Abstract
Heat-killed whole BCG cells (KC) and BCG cell walls (CW) were each tested in emulsified form for their potency to cause regression of a transplanted guinea pig hepatoma. On a weight basis, KC were at least as effective as CW in causing tumor regression and elimination of microscopic lymph node metastasis, and they, as well as purified protein derivative of mycobacteria, provoked delayed cutaneous hypersensitivity reactions in animals immunized with CW or with KC. On a weight basis, KC were as active as CW in eliciting delayed cutaneous hypersensitivity in sensitized guinea pigs whether the animals were immunized with CW or with KC. In unimmunized animals the inflammatory response to intradermally administered KC was similar to that induced by CW. Because KC are easier to prepare than CW, it is suggested that whole killed BCG might be used instead of CW in clinical trials of cancer treatment requiring administration of nonliving mycobacteria.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Azuma I., Ribi E. E., Meyer T. J., Zbar B. Biologically active components from mycobacterial cell walls. I. Isolation and composition of cell wall skeleton and component P3. J Natl Cancer Inst. 1974 Jan;52(1):95–101. doi: 10.1093/jnci/52.1.95. [DOI] [PubMed] [Google Scholar]
- Baldwin R. W., Cook A. J., Hopper D. G., Pimm M. V. Radiation-killed BCG in the treatment of transplanted rat tumours. Int J Cancer. 1974 Jun 15;13(6):743–750. doi: 10.1002/ijc.2910130602. [DOI] [PubMed] [Google Scholar]
- Bekierkunst A., Wang L., Toubiana R., Lederer E. Immunotherapy of Cancer with Nonliving BCG and Fractions Derived from Mycobacteria: Role of Cord Factor (Trehalose-6, 6'-Dimycolate) in Tumor Regression. Infect Immun. 1974 Nov;10(5):1044–1050. doi: 10.1128/iai.10.5.1044-1050.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kleinschuster S. J., Rapp H. J., Lueker D. C., Kainer R. A. Regression of bovine ocular carcinoma by treatment with a mycobacterial vaccine. J Natl Cancer Inst. 1977 Jun;58(6):1807–1814. doi: 10.1093/jnci/58.6.1807. [DOI] [PubMed] [Google Scholar]
- Rapp H. J. A guinea pig model for tumor immunology. A summary. Isr J Med Sci. 1973 Mar;9(3):366–374. [PubMed] [Google Scholar]
- Yarkoni E., Rapp H. J. Influence of oil concentration on the efficacy of tumor regression by emulsified components of mycobacteria. Cancer Res. 1979 Feb;39(2 Pt 1):535–537. [PubMed] [Google Scholar]
- Zbar B., Ribi E., Meyer T., Azuma I., Rapp H. J. Immunotherapy of cancer: regression of established intradermal tumors after intralesional injection of mycobacterial cell walls attached to oil droplets. J Natl Cancer Inst. 1974 May;52(5):1571–1577. doi: 10.1093/jnci/52.5.1571. [DOI] [PubMed] [Google Scholar]