Abstract
Attachment of M. pneumoniae to glass was quantitated in an experimental system enabling the settling down of [3H]palmitic acid-labeled cells onto glass cover slips. Attachment of mycoplasmas suspended in buffer increased with temperature, decreased with higher ionic strength, and showed a maximum at about pH 5.5. The findings suggest a participation of ionic bonds in the attachment process. Trypsin did not detach glass-bound mycoplasmas, and treatment of the cells with glutaraldehyde did not reduce their attachment to glass, suggesting that membrane components other than proteins may be involved in the attachment. Low concentrations (up to 20 mg/ml) of bovine serum albumin buffer. However, during the next few hours, attachment increased far above the bovine serum albumin control. This marked increase was reduced by more than half in the presence of chloramphenicol. Increased attachment was also observed when glucose (0.1 to 2 mg/ml) was added to the bovine serum albumin-containing buffer. The findings suggest different mechanisms for the attachment in protein-free buffer and in growth medium or glucose-containing bovine serum albumin buffer, respectively. The latter apparently requires metabolic activity of the mycoplasmas.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Banai M., Kahane I., Razin S., Bredt W. Adherence of Mycoplasma gallisepticum to human erythrocytes. Infect Immun. 1978 Aug;21(2):365–372. doi: 10.1128/iai.21.2.365-372.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bredt W. Growth morphology of Mycoplasma pneumoniae strain FH on glass surface. Proc Soc Exp Biol Med. 1968 Jun;128(2):338–340. doi: 10.3181/00379727-128-33009. [DOI] [PubMed] [Google Scholar]
- Feldner J., Bredt W., Kahane I. Adherence of erythrocytes to Mycoplasma pneumoniae. Infect Immun. 1979 Jul;25(1):60–67. doi: 10.1128/iai.25.1.60-67.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hayflick L. Tissue cultures and mycoplasmas. Tex Rep Biol Med. 1965 Jun;23(Suppl):285+–285+. [PubMed] [Google Scholar]
- Kahane I., Gat O., Banai M., Bredt W., Razin S. Adherence of Mycoplasma gallisepticum to glass. J Gen Microbiol. 1979 Mar;111(1):217–222. doi: 10.1099/00221287-111-1-217. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Radestock U., Bredt W. Motility of Mycoplasma pneumoniae. J Bacteriol. 1977 Mar;129(3):1495–1501. doi: 10.1128/jb.129.3.1495-1501.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sobeslavsky O., Prescott B., Chanock R. M. Adsorption of Mycoplasma pneumoniae to neuraminic acid receptors of various cells and possible role in virulence. J Bacteriol. 1968 Sep;96(3):695–705. doi: 10.1128/jb.96.3.695-705.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Somerson N. L., James W. D., Walls B. E., Chanock R. M. Growth of Mycoplasma pneumoniae on a glass surface. Ann N Y Acad Sci. 1967 Jul 28;143(1):384–389. doi: 10.1111/j.1749-6632.1967.tb27680.x. [DOI] [PubMed] [Google Scholar]
- Taylor-Robinson D., Manchee R. J. Adherence of mycoplasmas to glass and plastic. J Bacteriol. 1967 Nov;94(5):1781–1782. doi: 10.1128/jb.94.5.1781-1782.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]