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. 1979 Apr;24(1):202–210. doi: 10.1128/iai.24.1.202-210.1979

Immunoglobulin G from subacute sclerosing panencephalitis brain as an immunological reagent.

M L Weil, E Norrby, H H Itabashi, R Wilson, W W Tourtellotte, D C Heiner
PMCID: PMC414284  PMID: 457270

Abstract

Proteins were extracted at pH 2.5 from carefully washed brain homogenates of patients with subacute sclerosing panencephalitis. Immunoglobulin G was isolated from the extracts by gel filtration and immunoadsorbent chromatography and was 125I radioiodinated. The labeled immunoglobulin G was reacted with purified measles virus antigens by crossed immunoelectrophoresis and utilized to identify antigens in measles-infected cell cultures and hamster brain tissue subjected to treatment corresponding to that used for elution of immunoglobulin G. The method described is useful in identifying the specific nature of antibodies as well as antigens in tissue-associated immune complexes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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