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. 1979 Jun;24(3):932–938. doi: 10.1128/iai.24.3.932-938.1979

Phagocytosis and intracellular killing of pathogenic yeasts by human monocytes and neutrophils.

K E Schuit
PMCID: PMC414397  PMID: 381207

Abstract

The kinetics of phagocytosis and killing of four fungal forms with varying virulence by two types of phagocytic cells was examined. Human monocytes ingested Saccharomyces cerevisiae, Candida tropicalis, and the blastospores of Candida albicans more rapidly than did human neutrophils. There was no difference in the rate of phagocytosis of C. albicans pseudohyphae by these two cell types. Intracellular killing of each of the four fungal forms was consistently and significantly more rapid by monocytes than by neutrophils. Neutrophils were unable to destroy ingested C. albicans pseudohyphae. These experiments suggest that the monocyte plays an important role in host defenses against fungal diseases and that the relative virulence of the pathogenic yeasts in human disease may be related to the ability of these organisms to survival after being ingested by circulating phagocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belcher R. W., Carney J. F., Monahan F. G. An electron microscopic study of phagocytosis of Candida albicans by polymorphonuclear leukocytes. Lab Invest. 1973 Dec;29(6):620–627. [PubMed] [Google Scholar]
  2. Calderone R. A., Rotondo M. F., Sande M. A. Candida albicans endocarditis: ultrastructural studies of vegetation formation. Infect Immun. 1978 Apr;20(1):279–289. doi: 10.1128/iai.20.1.279-289.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cassone A., Simonetti N., Strippoli V. Ultrastructural changes in the wall during germ-tube formation from blastospores of Candida albicans. J Gen Microbiol. 1973 Aug;77(2):417–426. doi: 10.1099/00221287-77-2-417. [DOI] [PubMed] [Google Scholar]
  4. Cawson R. A., Rajasingham K. C. Ultrastructural features of the invasive phase of Candida albicans. Br J Dermatol. 1972 Nov;87(5):435–443. doi: 10.1111/j.1365-2133.1972.tb01591.x. [DOI] [PubMed] [Google Scholar]
  5. Cohen A. B., Cline M. J. The human alveolar macrophage: isolation, cultivation in vitro, and studies of morphologic and functional characteristics. J Clin Invest. 1971 Jul;50(7):1390–1398. doi: 10.1172/JCI106622. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edwards J. E., Jr, Lehrer R. I., Stiehm E. R., Fischer T. J., Young L. S. Severe candidal infections: clinical perspective, immune defense mechanisms, and current concepts of therapy. Ann Intern Med. 1978 Jul;89(1):91–106. doi: 10.7326/0003-4819-89-1-91. [DOI] [PubMed] [Google Scholar]
  7. GEBHARDT L. P., HILL D. W. Morphological transformation of Candida albicans in tissues of mice. Proc Soc Exp Biol Med. 1956 Jul;92(3):640–644. doi: 10.3181/00379727-92-22570. [DOI] [PubMed] [Google Scholar]
  8. Kim M. H., Rodey G. E., Good R. A., Chilgren R. A., Quie P. G. Defective candidacidal capacity of polymorphonuclear leukocytes in chronic granulomatous disease of childhood. J Pediatr. 1969 Aug;75(2):300–303. doi: 10.1016/s0022-3476(69)80403-8. [DOI] [PubMed] [Google Scholar]
  9. LOURIA D. B., BRAYTON R. G. BEHAVIOR OF CANDIDA CELLS WITHIN LEUKOCYTES. Proc Soc Exp Biol Med. 1964 Jan;115:93–98. doi: 10.3181/00379727-115-28840. [DOI] [PubMed] [Google Scholar]
  10. Lehrer R. I., Cline M. J. Interaction of Candida albicans with human leukocytes and serum. J Bacteriol. 1969 Jun;98(3):996–1004. doi: 10.1128/jb.98.3.996-1004.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lehrer R. I. Measurement of candidacidal activity of specific leukocyte types in mixed cell populations I. Normal, myeloperoxidase-deficient, and chronic granulomatous disease neutrophils. Infect Immun. 1970 Jul;2(1):42–47. doi: 10.1128/iai.2.1.42-47.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lehrer R. I. The fungicidal mechanisms of human monocytes. I. Evidence for myeloperoxidase-linked and myeloperoxidase-independent candidacidal mechanisms. J Clin Invest. 1975 Feb;55(2):338–346. doi: 10.1172/JCI107937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Leijh P. C., van den Barselaar M. T., van Furth R. Kinetics of phagocytosis and intracellular killing of Candida albicans by human granulocytes and monocytes. Infect Immun. 1977 Aug;17(2):313–318. doi: 10.1128/iai.17.2.313-318.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Orlowski J. P., Sieger L., Anthony B. F. Bactericidal capacity of monocytes of newborn infants. J Pediatr. 1976 Nov;89(5):797–801. doi: 10.1016/s0022-3476(76)80810-4. [DOI] [PubMed] [Google Scholar]
  15. Ozato K., Uesaka I. The role of macrophages in Candida albicans infection in vitro. Jpn J Microbiol. 1974 Jan;18(1):29–35. doi: 10.1111/j.1348-0421.1974.tb00740.x. [DOI] [PubMed] [Google Scholar]
  16. Peterson P. K., Verhoef J., Schmeling D., Quie P. G. Kinetics of phagocytosis and bacterial killing by human polymorphonuclear leukocytes and monocytes. J Infect Dis. 1977 Oct;136(4):502–509. doi: 10.1093/infdis/136.4.502. [DOI] [PubMed] [Google Scholar]
  17. Smith D. L., Rommel F. A rapid micro method for the simultaneous determination of phagocytic-microbiocidal activity of human peripheral blood leukocytes in vitro. J Immunol Methods. 1977;17(3-4):241–247. doi: 10.1016/0022-1759(77)90106-5. [DOI] [PubMed] [Google Scholar]
  18. Xanthou M., Valassi-Adam E., Kintsonidou E., Matsaniotis N. Phagocytosis and killing ability of Candida albicans by blood leucocytes of healthy term and preterm babies. Arch Dis Child. 1975 Jan;50(1):72–75. doi: 10.1136/adc.50.1.72. [DOI] [PMC free article] [PubMed] [Google Scholar]

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