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. 1979 Jun;24(3):793–797. doi: 10.1128/iai.24.3.793-797.1979

Heat-labile enterotoxin production in isolates from a shipboard outbreak of human diarrheal illness.

K Wachsmuth, J Wells, P Shipley, R Ryder
PMCID: PMC414376  PMID: 381200

Abstract

As reported elsewhere, an enterotoxigenic strain of Escherichia coli serotype O25:K98:NM was epidemiologically incriminated as the etiological agent in a shipboard outbreak of diarrheal illness. This enterotoxigenic E. coli strain and possibly other enteric isolates were found to produce heat-labile toxin and not heat-stable toxin. Since previous genetic analyses of enterotoxigenic E. coli strains producing heat-labile and heat-stable toxins have shown a plasmid location for both toxin determinants and since in this outbreak more than one bacterial strain appeared to produce only heat-labile toxin, the possibility of an extrachromosomal heat-labile toxin determinant was investigated. Results of endonuclease cleavage and hybridization experiments, as well as apparent heat-labile toxin phenotypic instability, strongly suggest a plasmid mediation of toxin production. Additionally, the stability of this heat-labile toxin production was evaluated after several traditional methods of bacterial cell preservation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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