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. 1979 Jun;24(3):906–911. doi: 10.1128/iai.24.3.906-911.1979

Electron microscopic studies on the attachment of Mycoplasma pneumoniae to guinea pig erythrocytes.

H Brunner, H Krauss, H Schaar, H G Schiefer
PMCID: PMC414393  PMID: 468379

Abstract

A mechanism of pathogenicity of Mycoplasma pneumoniae is its ability to attach to the surface of mammalian cells. It has previously been demonstrated by others that M. pneumoniae adheres with a specialized terminal structure, the "tip," to ciliated epithelial cells of the respiratory tract. In this report we show by electron microscopy that M. pneumoniae adsorbs with membrane sites other than the tip to guinea pig erythrocytes.

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Selected References

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  1. Biberfeld G., Biberfeld P. Ultrastructural features of Mycoplasma pneumoniae. J Bacteriol. 1970 Jun;102(3):855–861. doi: 10.1128/jb.102.3.855-861.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bredt W. Motility and multiplication of Mycoplasma pneumoniae. A phase contrast study. Pathol Microbiol (Basel) 1968;32(6):321–326. doi: 10.1159/000162074. [DOI] [PubMed] [Google Scholar]
  3. Brunner H., Schaeg W., Brück U., Schummer U., Schiefer H. G. A staphylococcal radioimmunoassay for detection of antibodies to Mycoplasma pneumoniae. Med Microbiol Immunol. 1977 May 18;163(1):25–35. doi: 10.1007/BF02126706. [DOI] [PubMed] [Google Scholar]
  4. CHANOCK R. M., HAYFLICK L., BARILE M. F. Growth on artificial medium of an agent associated with atypical pneumonia and its identification as a PPLO. Proc Natl Acad Sci U S A. 1962 Jan 15;48:41–49. doi: 10.1073/pnas.48.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chanock R. M. Mycoplasma infections of man. N Engl J Med. 1965 Dec 2;273(23):1257–concl. doi: 10.1056/NEJM196512022732307. [DOI] [PubMed] [Google Scholar]
  6. Cherry J. D., Taylor-Robinson D. Growth and Pathogenesis of Mycoplasma mycoides var. capri in Chicken Embryo Tracheal Organ Cultures. Infect Immun. 1970 Oct;2(4):431–438. doi: 10.1128/iai.2.4.431-438.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cherry J. D., Taylor-Robinson D. Growth and pathogenicity studies of Mycoplasma gallisepticum in chicken tracheal organ cultures. J Med Microbiol. 1971 Nov;4(4):441–449. doi: 10.1099/00222615-4-4-441. [DOI] [PubMed] [Google Scholar]
  8. Cohen G., Somerson N. L. Mycoplasma pneumoniae: hydrogen peroxide secretion and its possible role in virulence. Ann N Y Acad Sci. 1967 Jul 28;143(1):85–87. doi: 10.1111/j.1749-6632.1967.tb27648.x. [DOI] [PubMed] [Google Scholar]
  9. Collier A. M., Clyde W. A., Jr Appearance of Mycoplasma pneumoniae in lungs of experimentally infected hamsters and sputum from patients with natural disease. Am Rev Respir Dis. 1974 Dec;110(6):765–773. doi: 10.1164/arrd.1974.110.6P1.765. [DOI] [PubMed] [Google Scholar]
  10. Collier A. M., Clyde W. A., Jr, Denny F. W. Biologic effects of Mycoplasma pneumoniae and other mycoplasmas from man on hamster tracheal organ culture. Proc Soc Exp Biol Med. 1969 Dec;132(3):1153–1158. doi: 10.3181/00379727-132-34385. [DOI] [PubMed] [Google Scholar]
  11. Collier A. M., Clyde W. A., Jr, Denny F. W. Mycoplasma pneumoniae in hamster tracheal organ culture: immunofluorescent and electron microscopic studies. Proc Soc Exp Biol Med. 1971 Feb;136(2):569–573. doi: 10.3181/00379727-136-35313. [DOI] [PubMed] [Google Scholar]
  12. Collier A. M., Clyde W. A. Relationships Between Mycoplasma pneumoniae and Human Respiratory Epithelium. Infect Immun. 1971 May;3(5):694–701. doi: 10.1128/iai.3.5.694-701.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gabridge M. G., Barden-Stahl Y. D., Polisky R. B., Engelhardt J. A. Differences in the attachment of Mycoplasma pneumoniae cells and membranes to tracheal epithelium. Infect Immun. 1977 Jun;16(3):766–772. doi: 10.1128/iai.16.3.766-772.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Gesner B., Thomas L. Sialic acid binding sites: role in hemagglutination by Mycoplasma gallisepticum. Science. 1966 Feb 4;151(3710):590–591. doi: 10.1126/science.151.3710.590. [DOI] [PubMed] [Google Scholar]
  15. Goel M. C., Lemcke R. M. Dissociation of Mycoplasma gallisepticum membranes with lithium diiodosalicylate and isolation of glycoprotein. Ann Microbiol (Paris) 1975 Oct-Nov;126(3):299–312. [PubMed] [Google Scholar]
  16. Hu P. C., Collier A. M., Baseman J. B. Surface parasitism by Mycoplasma pneumoniae of respiratory epithelium. J Exp Med. 1977 May 1;145(5):1328–1343. doi: 10.1084/jem.145.5.1328. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Huang R. T. Adsorption of influenza virus to charged groups on natural and artificial surfaces. Med Microbiol Immunol. 1974;159(2):129–135. doi: 10.1007/BF02123725. [DOI] [PubMed] [Google Scholar]
  18. Kahane I., Brunner H. Isolation of a glycoprotein from Mycoplasma pneumoniae membranes. Infect Immun. 1977 Nov;18(2):273–277. doi: 10.1128/iai.18.2.273-277.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lipman R. P., Clyde W. A., Jr The interrelationship of virulence, cytadsorption, and peroxide formation in Mycoplasma pneumoniae. Proc Soc Exp Biol Med. 1969 Sep;131(4):1163–1167. doi: 10.3181/00379727-131-34061. [DOI] [PubMed] [Google Scholar]
  20. Maisel J. C., Babbitt L. H., John T. J. Fatal Mycoplasma pneumoniae infection with isolation of organisms from lung. JAMA. 1967 Oct 23;202(4):287–290. [PubMed] [Google Scholar]
  21. Manchee R. J., Taylor-Robinson D. Studies on the nature of receptors involved in attachment of tissue culture cells to mycoplasmas. Br J Exp Pathol. 1969 Feb;50(1):66–75. [PMC free article] [PubMed] [Google Scholar]
  22. Powell D. A., Hu P. C., Wilson M., Collier A. M., Baseman J. B. Attachment of Mycoplasma pneumoniae to respiratory epithelium. Infect Immun. 1976 Mar;13(3):959–966. doi: 10.1128/iai.13.3.959-966.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schiefer H. G., Gerhardt U., Brunner H., Krüpe M. Studies with lectins on the surface carbohydrate structures of mycoplasma membranes. J Bacteriol. 1974 Oct;120(1):81–88. doi: 10.1128/jb.120.1.81-88.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Schiefer H. G., Krauss H., Brunner H., Gerhardt U. Ultrastructural visualization of anionic sites on mycoplasma membranes by polycationic ferritin. J Bacteriol. 1976 Jul;127(1):461–468. doi: 10.1128/jb.127.1.461-468.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sobeslavsky O., Prescott B., Chanock R. M. Adsorption of Mycoplasma pneumoniae to neuraminic acid receptors of various cells and possible role in virulence. J Bacteriol. 1968 Sep;96(3):695–705. doi: 10.1128/jb.96.3.695-705.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Somerson N. L., James W. D., Walls B. E., Chanock R. M. Growth of Mycoplasma pneumoniae on a glass surface. Ann N Y Acad Sci. 1967 Jul 28;143(1):384–389. doi: 10.1111/j.1749-6632.1967.tb27680.x. [DOI] [PubMed] [Google Scholar]
  27. Somerson N. L., Walls B. E., Chanock R. M. Hemolysin of Mycoplasma pneumoniae: tentative identification as a peroxide. Science. 1965 Oct 8;150(3693):226–228. doi: 10.1126/science.150.3693.226. [DOI] [PubMed] [Google Scholar]
  28. Taylor-Robinson D., Manchee R. J. Adherence of mycoplasmas to glass and plastic. J Bacteriol. 1967 Nov;94(5):1781–1782. doi: 10.1128/jb.94.5.1781-1782.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Taylor-Robinson D., Manchee R. J. Novel approach to studying relationships between mycoplasmas and tissue culture cells. Nature. 1967 Dec 30;216(5122):1306–1307. doi: 10.1038/2161306a0. [DOI] [PubMed] [Google Scholar]
  30. Taylor-Robinson D., Manchee R. J. Spermadsorption and spermagglutination by mycoplasmas. Nature. 1967 Jul 29;215(5100):484–487. doi: 10.1038/215484a0. [DOI] [PubMed] [Google Scholar]
  31. Woodruff K. H., Schneider E., Unger L., Coalson J. J. Ultrastructural changes in hamster tracheal ring cultures exposed to Mycoplasma pneumoniae. Am J Pathol. 1973 Jul;72(1):91–102. [PMC free article] [PubMed] [Google Scholar]

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