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. 1979 Oct;26(1):217–224. doi: 10.1128/iai.26.1.217-224.1979

Cell-mediated immunity to cytomegalovirus in immunocompromised patients: effect of radiation or chemotherapy.

Y Agatsuma, P Fitzpatrick, A Kaul, R Kaul, R Kohli, A V Paves, P L Ogra
PMCID: PMC414598  PMID: 227793

Abstract

Using the techniques of complement fixation, immunofluorescence, and in vitro lymphocyte transformation (LTF), we studied the humoral antibody and cell-mediated immunity to cytomegalovirus (CMV) in normal subjects, in patients with cancer receiving localized or nonlocalized radiation, and in renal transplant recipients on immunosuppressive chemotherapy. The LTF activity was determined by the whole blood microassay, using four strains of CMV (AD-169, Davis, Veca, and Towne), AD-169 early antigen, and phytohemagglutinin (PAH). The renal transplant subjects manifested significantly depressed LTF responses to PHA and CMV and frequent presence of immunoglobulin M and early antigen-specific antibody response. The depressed LTF response to CMV recovered significantly 2 years after transplantation. The cancer patients were also characterized by a profound drop in LTF responses to PHA and CMV and in immunoglobulin M and early antigen-specific antibody response after nonlocalized radiation. LTF responses to AD-169 and Towne strains were found to be higher than those to Davis and Veca strains. The LTF response to PHA declined with age. However, LTF responses to specific CMV antigens were found to be somewhat increased with advancing age. These observations suggest that transplantation-associated suppression of CMV-specific cell-mediated immunity may improve a few years after transplantation despite continued immunosuppressive therapy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdallah P. S., Mark J. B., Merigan T. C. Diagnosis of cytomegalovirus pneumonia in compromised hosts. Am J Med. 1976 Sep;61(3):326–332. doi: 10.1016/0002-9343(76)90368-5. [DOI] [PubMed] [Google Scholar]
  2. Andersen H. K., Spencer E. S. Cytomegalovirus infection among renal allograft recipients. Acta Med Scand. 1969 Jul-Aug;186(1-2):7–19. doi: 10.1111/j.0954-6820.1969.tb01432.x. [DOI] [PubMed] [Google Scholar]
  3. Armstrong D., Balakrishnan S. L., Steger L., Yu B., Stenzel K. H. Cytomegalovirus infections with viremia following renal transplantation. Arch Intern Med. 1971 Jan;127(1):111–115. [PubMed] [Google Scholar]
  4. BODEY G. P., WERTLAKE P. T., DOUGLAS G., LEVIN R. H. CYTOMEGALIC INCLUSION DISEASE IN PATIENTS WITH ACUTE LEUKEMIA. Ann Intern Med. 1965 May;62:899–906. doi: 10.7326/0003-4819-62-5-899. [DOI] [PubMed] [Google Scholar]
  5. Betts R. F., Freeman R. B., Douglas R. G., Jr, Talley T. E. Clinical manifestations of renal allograft derived primary cytomegalovirus infection. Am J Dis Child. 1977 Jul;131(7):759–763. doi: 10.1001/archpedi.1977.02120200041010. [DOI] [PubMed] [Google Scholar]
  6. Beutner K. R., Morag A., Deibel R., Morag B., Raiken D., Ogra P. L. Strain-specific local and systemic cell-mediated immune responses to cytomegalovirus in humans. Infect Immun. 1978 Apr;20(1):82–87. doi: 10.1128/iai.20.1.82-87.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cangir A., Sullivan M. P. The occurrence of cytomegalovirus infections in childhood leukemia. JAMA. 1966 Feb 21;195(8):616–622. [PubMed] [Google Scholar]
  8. Caul E. O., Dickinson V. A., Roome A. P., Mott M. G., Stevenson P. A. Cytomegalovirus infections in leukaemic children. Int J Cancer. 1972 Jul 15;10(1):213–220. doi: 10.1002/ijc.2910100127. [DOI] [PubMed] [Google Scholar]
  9. Chess L., MacDermott R. P., Schlossman S. F. Immunologic functions of isolated human lymphocyte subpopulations. II. Antigen triggering of T and B cells in vitro. J Immunol. 1974 Oct;113(4):1122–1127. [PubMed] [Google Scholar]
  10. Chiba Y., Fitzpatrick P., Patel P., Scott R., Ogra P. L. Age related differences in cellular immune response to vaccine induced rubella infection. Microbiol Immunol. 1978;22(6):325–334. doi: 10.1111/j.1348-0421.1978.tb00377.x. [DOI] [PubMed] [Google Scholar]
  11. Cremer N. E., Schmidt N. J., Jensen F., Hoffman M., Oshiro L. S., Lennette E. H. Complement-fixing antibody in human sera reactive with viral and soluble antigens of cytomegalovirus. J Clin Microbiol. 1975 Mar;1(3):262–267. doi: 10.1128/jcm.1.3.262-267.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Dowling J. N., Saslow A. R., Armstrong J. A., Ho M. Cytomegalovirus infection in patients receiving immunosuppressive therapy for rheumatologic disorders. J Infect Dis. 1976 Apr;133(4):399–408. doi: 10.1093/infdis/133.4.399. [DOI] [PubMed] [Google Scholar]
  13. Fiala M., Payne J. E., Berne T. V., Moore T. C., Henle W., Montgomerie J. Z., Chatterjee S. N., Guze L. B. Epidemiology of cytomegalovirus infection after transplantation and immunosuppression. J Infect Dis. 1975 Oct;132(4):421–433. doi: 10.1093/infdis/132.4.421. [DOI] [PubMed] [Google Scholar]
  14. Fine R. N., Grushkin C. M., Malekzadeh M., Wright H. T., Jr Cytomegalovirus syndrome following renal transplantation. Arch Surg. 1972 Oct;105(4):564–570. doi: 10.1001/archsurg.1972.04180100015006. [DOI] [PubMed] [Google Scholar]
  15. Foad B. S., Adams L. E., Yamauchi Y., Litwin A. Phytomitogen responses of peripheral blood lymphocytes in young and older subjects. Clin Exp Immunol. 1974 Aug;17(4):657–664. [PMC free article] [PubMed] [Google Scholar]
  16. Folb P. I., Trounce J. R. Immunological aspects of candida infection complicating steroid and immunosuppressive drug therapy. Lancet. 1970 Nov 28;2(7683):1112–1114. doi: 10.1016/s0140-6736(70)92299-3. [DOI] [PubMed] [Google Scholar]
  17. GOTTMANN A. W., BEATTY E. C., Jr Cytomegalic inclusion disease in children with leukemia or lymphosarcoma. Am J Dis Child. 1962 Aug;104:180–184. doi: 10.1001/archpedi.1962.02080030182012. [DOI] [PubMed] [Google Scholar]
  18. Identification, enumeration, and isolation of B and T lymphocytes from human peripheral blood. Report of a WHO-IARC-sponsored workshop on human B and T cells, London, 15-17 July 1974. Scand J Immunol. 1974;3(5):521–532. [PubMed] [Google Scholar]
  19. Krech U., Jung M., Sonnabend W. A study of complement-fixing, immunofluorescent, and neutralizing antibodies in human cytomegalovirus infections. Z Immunitatsforsch Exp Klin Immunol. 1971 Jun;141(5):411–429. [PubMed] [Google Scholar]
  20. Park B. H., Good R. A. A new micromethod for evaluating lymphocyte responses to phytohemagglutinin: quantitative analysis of the function of thymus-dependent cells. Proc Natl Acad Sci U S A. 1972 Feb;69(2):371–373. doi: 10.1073/pnas.69.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pauly J. L., Han T. Whole blood microculture assay of human lymphocyte function. J Lab Clin Med. 1976 Nov;88(5):864–872. [PubMed] [Google Scholar]
  22. Pien F. D., Smith T. F., Anderson C. F., Webel M. L., Taswell H. F. Herpesviruses in renal transplant patients. Transplantation. 1973 Nov;16(5):489–495. doi: 10.1097/00007890-197311000-00014. [DOI] [PubMed] [Google Scholar]
  23. Pollard R. B., Rand K. H., Arvin A. M., Merigan T. C. Cell-mediated immunity of cytomegalovirus infection in normal subjects and cardiac transplant patients. J Infect Dis. 1978 May;137(5):541–549. doi: 10.1093/infdis/137.5.541. [DOI] [PubMed] [Google Scholar]
  24. Roberts-Thomson I. C., Whittingham S., Youngchaiyud U., Mackay I. R. Ageing, immune response, and mortality. Lancet. 1974 Aug 17;2(7877):368–370. doi: 10.1016/s0140-6736(74)91755-3. [DOI] [PubMed] [Google Scholar]
  25. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  26. Suwansirikul S., Rao N., Dowling J. N., Ho M. Primary and secondary cytomegalovirus infection. Arch Intern Med. 1977 Aug;137(8):1026–1029. [PubMed] [Google Scholar]
  27. The T. H., Andersen H. K., Spencer E. S., Klein G. Antibodies against cytomegalovirus-induced early antigens (CMV-EA) in immunosuppressed renal-allograft recipients. Clin Exp Immunol. 1977 Jun;28(3):502–505. [PMC free article] [PubMed] [Google Scholar]
  28. The T. H., Klein G., Langenhuysen M. M. Antibody reactions to virus-specific early antigens (EA) in patients with cytomegalovirus (CMV) infection. Clin Exp Immunol. 1974 Jan;16(1):1-7,9-12. [PMC free article] [PubMed] [Google Scholar]
  29. WELLER T. H., HANSHAW J. B., SCOTT D. E. Serologic differentiation of viruses responsible for cytomegalic inclusion disease. Virology. 1960 Sep;12:130–132. doi: 10.1016/0042-6822(60)90156-2. [DOI] [PubMed] [Google Scholar]
  30. Waner J. L., Weller T. H. Analysis of antigenic diversity among human cytomegaloviruses by kinetic neutralization tests with high-titered rabbit antisera. Infect Immun. 1978 Jul;21(1):151–157. doi: 10.1128/iai.21.1.151-157.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Weksler M. E., Hütteroth T. H. Impaired lymphocyte function in aged humans. J Clin Invest. 1974 Jan;53(1):99–104. doi: 10.1172/JCI107565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. West W. H., Sienknecht C. W., Townes A. S., Herberman R. B. Performance of a rosette assay between lymphocytes and sheep erythrocytes at elevated temperatures to study patients with cancer and other diseases. Clin Immunol Immunopathol. 1976 Jan;5(1):60–66. doi: 10.1016/0090-1229(76)90149-5. [DOI] [PubMed] [Google Scholar]
  33. Zablotney S. L., Wentworth B. B., Alexander E. R. Antigenic relatedness of 17 strains of human cytomegalovirus. Am J Epidemiol. 1978 Apr;107(4):336–343. doi: 10.1093/oxfordjournals.aje.a112549. [DOI] [PubMed] [Google Scholar]

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