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. 1979 Jun;24(3):647–655. doi: 10.1128/iai.24.3.647-655.1979

Depression of Rauscher leukemia virus envelope glycoprotein gp71 binding by lymphoid cells during leukemogenesis in mice.

A K Fowler, C D Reed, C W Riggs, D R Twardzik, O S Weislow, A Hellman
PMCID: PMC414355  PMID: 468372

Abstract

The availability of membrane receptors for the 71,000-dalton envelope glycoprotein (gp71) of Rauscher murine leukemia virus on splenic and thymic cells from BALB/c mice during Rauscher murine leukemia virus-induced leukemogenesis was determined utilizing a radiolabeled gp71 binding assay. Shortly after infection, the relative cellular [125I]gp71 binding level decreased, first with splenic cells (at day 7 to 10 after infection) and later with thymic cells (at day 10 to 20 after infection). The dependency of the reduction of binding on the replication of the inoculated virus was demonstrated by regression analyses using cellular gp71 binding level as the dependent variable and infectious virus titer, as well as viral gp71 and p30 levels, of spleens and thymuses from infected mice as independent variables. With each independent variable, the reduction of gp71 binding for both cell types was highly dependent (P less than 0.01) on the level of virus detected in their respective organ. In the early stages of leukemogenesis, the [125I]gp71 binding level declined to approximately 20 to 30% of control values. During this period the rate of reduction of binding was very rapid and, in general was similar for both splenic and thymic cells. Further progression of the disease resulted in little or no further reduction in binding. The application of this technique to monitor host ecotropic virus synthesis and to study cell surface virus receptor control mechanisms in vivo is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aoki T., Boyse E. A., Old L. J. Wild-type Gross leukemia virus. 3. Serological tests as indicators of leukemia risk. J Natl Cancer Inst. 1968 Jul;41(1):103–110. [PubMed] [Google Scholar]
  2. Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
  3. Besmer P., Baltimore D. Mechanism of restriction of ecotropic and xenotropic murine leukemia viruses and formation of pseudotypes between the two viruses. J Virol. 1977 Mar;21(3):965–973. doi: 10.1128/jvi.21.3.965-973.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Callahan R., Benveniste R. E., Lieber M. M., Todaro G. J. Nucleic acid homology of murine type-C viral genes. J Virol. 1974 Dec;14(6):1394–1403. doi: 10.1128/jvi.14.6.1394-1403.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cloyd M. W., Bolognesi D. P., Bigner D. D. Immunofluorescent analysis of expression of the RNA tumor virus major glycoprotein, gp71, on surfaces of virus-producing murine and other mammalian species cell lines. Cancer Res. 1977 Mar;37(3):922–930. [PubMed] [Google Scholar]
  6. De Larco J. E., Rapp U. R., Todaro G. J. Cell surface receptors for ecotropic MuLV: detection and tissue distributions of free receptors in vivo. Int J Cancer. 1978 Mar 15;21(3):356–360. doi: 10.1002/ijc.2910210317. [DOI] [PubMed] [Google Scholar]
  7. DeLarco J., Todaro G. J. Membrane receptors for murine leukemia viruses: characterization using the purified viral envelope glycoprotein, gp71. Cell. 1976 Jul;8(3):365–371. doi: 10.1016/0092-8674(76)90148-3. [DOI] [PubMed] [Google Scholar]
  8. Fischinger P. J., Nomura S., Bolognesi D. P. A novel murine oncornavirus with dual eco- and xenotropic properties. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5150–5155. doi: 10.1073/pnas.72.12.5150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fowler A. K., Kouttab N. M., Kind P. D., Strickland J. E., Hellman A. Oncornaviral protein modulation in mouse uterine tissue by estrogen (38467). Proc Soc Exp Biol Med. 1975 Jan;148(1):14–18. doi: 10.3181/00379727-148-38467. [DOI] [PubMed] [Google Scholar]
  10. Fowler A. K., Twardzik D. R., Reed C. D., Weislow O. S., Hellman A. Binding characteristics of Rauscher leukemia virus envelope glycoprotein gp71 to murine lymphoid cells. J Virol. 1977 Dec;24(3):729–735. doi: 10.1128/jvi.24.3.729-735.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gelb L. D., Milstien J. B., Martin M. A., Aaronson S. A. Characterization of murine leukaemia virus-specific DNA present in normal mouse cells. Nat New Biol. 1973 Jul 18;244(133):76–79. doi: 10.1038/newbio244076a0. [DOI] [PubMed] [Google Scholar]
  13. Hartley J. W., Rowe W. P., Huebner R. J. Host-range restrictions of murine leukemia viruses in mouse embryo cell cultures. J Virol. 1970 Feb;5(2):221–225. doi: 10.1128/jvi.5.2.221-225.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hartley J. W., Rowe W. P. Naturally occurring murine leukemia viruses in wild mice: characterization of a new "amphotropic" class. J Virol. 1976 Jul;19(1):19–25. doi: 10.1128/jvi.19.1.19-25.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hunsmann G., Moennig V., Pister L., Seifert E., Schäfer W. Properties of mouse leukemia viruses. VIII. The major viral glycoprotein of Friend leukemia virus. Seroimmunological, interfering and hemagglutinating capacities. Virology. 1974 Dec;62(2):307–318. doi: 10.1016/0042-6822(74)90394-8. [DOI] [PubMed] [Google Scholar]
  16. Lesniak M. A., Roth J. Regulation of receptor concentration by homologous hormone. Effect of human growth hormone on its receptor in IM-9 lymphocytes. J Biol Chem. 1976 Jun 25;251(12):3720–3729. [PubMed] [Google Scholar]
  17. Levy J. A. Xenotropic viruses: murine leukemia viruses associated with NIH Swiss, NZB, and other mouse strains. Science. 1973 Dec 14;182(4117):1151–1153. doi: 10.1126/science.182.4117.1151. [DOI] [PubMed] [Google Scholar]
  18. Lowy D. R., Chattopadhyay S. K., Teich N. M., Rowe W. P., Levine A. S. AKR murine leukemia virus genome: frequency of sequences in DNA of high-, low-, and non-virus-yielding mouse strains. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3555–3559. doi: 10.1073/pnas.71.9.3555. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Myers D. D., Meier H., Huebner R. J. Prevalence of murine C-type RNA virus group specific antigen in inbred strains of mice. Life Sci II. 1970 Sep 22;9(18):1071–1080. doi: 10.1016/0024-3205(70)90016-0. [DOI] [PubMed] [Google Scholar]
  20. Nexø B. A., Krog H. H. C-type virus protein p30 in blood from inbred mice correlates with their later incidence of leukemia. Infect Immun. 1977 Feb;15(2):376–381. doi: 10.1128/iai.15.2.376-381.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Nowinski R. C., Doyle T. Cellular changes in the thymuses of preleukemic AKR mice: correlation with changes in the expression of murine leukemia viruses. Cell. 1977 Oct;12(2):341–353. doi: 10.1016/0092-8674(77)90110-6. [DOI] [PubMed] [Google Scholar]
  22. Pincus T., Hartley J. W., Rowe W. P. A major genetic locus affecting resistance to infection with murine leukemia viruses. I. Tissue culture studies of naturally occurring viruses. J Exp Med. 1971 Jun 1;133(6):1219–1233. doi: 10.1084/jem.133.6.1219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. RAUSCHER F. J. A virus-induced disease of mice characterized by erythrocytopoiesis and lymphoid leukemia. J Natl Cancer Inst. 1962 Sep;29:515–543. [PubMed] [Google Scholar]
  24. Rowe W. P., Pincus T. Quantitative studies of naturally occurring murine leukemia virus infection of AKR mice. J Exp Med. 1972 Feb 1;135(2):429–436. doi: 10.1084/jem.135.2.429. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rowe W. P. Studies of genetic transmission of murine leukemia virus by AKR mice. I. Crosses with Fv-1 n strains of mice. J Exp Med. 1972 Nov 1;136(5):1272–1285. doi: 10.1084/jem.136.5.1272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Sarma P. S., Cheong M. P., Hartley J. W., Huebner R. J. A viral interference test for mouse leukemia viruses. Virology. 1967 Sep;33(1):180–184. doi: 10.1016/0042-6822(67)90111-0. [DOI] [PubMed] [Google Scholar]
  27. Schäfer W., Fischinger P. J., Collins J. J., Bolognesi D. P. Role of carbohydrate in biological functions of Friend murine leukemia virus gp71. J Virol. 1977 Jan;21(1):35–40. doi: 10.1128/jvi.21.1.35-40.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Steeves R. A., Strand M., August J. T. Structural proteins of mammalian oncogenic RNA viruses: murine leukemia virus neutralization by antisera prepared against purified envelope glycoprotein. J Virol. 1974 Jul;14(1):187–189. doi: 10.1128/jvi.14.1.187-189.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Stephenson J. R., Aaronson S. A. Genetic factors influencing C-type RNA virus induction. J Exp Med. 1972 Jul 1;136(1):175–184. doi: 10.1084/jem.136.1.175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Strand M., August J. T. Structural proteins of mammalian oncogenic RNA viruses: multiple antigenic determinants of the major internal protein and envelope glycoprotein. J Virol. 1974 Jan;13(1):171–180. doi: 10.1128/jvi.13.1.171-180.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Strand M., Lilly F., August J. T. Host control of endogenous murine leukemia virus gene expression: concentrations of viral proteins in high and low leukemia mouse strains. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3682–3686. doi: 10.1073/pnas.71.9.3682. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Taylor B. A., Meier H., Myers D. D. Host-gene control of C-type RNA tumor virus: inheritance of the group-specific antigen of murine leukemia virus. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3190–3194. doi: 10.1073/pnas.68.12.3190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Ulrich K., Nexo J. A. Virus protein p30 in blood predicts development of leukaemia in mice injected with MuLV. Nature. 1977 Jun 23;267(5613):723–724. doi: 10.1038/267723a0. [DOI] [PubMed] [Google Scholar]

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