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. 1979 Aug;25(2):685–693. doi: 10.1128/iai.25.2.685-693.1979

Capacity of anaerobic bacteria from necrotic dental pulps to induce purulent infections.

G K Sundqvist, M I Eckerbom, A P Larsson, U T Sjögren
PMCID: PMC414499  PMID: 489126

Abstract

Combinations of bacteria isolated from the root canals of teeth with necrotic pulps and periapical bone destruction were tested for their capacity to induce abscess formation and transmissible infections when inoculated subcutaneously into guinea pigs. Transmissible infections could be induced with combinations obtained from teeth with purulent apical inflammation, but not with combinations from symptomless teeth with chronic apical inflammation. All combinations which gave transmissible infections contained strains of Bacteroides melaninogenicus or B. asaccharolyticus (formerly B. melaninogenicus subsp. asaccharolyticus). The results suggest that purulent inflammation in the apical region in certain cases may be induced by specific combinations of bacteria in the root canal and that the presence of B. melaninogenicus or B. asaccharolyticus in such combinations is essential. However, with one exception, the strains needed the support of additional microorganisms to achieve pathogenicity. The results indicate that Peptostreptococcus micros was also essential. Histological sections of the lesions in the guinea pigs showed that all bacterial combinations induced acute inflammation with an accumulation of polymorphonuclear leukocytes and the formation of an abscess. However, the presence of B. melaninogenicus or B. asaccharolyticus in the combinations resulted in a failure of abscess resolution, with a gradually increaseing accumulation of polymorphonuclear leukocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergenholtz G. Micro-organisms from necrotic pulp of traumatized teeth. Odontol Revy. 1974;25(4):347–358. [PubMed] [Google Scholar]
  2. Christensen P., Kronvall G. Capacity of group A, B, C, D, and G streptococci to agglutinate sensitized sheep red cells. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Feb;82(1):19–24. doi: 10.1111/j.1699-0463.1974.tb02287.x. [DOI] [PubMed] [Google Scholar]
  3. Christensen P., Oxelius V. A. Quantitation of the uptake of human IgG by some streptococci groups A, B, C, and G. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Aug;82(4):475–483. doi: 10.1111/j.1699-0463.1974.tb02356.x. [DOI] [PubMed] [Google Scholar]
  4. Courant P. R., Gibbons R. J. Biochemical and immunological heterogeneity of Bacteroides melaninogenicus. Arch Oral Biol. 1967 Dec;12(12):1605–1613. doi: 10.1016/0003-9969(67)90194-x. [DOI] [PubMed] [Google Scholar]
  5. Forsgren A., Forsum U. Role of Protein A in Nonspecific Immunofluorescence of Staphylococcus aureus. Infect Immun. 1970 Oct;2(4):387–391. doi: 10.1128/iai.2.4.387-391.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Forsgren A., Sjöquist J. "Protein A" from S. aureus. I. Pseudo-immune reaction with human gamma-globulin. J Immunol. 1966 Dec;97(6):822–827. [PubMed] [Google Scholar]
  7. GIBBONS R. J., MACDONALD J. B. Degradation of collagenous substrates by Bacteroides melaninogenicus. J Bacteriol. 1961 Apr;81:614–621. doi: 10.1128/jb.81.4.614-621.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GIBBONS R. J., SOCRANSKY S. S., SAWYER S., KAPSIMALIS B., MACDONALD J. B. The microbiota of the gingival crevice area of man. II. The predominant cultivable organisms. Arch Oral Biol. 1963 May-Jun;8:281–289. doi: 10.1016/0003-9969(63)90020-7. [DOI] [PubMed] [Google Scholar]
  9. Hausmann E., Courant P. R., Arnold D. S. Conditions for the demonstration of collagenolytic activity in Bacteroides melaninogenicus. Arch Oral Biol. 1967 Feb;12(2):317–320. doi: 10.1016/0003-9969(67)90055-6. [DOI] [PubMed] [Google Scholar]
  10. Hausmann E., Kaufman E. Collagenase activity in a particulate fraction from Bacteroides melaninogenicus. Biochim Biophys Acta. 1969 Dec 23;194(2):612–615. doi: 10.1016/0005-2795(69)90127-5. [DOI] [PubMed] [Google Scholar]
  11. Hofstad T. Antibodies reacting with lipopolysaccharides from Bacteroides melaninogenicus, in serum from normal human subjects. J Infect Dis. 1974 Mar;129(3):349–352. doi: 10.1093/infdis/129.3.349. [DOI] [PubMed] [Google Scholar]
  12. Hofstad T. Serological properties of lipopolysaccharide from oral stains of Bacteroides melaninogenicus. J Bacteriol. 1969 Mar;97(3):1078–1082. doi: 10.1128/jb.97.3.1078-1082.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Holm S. E. Precipitinogens in beta-hemolytic streptococci and some related human kidney antigens. Acta Pathol Microbiol Scand. 1967;70(1):79–94. doi: 10.1111/j.1699-0463.1967.tb01272.x. [DOI] [PubMed] [Google Scholar]
  14. Kantz W. E., Henry C. A. Isolation and classification of anaerobic bacteria from intact pulp chambers of non-vital teeth in man. Arch Oral Biol. 1974 Jan;19(1):91–96. doi: 10.1016/0003-9969(74)90231-3. [DOI] [PubMed] [Google Scholar]
  15. Kronvall G. A surface component in group A, C, and G streptococci with non-immune reactivity for immunoglobulin G. J Immunol. 1973 Nov;111(5):1401–1406. [PubMed] [Google Scholar]
  16. Lambe D. W., Jr Determination of Bacteroides melaninogenicus serogroups by fluorescent antibody staining. Appl Microbiol. 1974 Oct;28(4):561–567. doi: 10.1128/am.28.4.561-567.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MACDONALD J. B., GIBBONS R. J. The relationship of indigenous bacteria to periodontal disease. J Dent Res. 1962 Jan-Feb;41:320–326. doi: 10.1177/00220345620410014201. [DOI] [PubMed] [Google Scholar]
  18. MACDONALD J. B., SUTTON R. M., KNOLL M. L. The production of fusospirochetal infections in guinea pigs with recombined pure cultures. J Infect Dis. 1954 Nov-Dec;95(3):275–284. doi: 10.1093/infdis/95.3.275. [DOI] [PubMed] [Google Scholar]
  19. MERGENHAGEN S. E., HAMPP E. G., SCHERP H. W. Preparation and biological activities of endotoxins from oral bacteria. J Infect Dis. 1961 May-Jun;108:304–310. doi: 10.1093/infdis/108.3.304. [DOI] [PubMed] [Google Scholar]
  20. Mansheim B. J., Kasper D. L. Purification and immunochemical characterization of the outer membrane complex of Bacteroides melaninogenicus subspecies asaccharolyticus. J Infect Dis. 1977 May;135(5):787–799. doi: 10.1093/infdis/135.5.787. [DOI] [PubMed] [Google Scholar]
  21. Okuda K., Takazoe I. Antiphagocytic effects of the capsular structure of a pathogenic strain of Bacteroides melaninogenicus. Bull Tokyo Dent Coll. 1973 Aug;14(3):99–104. [PubMed] [Google Scholar]
  22. Roberts D. S. Synergic mechanisms in certain mixed infections. J Infect Dis. 1969 Dec;120(6):720–724. doi: 10.1093/infdis/120.6.720. [DOI] [PubMed] [Google Scholar]
  23. Roberts D. S. The pathogenic synergy of Fusiformis necrophorus and Corynebacterium pyogenes. I. Influence of the leucocidal exotoxin of F. necrophorus. Br J Exp Pathol. 1967 Dec;48(6):665–673. [PMC free article] [PubMed] [Google Scholar]
  24. SOCRANSKY S. S., GIBBONS R. J., DALE A. C., BORTNICK L., ROSENTHAL E., MACDONALD J. B. The microbiota of the gingival crevice area of man. I. Total microscopic and viable counts and counts of specific organisms. Arch Oral Biol. 1963 May-Jun;8:275–280. doi: 10.1016/0003-9969(63)90019-0. [DOI] [PubMed] [Google Scholar]
  25. SOCRANSKY S. S., GIBBONS R. J. REQUIRED ROLE OF BACTEROIDES MELANINOGENICUS IN MIXED ANAEROBIC INFECTIONS. J Infect Dis. 1965 Jun;115:247–253. doi: 10.1093/infdis/115.3.247. [DOI] [PubMed] [Google Scholar]
  26. Slots J. The predominant cultivable microflora of advanced periodontitis. Scand J Dent Res. 1977 Jan-Feb;85(2):114–121. doi: 10.1111/j.1600-0722.1977.tb00541.x. [DOI] [PubMed] [Google Scholar]
  27. Takazoe I., Nakamura T. Experimental mixed infection by human gingival crevice material. Bull Tokyo Dent Coll. 1971 May;12(2):85–93. [PubMed] [Google Scholar]
  28. Takazoe I., Okuda K., Yammoto A. Distribution of a K-antigen among oral strains of Bacteroides melaninogenicus. Bull Tokyo Dent Coll. 1975 Feb;16(1):1–5. [PubMed] [Google Scholar]
  29. Vande Visse J. E., Brilliant J. D. Effect of irrigation on the production of extruded material at the root apex during instrumentation. J Endod. 1975 Jul;1(7):243–246. doi: 10.1016/s0099-2399(75)80227-5. [DOI] [PubMed] [Google Scholar]
  30. WADSWORTH C. A slide microtechnique for the analysis of immune precipitates in gel. Int Arch Allergy Appl Immunol. 1957;10(6):355–360. doi: 10.1159/000228394. [DOI] [PubMed] [Google Scholar]
  31. Williams B. L., Pantalone R. M., Sherris J. C. Subgingival microflora and periodontitis. J Periodontal Res. 1976 Feb;11(1):1–18. doi: 10.1111/j.1600-0765.1976.tb00045.x. [DOI] [PubMed] [Google Scholar]
  32. Wittgow W. C., Jr, Sabiston C. B., Jr Microorganisms from pulpal chambers of intact teeth with necrotic pulps. J Endod. 1975 May;1(5):168–171. doi: 10.1016/S0099-2399(75)80115-4. [DOI] [PubMed] [Google Scholar]

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