Abstract
Human peripheral blood leukocytes (PBL) were examined for their ability to support growth of several group A arboviruses in vitro. Cells were refractory to infection with eastern (EEE) and western (WEE) equine encephalitis viruses, whereas Venezuelan equine encephalomyelitis (VEE) virus was shown to infect and replicate to a substantially high titer. When PBL were fractionated into purified subpopulations, only the monocytes were susceptible to predictive VEE virus infection. Lymphocytes treated 24 h before virus inoculation with phytohemagglutinin (10 microgram/ml) were capable of propagating significant amounts of VEE virus. A monocytic cell line, J-111, was also susceptible to infection with VEE, EEE, and WEE viruses, whereas a lymphocytic cell line, Raji, was refractory. Additional information on the participation of PBL during human infection with these viruses may add considerably to our understanding of their differing pathogenicities and clinical pictures.
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- BLACK L., BERENBAUM M. C. FACTORS AFFECTING THE DYE EXCLUSION TEST FOR CELL VIABILITY. Exp Cell Res. 1964 Jun;35:9–13. doi: 10.1016/0014-4827(64)90066-7. [DOI] [PubMed] [Google Scholar]
- DULBECCO R., VOGT M. One-step growth curve of Western equine encephalomyelitis virus on chicken embryo cells grown in vitro and analysis of virus yields from single cells. J Exp Med. 1954 Feb;99(2):183–199. doi: 10.1084/jem.99.2.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Duc-Nguyen H., Henle W. Replication of mumps virus in human leukocyte cultures. J Bacteriol. 1966 Jul;92(1):258–265. doi: 10.1128/jb.92.1.258-265.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edelman R., Wheelock E. F. Specific role of each human leukocyte type in viral infections. I. Monocyte as host cell for vesicular stomatitis virus replication in vitro. J Virol. 1967 Dec;1(6):1139–1149. doi: 10.1128/jvi.1.6.1139-1149.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edelman R., Wheelock E. F. Specific role of each human leukocyte type in viral infections. II. Phytohemagglutinin-treated lymphocytes as host cells for vesicular stomatitis virus replication in vitro. J Virol. 1968 May;2(5):440–448. doi: 10.1128/jvi.2.5.440-448.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edelman R., Wheelock E. F. Vesicular stomatitis virus replication in human leukocyte cultures: enhancement by phytohemagglutinin. Science. 1966 Nov 25;154(3752):1053–1055. doi: 10.1126/science.154.3752.1053. [DOI] [PubMed] [Google Scholar]
- Ehrenkranz N. J., Ventura A. K. Venezuelan equine encephalitis virus infection in man. Annu Rev Med. 1974;25:9–14. doi: 10.1146/annurev.me.25.020174.000301. [DOI] [PubMed] [Google Scholar]
- GRESSER I., CHANY C. MULTIPLICATION OF POLIOVIRUS TYPE I IN PREPARATIONS OF HUMAN LEUKOCYTES AND ITS INHIBITION BY INTERFERON. J Immunol. 1964 Jun;92:889–895. [PubMed] [Google Scholar]
- Halstead S. B., Chow J. S., Marchette N. J. Immunological enhancement of dengue virus replication. Nat New Biol. 1973 May 2;243(122):24–26. [PubMed] [Google Scholar]
- Halstead S. B., Marchette N. J., Sung Chow J. S., Lolekha S. Dengue virus replication enhancement in peripheral blood leukocytes from immune human beings. Proc Soc Exp Biol Med. 1976 Jan;151(1):136–139. doi: 10.3181/00379727-151-39160. [DOI] [PubMed] [Google Scholar]
- Halstead S. B., Shotwell H., Casals J. Studies on the pathogenesis of dengue infection in monkeys. II. Clinical laboratory responses to heterologous infection. J Infect Dis. 1973 Jul;128(1):15–22. doi: 10.1093/infdis/128.1.15. [DOI] [PubMed] [Google Scholar]
- JAHMIAS A. J., KIBRICK S., ROSAN R. C. VIRAL LEUKOCYTE INTERRELATIONSHIPS. I. MULTIPLICATION OF A DNA VIRUS--HERPES SIMPLEX--IN HUMAN LEUKOCYTE CULTURES. J Immunol. 1964 Jul;93:69–74. [PubMed] [Google Scholar]
- Joseph B. S., Lampert P. W., Oldstone M. B. Replication and persistence of measles virus in defined subpopulations of human leukocytes. J Virol. 1975 Dec;16(6):1638–1649. doi: 10.1128/jvi.16.6.1638-1649.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaplan J., Shope T. C., Peterson W. D., Jr Epstein-barr virus-negative human malignant T-cell lines. J Exp Med. 1974 May 1;139(5):1070–1076. doi: 10.1084/jem.139.5.1070. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marchette N. J., Halstead S. B., Chow J. S. Replication of dengue viruses in cultures of peripheral blood leukocytes from dengue-immune rhesus monkeys. J Infect Dis. 1976 Mar;133(3):274–282. doi: 10.1093/infdis/133.3.274. [DOI] [PubMed] [Google Scholar]
- Olding L. B., Jensen F. C., Oldstone M. B. Pathogenesis of of cytomegalovirus infection. I. Activation of virus from bone marrow-derived lymphocytes by in vitro allogenic reaction. J Exp Med. 1975 Mar 1;141(3):561–572. doi: 10.1084/jem.141.3.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roca-Garcia M., Jungherr E. L., Johnson H. N., Cox H. R. An attenuated strain of Western equine encephalitis virus as a possible live immunizing agent. Proc Annu Meet U S Anim Health Assoc. 1964;68:24–40. [PubMed] [Google Scholar]
- Theofilopoulos A. N., Brandt W. E., Russell P. K., Dixon F. T. Replication of dengue-2 virus in cultured human lymphoblastoid cells and subpopulations of human peripheral leukocytes. J Immunol. 1976 Sep;117(3):953–961. [PubMed] [Google Scholar]
- Wheelock E. F., Edelman R. Specific role of each human leukocyte type in viral infections. 3. 17D yellow fever virus replication and interferon production in homogeneous leukocyte cultures treated with phytohemagglutinin. J Immunol. 1969 Sep;103(3):429–436. [PubMed] [Google Scholar]