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. 1979 Oct;26(1):42–48. doi: 10.1128/iai.26.1.42-48.1979

Radioimmunoassay for Gram-negative bacterial lipopolysaccharide O antigens: influence of antigen solubility.

R S Munford, C L Hall
PMCID: PMC414572  PMID: 387612

Abstract

We have developed a solid-phase radioimmunoassay technique for specific gram-negative bacterial lipopolysaccharide (LPS) O antigens. The method exploits the high-titer, specific immunoglobulin M response of the rabbit to LPS immunization to measure as little as 5 ng of homologous LPS per ml with less than 0.5% cross-reactivity toward heterologous LPS or culture supernatants. We found that O antigen in complete LPS was less available for antibody binding than O antigen in the soluble polysaccharide derived by mild acid hydrolysis of LPS and that triethylamine-induced disaggregation of complete LPS increased its activity in the assay. Quantitation of O antigen with the assay was thus influenced by the physical state of LPS or "free" O antigen.

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Selected References

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