Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1979 Aug;25(2):580–582. doi: 10.1128/iai.25.2.580-582.1979

Exposure of guinea pigs to Rickettsia rickettsii by aerosol, nasal, conjunctival, gastric, and subcutaneous routes and protection afforded by an experimental vaccine.

R H Kenyon, R A Kishimoto, W C Hall
PMCID: PMC414484  PMID: 114488

Abstract

Guinea pigs were inoculated with Rocky Mountain spotted fever by the aerosol, conjunctival, subcutaneous, intragastric, and intranasal routes. Rickettsial infection was produced by all routes except intragastric. All animals with clinical signs of disease developed agglutinating antibody, and most developed a cell-mediated immune response. Disease produced by all experimental routes (except intragastric) was indistinguishable. The tissue culture-derived inactivated vaccine produced in this laboratory protected guinea pigs against an aerosol challenge.

Full text

PDF
580

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ascher M. S., Oster C. N., Harber P. I., Kenyon R. H., Pedersen C. E., Jr Initial clinical evaluation of a new Rocky Mountain spotted fever vaccine of tissue culture origin. J Infect Dis. 1978 Aug;138(2):217–221. doi: 10.1093/infdis/138.2.217. [DOI] [PubMed] [Google Scholar]
  2. DuPont H. L., Hornick R. B., Dawkins A. T., Heiner G. G., Fabrikant I. B., Wisseman C. L., Jr, Woodward T. E. Rocky Mountain spotted fever: a comparative study of the active immunity induced by inactivated and viable pathogenic Rickettsia rickettsii. J Infect Dis. 1973 Sep;128(3):340–344. doi: 10.1093/infdis/128.3.340. [DOI] [PubMed] [Google Scholar]
  3. Fiset P., Ormsbee R. A., Silberman R., Peacock M., Spielman S. H. A microagglutination technique for detection and measurement of rickettsial antibodies. Acta Virol. 1969 Jan;13(1):60–66. [PubMed] [Google Scholar]
  4. Kenyon R. H., Acree W. M., Wright G. G., Melchior F. W., Jr Preparation of vaccines for Rocky Mountain spotted fever from rickettsiae propagated in cell culture. J Infect Dis. 1972 Feb;125(2):146–152. doi: 10.1093/infdis/125.2.146. [DOI] [PubMed] [Google Scholar]
  5. Kenyon R. H., Ascher M. S., Kishimoto R. A., Pedersen C. E., Jr In vitro guinea pig leukocyte reactions to Rickettsia rickettsii. Infect Immun. 1977 Dec;18(3):840–846. doi: 10.1128/iai.18.3.840-846.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kenyon R. H., Pedersen C. E., Jr Preparation of Rocky Mountain spotted fever vaccine suitable for human immunization. J Clin Microbiol. 1975 Jun;1(6):500–503. doi: 10.1128/jcm.1.6.500-503.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kenyon R. H., Sammons L. S., Pedersen C. E., Jr Comparison of three rocky mountain spotted fever vaccines. J Clin Microbiol. 1975 Oct;2(4):300–304. doi: 10.1128/jcm.2.4.300-304.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kishimoto R. A., Burger G. T. Appearance of cellular and humoral immunity in guinea pigs after infection with Coxiella burnetii administered in small-particle aerosols. Infect Immun. 1977 May;16(2):518–521. doi: 10.1128/iai.16.2.518-521.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Moe J. B., Mosher D. F., Kenyon R. H., White J. D., Stookey J. L., Bagley L. R., Fine D. P. Functional and morphologic changes during experimental Rocky Mountain spotted fever in guinea pigs. Lab Invest. 1976 Sep;35(3):235–245. [PubMed] [Google Scholar]
  10. Moe J. B., Ruch G. L., Kenyon R. H., Burek J. D., Stookey J. L. Pathology of experimental Rocky Mountain spotted fever in rhesus monkeys. Vet Pathol. 1976;13(1):69–77. doi: 10.1177/030098587601300108. [DOI] [PubMed] [Google Scholar]
  11. Oster C. N., Burke D. S., Kenyon R. H., Ascher M. S., Harber P., Pedersen C. E., Jr Laboratory-acquired Rocky Mountain spotted fever. The hazard of aerosol transmission. N Engl J Med. 1977 Oct 20;297(16):859–863. doi: 10.1056/NEJM197710202971604. [DOI] [PubMed] [Google Scholar]
  12. STOENNER H. G., LACKMAN D. B., BELL E. J. Factors affecting the growth of rickettsias of the spotted fever group in fertile hens' eggs. J Infect Dis. 1962 Mar-Apr;110:121–128. doi: 10.1093/infdis/110.2.121. [DOI] [PubMed] [Google Scholar]
  13. Sammons L. S., Kenyon R. H., Pedersen C. E., Jr Effect of vaccination schedule on immune response of Macaca mulatta to cell culture-grown Rocky Mountain spotted fever vaccine. J Clin Microbiol. 1976 Sep;4(3):253–257. doi: 10.1128/jcm.4.3.253-257.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Saslaw S., Carlisle H. N. Aerosol infection of monkeys with Rickettsia rickettsii. Bacteriol Rev. 1966 Sep;30(3):636–645. doi: 10.1128/br.30.3.636-645.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weinberg E. H., Stakebake J. R., Gerone P. J. Plaque assay for Rickettsia rickettsii. J Bacteriol. 1969 May;98(2):398–402. doi: 10.1128/jb.98.2.398-402.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wolf G. L., Cole C. R., Carlisle H. N., Saslaw S. The pathogenesis of Rocky Mountain spotted fever in monkeys, infected by inhalation. Arch Pathol. 1967 Nov;84(5):486–494. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES