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. 1979 Sep;25(3):844–848. doi: 10.1128/iai.25.3.844-848.1979

Guinea Pig Lung Lavage Cells After Intranasal BCG Sensitization

T Terai 1, Rama Ganguly 2,3, Robert H Waldman 3
PMCID: PMC414525  PMID: 387595

Abstract

Recent studies have suggested that intranasal administration of antigen can induce local cell-mediated immunity in lung lavage cells. The present study was designed to examine the changes in composition of lung lavage cells and their capacity to produce the lymphokine migration inhibitory factor after intranasal immunization with BCG in guinea pigs. Results indicate that guinea pigs responded to respiratory tract BCG infection with an increase in immunocompetent cells in the bronchoalveolar tract and with production of migration inhibitory factor. After local pulmonary BCG administration, the total number of cells increased as compared with that of the uninfected animals, the increase being statistically significant within 2 weeks. This marked increase in the total cell population is due to a more than doubling of the number of macrophages in the lavage fluid. Animals also developed at this time positive delayed hypersensitivity to intradermally administered purified protein derivative. A significant increase in the total lymphoid cells and macrophage population was observed again at 6 weeks after sensitization, suggesting that the response is biphasic in nature. At 6 weeks, however, there was also a significant rise in total lymphocytes and T cell population in addition to macrophage numbers. This increase in T cells correlated with an increase in production of migration inhibitory factor in the presence of purified protein derivative. These data suggest that the immune response of the respiratory tract after BCG challenge involves increased recruitment of immunocompetent cells locally at the site of infection and that these cells are capable of producing effector molecules in terms of the elaboration of migration inhibitory factor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adamson I. Y., Bowden D. H. Adaptive responses of the pulmonary macrophagic system to carbon. II. Morphologic studies. Lab Invest. 1978 Apr;38(4):430–438. [PubMed] [Google Scholar]
  2. Bloom B. R., Bennett B. Mechanism of a reaction in vitro associated with delayed-type hypersensitivity. Science. 1966 Jul 1;153(3731):80–82. doi: 10.1126/science.153.3731.80. [DOI] [PubMed] [Google Scholar]
  3. Bowden D. H., Adamson I. Y. Adaptive responses of the pulmonary macrophagic system to carbon. I. Kinetic studies. Lab Invest. 1978 Apr;38(4):422–429. [PubMed] [Google Scholar]
  4. Daniele R. P., Altose M. D., Rowlands D. T., Jr Immunocompetent cells from the lower respiratory tract of normal human lungs. J Clin Invest. 1975 Oct;56(4):986–995. doi: 10.1172/JCI108179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Galindo B., Myrvik Q. N. Migratory response of granulomatous alveolar cells from BCG-sensitized rabbits. J Immunol. 1970 Jul;105(1):227–237. [PubMed] [Google Scholar]
  6. Ganguly R., Clem L. W., Bencić Z., Sinha R., Sakazaki R., Waldman R. H. Antibody response in the intestinal secretions of volunteers immunized with various cholera vaccines. Bull World Health Organ. 1975;52(3):323–330. [PMC free article] [PubMed] [Google Scholar]
  7. Ganguly R., Durrer B., Waldman R. H. Rubella virus immunization of preschool children via the respiratory tract. Am J Dis Child. 1974 Dec;128(6):821–823. doi: 10.1001/archpedi.1974.02110310069012. [DOI] [PubMed] [Google Scholar]
  8. Ganguly R., Ogra P. L., Regas S., Waldman R. H. Rubella immunization of volunteers via the respiratory tract. Infect Immun. 1973 Oct;8(4):497–502. doi: 10.1128/iai.8.4.497-502.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gershon R. K., Cohen P., Hencin R., Liebhaber S. A. Suppressor T cells. J Immunol. 1972 Mar;108(3):586–590. [PubMed] [Google Scholar]
  10. Gorenberg D. J., Daniele R. P. Characterization of immunocompetent cells recovered from the respiratory tract and tracheobronchial lymph node of normal guinea pigs. Am Rev Respir Dis. 1976 Dec;114(6):1099–1105. doi: 10.1164/arrd.1976.114.6.1099. [DOI] [PubMed] [Google Scholar]
  11. Kaltreider H. B., Salmon S. E. Immunology of the lower respiratory tract. Functional properties of bronchoalveolar lymphocytes obtained from the normal canine lung. J Clin Invest. 1973 Sep;52(9):2211–2217. doi: 10.1172/JCI107406. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kazmierowski J. A., Fauci A. S., Reynolds H. Y. Characterization of lymphocytes in bronchial lavage fluid from monkeys. J Immunol. 1976 Mar;116(3):615–618. [PubMed] [Google Scholar]
  13. Mackaness G. B. Resistance to intracellular infection. J Infect Dis. 1971 Apr;123(4):439–445. doi: 10.1093/infdis/123.4.439. [DOI] [PubMed] [Google Scholar]
  14. Moore V. L., Myrvik Q. N. The role of normal alveolar macrophages in cell-mediated immunity. J Reticuloendothel Soc. 1977 Feb;21(2):131–139. [PubMed] [Google Scholar]
  15. Perkins J. C., Tucker D. N., Knopf H. L., Wenzel R. P., Kapikian A. Z., Chanock R. M. Comparison of protective effect of neutralizing antibody in serum and nasal secretions in experimental rhinovirus type 13 illness. Am J Epidemiol. 1969 Dec;90(6):519–526. doi: 10.1093/oxfordjournals.aje.a121098. [DOI] [PubMed] [Google Scholar]
  16. Ribi E., Anacker R. L., Brehmer W., Goode G., Larson C. L., List R. H., Milner K. C., Wicht W. C. Factors influencing protection against experimental tuberculosis in mice by heat-stable cell wall vaccines. J Bacteriol. 1966 Oct;92(4):869–879. doi: 10.1128/jb.92.4.869-879.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Spencer J. C., Waldman R. H., Johnson J. E., 3rd Local and systemic cell-mediated immunity after immunization of guinea pigs with live or killed m. tuberculosis by various routes. J Immunol. 1974 Apr;112(4):1322–1328. [PubMed] [Google Scholar]
  18. Waldman R. H., Ganguly R. Immunity to infections on secretory surfaces. J Infect Dis. 1974 Oct;130(4):419–440. doi: 10.1093/infdis/130.4.419. [DOI] [PubMed] [Google Scholar]
  19. Yamamoto K., Anacker R. L., Ribi E. Macrophage Migration Inhibition Studies with Cells from Mice Vaccinated with Cell Walls of Mycobacterium bovis BCG: Relationship Between Inhibitory Activity of Lung Cells and Resistance to Airborne Challenge with Mycobacterium tuberculosis H37Rv. Infect Immun. 1970 Jun;1(6):595–599. doi: 10.1128/iai.1.6.595-599.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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