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. 1979 May;24(2):363–370. doi: 10.1128/iai.24.2.363-370.1979

Antigenic differences between extracts of actively replicating and synchronized resting cells of Mycobacterium tuberculosis.

L G Wayne, H A Sramek
PMCID: PMC414310  PMID: 110697

Abstract

Protein extracts were prepared from aerobically replicating cells of Mycobacterium tuberculosis and from synchronized non-replicating cells derived from the sediments of non-agitated cultures. Although both preparations share a number of antigens, the extracts of the non-replicating cells also contain antigenic components that are not shared by the replicating cells, and which can be isolated and visualized by immunoaffinity chromatography and immunoelectrophoretic techniques.

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Selected References

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  1. Anderson N. G., Willis D. D., Holladay D. W., Caton J. E., Holleman J. W., Eveleigh J. W., Attrill J. E., Ball F. L., Anderson N. L. Analytical techniques for cell fractions. XX. Cyclic affinity chromatography: principles and applications. Anal Biochem. 1975 Oct;68(2):371–393. doi: 10.1016/0003-2697(75)90634-x. [DOI] [PubMed] [Google Scholar]
  2. Antoine A. D., Tepper B. S. Environmental control of glycogen and lipid content of Mycobacterium tuberculosis. J Bacteriol. 1969 Oct;100(1):538–539. doi: 10.1128/jb.100.1.538-539.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Crowle A. J., Cline L. J. An improved stain for immunodiffusion tests. J Immunol Methods. 1977;17(3-4):379–381. doi: 10.1016/0022-1759(77)90122-3. [DOI] [PubMed] [Google Scholar]
  4. Daniel T. M., Anderson P. A. The isolation by immunoabsorbent affinity chromatography and physicochemical characterization of Mycobacterium tuberculosis antigen 5. Am Rev Respir Dis. 1978 Mar;117(3):533–539. doi: 10.1164/arrd.1978.117.3.533. [DOI] [PubMed] [Google Scholar]
  5. Daniel T. M., Anderson P. A. The use of immunoabsorbents for the purification of mycobacterial antigens. J Lab Clin Med. 1977 Aug;90(2):354–360. [PubMed] [Google Scholar]
  6. Daniel T. M., Good R. C., Janicki B. W. Immunoelectrophoresis of Mycobacterium tuberculosis antigens. Comparative analysis of cell extract and culture filtrate antigens. Am Rev Respir Dis. 1975 Nov;112(5):639–644. doi: 10.1164/arrd.1975.112.5.639. [DOI] [PubMed] [Google Scholar]
  7. Daniel T. M., Janicki B. W. Mycobacterial antigens: a review of their isolation, chemistry, and immunological properties. Microbiol Rev. 1978 Mar;42(1):84–113. doi: 10.1128/mr.42.1.84-113.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Diaz G. A., Wayne L. G. Isolation and characterization of catalase produced by Mycobacterium tuberculosis. Am Rev Respir Dis. 1974 Sep;110(3):312–319. doi: 10.1164/arrd.1974.110.3.312. [DOI] [PubMed] [Google Scholar]
  9. Ferguson H. R., McClatchy J. K., Sharpton T. R., Minden P. Immunological method to differentiate between antigens of tubercle bacilli, other mycobacterial species, and non-acid-fast bacteria. Infect Immun. 1978 Oct;22(1):101–106. doi: 10.1128/iai.22.1.101-106.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Harboe M., Closs O., Deverill J. Production of monospecific antisera against antigenic componentsof Mycobacterium bovis (BCG). Scand J Immunol. 1976;5(6-7):861–866. doi: 10.1111/j.1365-3083.1976.tb03035.x. [DOI] [PubMed] [Google Scholar]
  11. Harboe M., Closs O., Svindahl K., Deverill J. Production and assay of antibodies against one antigenic component of Mycobacterium bovis BCG. Infect Immun. 1977 May;16(2):662–672. doi: 10.1128/iai.16.2.662-672.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Huppert M., Spratt N. S., Vukovich K. R., Sun S. H., Rice E. H. Antigenic analysis of coccidioidin and spherulin determined by two-dimensional immunoelectrophoresis. Infect Immun. 1978 May;20(2):541–551. doi: 10.1128/iai.20.2.541-551.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Janicki B. W., Chaparas S. D., Daniel T. M., Kubica G. P., Wright G. L., Yee G. S. A reference system for antigens of Mycobacterium tuberculosis. Am Rev Respir Dis. 1971 Oct;104(4):602–604. doi: 10.1164/arrd.1971.104.4.602. [DOI] [PubMed] [Google Scholar]
  14. Janicki B. W., Wright G. L., Jr, Good R. C., Chaparas S. D. Comparison of antigens in sonic and pressure cell extracts of Mycobacterium tuberculosis. Infect Immun. 1976 Feb;13(2):425–437. doi: 10.1128/iai.13.2.425-437.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Turcotte R. The variations in the antigenic composition of Mycobacterium tuberculosis during the growth cycle as measured by the passive hemagglutination and precipitation reactions. Can J Microbiol. 1969 Jul;15(7):681–688. doi: 10.1139/m69-121. [DOI] [PubMed] [Google Scholar]
  16. Turcotte R. Yield of non-dialyzable mycobacterial constituents during the growth cycle. Can J Microbiol. 1969 Jan;15(1):35–41. doi: 10.1139/m69-006. [DOI] [PubMed] [Google Scholar]
  17. Wayne L. G., Diaz G. A. Autolysis and secondary growth of Mycobacterium tuberculosis in submerged culture. J Bacteriol. 1967 Apr;93(4):1374–1381. doi: 10.1128/jb.93.4.1374-1381.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wayne L. G. Dynamics of submerged growth of Mycobacterium tuberculosis under aerobic and microaerophilic conditions. Am Rev Respir Dis. 1976 Oct;114(4):807–811. doi: 10.1164/arrd.1976.114.4.807. [DOI] [PubMed] [Google Scholar]
  19. Wayne L. G. Synchronized replication of Mycobacterium tuberculosis. Infect Immun. 1977 Sep;17(3):528–530. doi: 10.1128/iai.17.3.528-530.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Zoller M., Matzku S. Antigen and antibody purification by immunoadsorption: elimination of non-biospecifically bound proteins. J Immunol Methods. 1976;11(3-4):287–295. doi: 10.1016/0022-1759(76)90122-8. [DOI] [PubMed] [Google Scholar]

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