Extended Data Figure 7. Signature anatomical and physiological characteristics of GFP tagged On-Off DSGCs.

Flat mount retina with a, GFP⁺ On-Off DSGCs. b, co-stained with dapi. c, Positions of GFP⁺ RGCs. Scale in c, 150 µm. d–f, High magnification views. Scale, 12 µm. g, Targeted fill of a GFP⁺ DSGC. Scale, 50 µm. h, Schematic of On-Off DSGC stratification and starburst amacrine cells (magenta). Labeling as in Extended Fig. 1. i,j, Higher magnification of framed region in g stained for VAChT (starburst amacrine processes). Asterisk: ‘looping arborizations’. Dashed line: GFP arbor, which matches VAChT plexus. Scale, 10 µm. k,l, Side (x-z plane) views of cell in (g). GFP⁺ dendrites co-stratify with both the On and Off sublayers. Scale, 5 µm. m, Direction-tuned response of a GFP⁺ On-Off DSGC targeted for recording and receptive field characterization. The spike count is highest for bars moving toward ~270° in the cardinal axes.