Abstract
A microtiter technique was used to measure lymphocyte stimulation by antigen-pulsed monocytes. Monocytes from human blood were isolated on polyester film disks. The disks were exposed to antigen and incubated together with lymphocytes in the wells of a microtiter plate. The method was technically simple and required no expensive materials.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cline M. J., Swett V. C. The interaction of human monocytes and lymphocytes. J Exp Med. 1968 Dec 1;128(6):1309–1325. doi: 10.1084/jem.128.6.1309. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hanifin J. M., Cline M. J. Human monocytes and macrophages. Interaction with antigen and lymphocytes. J Cell Biol. 1970 Jul;46(1):97–105. doi: 10.1083/jcb.46.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartzman R. J., Bach M. L., Bach F. H., Thurman G. B., Sell K. W. Precipitation of radioactively labeled samples: a semi-automatic multiple-sample processor. Cell Immunol. 1972 Jun;4(2):182–186. doi: 10.1016/0008-8749(72)90018-4. [DOI] [PubMed] [Google Scholar]
- Hersh E. M., Harris J. E. Macrophage-lymphocyte interaction in the antigen-induced blastogenic response of human peripheral blood leukocytes. J Immunol. 1968 Jun;100(6):1184–1194. [PubMed] [Google Scholar]
- KUPER S. W., BIGNALL J. R., LUCKCOCK E. D. A quantitative method for studying tumour cells in blood. Lancet. 1961 Apr 22;1(7182):852–853. doi: 10.1016/s0140-6736(61)90177-5. [DOI] [PubMed] [Google Scholar]
- Lipsky P. E., Rosenthal A. S. Macrophage-lymphocyte interaction. II. Antigen-mediated physical interactions between immune guinea pig lymph node lymphocytes and syngeneic macrophages. J Exp Med. 1975 Jan 1;141(1):138–154. doi: 10.1084/jem.141.1.138. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oppenheim J. J., Rosenstreich D. L. Signals regulating in vitro activation of lymphocytes. Prog Allergy. 1976;20:65–194. doi: 10.1159/000398282. [DOI] [PubMed] [Google Scholar]
- RABINOWITZ Y. SEPARATION OF LYMPHOCYTES, POLYMORPHONUCLEAR LEUKOCYTES AND MONOCYTES ON GLASS COLUMNS, INCLUDING TISSUE CULTURE OBSERVATIONS. Blood. 1964 Jun;23:811–828. [PubMed] [Google Scholar]
- Rosenthal A. S., Barcinski M. A., Rosenwasser L. J. Function of macrophages in genetic control of immune responsiveness. Fed Proc. 1978 Jan;37(1):79–85. [PubMed] [Google Scholar]
- Seeger R. C., Oppenheim J. J. Synergistic interaction of macrophages and lymphocytes in antigen-induced transformation of lymphocytes. J Exp Med. 1970 Jul 1;132(1):44–65. doi: 10.1084/jem.132.1.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tärnvik A., Holm S. E. Stimulation of subpopulations of human lymphocytes by a vaccine strain of Francisella tularensis. Infect Immun. 1978 Jun;20(3):698–704. doi: 10.1128/iai.20.3.698-704.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]