10.1099/0022-1317-18-3-385. [DOI] [PubMed] [Google Scholar]
  16. Silvestre D., Ernberg I., Neauport-Sautes C., Kourilsky F. M., Klein G. Differentiation between early and late membrane antigen on human lymphoblastoid cell lines infected with Epstein-Barr virus. II. Immunoelectron microscopy. J Natl Cancer Inst. 1974 Jul;53(1):67–74. doi: 10.1093/jnci/53.1.67. [DOI] [PubMed] [Google Scholar]
  17. Svedmyr E., Jondal M. Cytotoxic effector cells specific for B Cell lines transformed by Epstein-Barr virus are present in patients with infectious mononucleosis. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1622–1626. doi: 10.1073/pnas.72.4.1622. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES